Results 61 to 70 of about 252,228 (285)

LDAcoop: Integrating non‐linear population dynamics into the analysis of clonogenic growth in vitro

Molecular Oncology, EarlyView.
Limiting dilution assays (LDAs) quantify clonogenic growth by seeding serial dilutions of cells and scoring wells for colony formation. The fraction of negative wells is plotted against cells seeded and analyzed using the non‐linear modeling of LDAcoop.
Nikko Brix, Daniel Samaga, Katharina Gehr, Benedek Dankó, Mohamed Schumann, Guido Drexler, Ahmed Alnatsha, Georg Beyer, Ujjwal Mahajan, Martin Selmansberger, Julia Mayerle, Claus Belka, Horst Zitzelsberger, Kirsten Lauber   +13 more
wiley   +1 more source

Genomic deletions in Aureobasidium pullulans by an AMA1 plasmid for gRNA and CRISPR/Cas9 expression

Fungal Biology and Biotechnology
Background Aureobasidium pullulans is a generalist polyextremotolerant black yeast fungus. It tolerates temperatures below 0 °C or salt concentrations up to 18%, among other stresses. A. pullulans genome sequencing revealed a high potential for producing
Audrey Masi, Klara Wögerbauer, Robert L. Mach, Astrid R. Mach-Aigner   +3 more
doaj   +1 more source

Global Profiling of Carbohydrate Active Enzymes in Human Gut Microbiome.

PLoS ONE, 2015
MotivationCarbohydrate Active enzyme (CAZyme) families, encoded by human gut microflora, play a crucial role in breakdown of complex dietary carbohydrates into components that can be absorbed by our intestinal epithelium.
Tanudeep Bhattacharya, Tarini Shankar Ghosh, Sharmila S Mande   +2 more
doaj   +1 more source

Sterol 3β-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone [PDF]

, 2013
The main objectives of this work were to characterise a range of purified recombinant sterol 3β-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3β-glucosyltransferase sequence space can result in a range of
Altschul, Anthony Flinn, Bellu, Bonsor, Cantarel, Coutinho, Doerks, Gary W. Black, Grille, Hansen, Hashimoto, Julian S. Northen, Justin J. Perry, Laemmli, Lemmon, Li, Lynn G. Dover, Madina, Madina, Meng Zhang, O'Reilly, Oku, Palcic, Poppenberger, Rabbani, Robertson, Vatsala Malik, Warnecke, Weijers, Yang, Zea   +30 more
core   +1 more source

Dammarenediol II enhances etoposide‐induced apoptosis by targeting O‐GlcNAc transferase and Akt/GSK3β/mTOR signaling in liver cancer

Molecular Oncology, EarlyView.
Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee, Byung‐Cheol Han, Gi‐Bang Koo, Jihye Park, Mijin Kwon, Young Bin Park, Jae‐Mun Choi, Seung‐Ho Lee, Sangho Roh   +8 more
wiley   +1 more source

Characterization of strain degeneration in the RL-P37 strain lineage of Trichoderma reesei

Biotechnology for Biofuels and Bioproducts
Spontaneous strain degeneration, defined as the loss of an essential biological function during prolonged usage, is frequently observed in microorganisms and poses a significant challenge to the biotechnology industry.
Caroline Danner, Armin Gabriel, Christian Zimmermann, Robert L. Mach, Yuriy Karpenko, Igor Nikolaev, Sharief Barends, Astrid R. Mach-Aigner   +7 more
doaj   +1 more source

Systems approaches to predict the functions of glycoside hydrolases during the life cycle of Aspergillus niger using developmental mutants ∆brlA and ∆flbA.

PLoS ONE, 2015
BackgroundThe filamentous fungus Aspergillus niger encounters carbon starvation in nature as well as during industrial fermentations. In response, regulatory networks initiate and control autolysis and sporulation.
Jolanda M van Munster, Benjamin M Nitsche, Michiel Akeroyd, Lubbert Dijkhuizen, Marc J E C van der Maarel, Arthur F J Ram   +5 more
doaj   +1 more source

Metatranscriptomic holobiont analysis of carbohydrate-active enzymes in the millipede Telodeinopus aoutii (Diplopoda, Spirostreptida)

Frontiers in Ecology and Evolution, 2022
As important decomposers of soil organic matter, millipedes contribute to lignocellulose decomposition and nutrient cycling. The degradation of lignocellulose requires the action of several carbohydrate-active enzymes (CAZymes) and, in most invertebrates,
Puspendu Sardar, Vladimír Šustr, Alica Chroňáková, František Lorenc, František Lorenc   +4 more
doaj   +1 more source

Carbohydrate-active Enzymes from Extremophiles

Journal of Applied Glycoscience, 2006
The term “extremophiles” is used for organisms that thrive under extreme conditions, and are designated the enzymes that are active and tolerant under extreme reaction conditions “extremozymes”. We have long been engaged in the screening, gene cloning, and industrial applications of alkaline enzymes from alkaliphilic bacilli.
openaire   +2 more sources

Poplar Carbohydrate-Active Enzymes. Gene Identification and Expression Analyses [PDF]

Plant Physiology, 2006
Abstract Over 1,600 genes encoding carbohydrate-active enzymes (CAZymes) in the Populus trichocarpa (Torr. & Gray) genome were identified based on sequence homology, annotated, and grouped into families of glycosyltransferases, glycoside hydrolases, carbohydrate esterases, polysaccharide lyases, and expansins.
Jane, Geisler-Lee, Matt, Geisler, Pedro M, Coutinho, Bo, Segerman, Nobuyuki, Nishikubo, Junko, Takahashi, Henrik, Aspeborg, Soraya, Djerbi, Emma, Master, Sara, Andersson-Gunnerås, Björn, Sundberg, Stanislaw, Karpinski, Tuula T, Teeri, Leszek A, Kleczkowski, Bernard, Henrissat, Ewa J, Mellerowicz   +15 more
openaire   +2 more sources