Results 91 to 100 of about 47,659 (268)

Quantitative Competition Binding of Fluorinated Ligands by Real‐Time 19F In‐Cell NMR to Assess Isoform Selectivity in Human Cells

Magnetic Resonance in Chemistry, EarlyView.
Real‐time in‐cell 19F NMR spectroscopy in a flow NMR bioreactor was used to monitor the binding of fluorinated compounds to three cytosolic carbonic anhydrase isoforms in living human cells. The simultaneous observation of the two ligands allowed for the quantitative ranking of their intracellular affinities by competition binding. This approach allows
Azzurra Costantino, Letizia Barbieri, Simone Giovannuzzi, Alessio Nocentini, Claudiu T. Supuran, Enrico Luchinat   +5 more
wiley   +1 more source

Cigarette smoke extract induces a phenotypic shift in epithelial cells: involvement of HIF1α in mesenchymal transition [PDF]

, 2014
In COPD, matrix remodeling contributes to airflow limitation. Recent evidence suggests that next to fibroblasts, the process of epithelial-mesenchymal transition can contribute to matrix remodeling.
Bracke, Ken, de Theije, CC, Dentener, Mieke A, Eurlings, Irene MJ, Gosker, Harry R, Reynaert, Niki L, van den Beucken, Twan, Verhamme, Fien, Wouters, Emiel FM   +8 more
core   +3 more sources

Migrasomes, Matrix‐Bound Nanovesicles, and More: Messengers in the Matrix

PROTEOMICS, EarlyView.
ABSTRACT Extracellular vesicles (EVs) and particles (EPs) are diverse micro‐ and nanoparticles that circulate in bodily fluids and can attach to, or be deposited onto, the extracellular matrix (ECM) and other surfaces. To date, the nomenclature and classification of matrix‐bound or matrix‐associated EVs and EPs (MEVPs) have been unclear, largely due to
Anna V. Kolesov, Natalie Reitz, Marley Dewey, Colin L. Hisey   +3 more
wiley   +1 more source

Biophysical evidence for intrinsic disorder in the C-terminal tails of the epidermal growth factor receptor (EGFR) and HER3 receptor tyrosine kinases [PDF]

, 2016
The epidermal growth factor receptor (EGFR)/ErbB family of receptor tyrosine kinases includes oncogenes important in the progression of breast and other cancers, and they are targets for many drug development strategies.
Bose, Ron, Keppel, Theodore R, Monsey, John, Murray, Elisa M, Sarpong, Kwabena, Zhu, Jian   +5 more
core   +2 more sources

Parallel Analysis of Acidic and Basic Proteoforms in Cell Lysates via Native Cation and Anion Exchange Chromatography—Native Mass Spectrometry

PROTEOMICS, EarlyView.
ABSTRACT Comprehensive characterization of proteoforms in complex biological systems remains a significant challenge. While native ion‐exchange chromatography (IEC) hyphenated with native mass spectrometry (nMS) is a powerful tool for resolving proteoforms, three critical limitations persist: restricted volatile buffers causing nonlinear pH gradients ...
Ziran Zhai, Hafsa Zakri, Matteo Damian, Francesco G. Mutti, Garry Corthals, Andrea F. G. Gargano   +5 more
wiley   +1 more source

Phthalate Metabolite, Mono(2‐Ethyl‐5‐Hydroxyhexyl) Phthalate (MEHHP), Promotes Uterine‐Fibroid–Associated Phenotypes in Myometrial Stem Cell‐Derived 3D Organoids

Environmental Toxicology, EarlyView.
ABSTRACT This study investigates how phthalate exposure contributes to uterine fibroid (UF) development by studying the effects of the Mono‐(2‐ethyl‐5‐hydroxyhexyl) phthalate (MEHHP), a metabolite of Di(2‐ethylhexyl) phthalate, on myometrial stem cells (MMSCs).
Somayeh Vafaei, Mervat M. Omran, Qiwei Yang, Sandra Madueke‐Laveaux, Ami R. Zota, Ayman Al‐Hendy, Mohamed Ali   +6 more
wiley   +1 more source

Improving the 'tool box' for robust industrial enzymes. [PDF]

, 2017
This is the final version of the article. Available from Springer Verlag via the DOI in this record.The speed of sequencing of microbial genomes and metagenomes is providing an ever increasing resource for the identification of new robust biocatalysts ...
Littlechild, JA
core   +1 more source

Structures of variants of Escherichia coli flavodiiron‐type nitric oxide reductase reveal changes in the di‐iron site

Acta Crystallographica Section D, EarlyView.
Flavodiiron proteins (FDPs) are di‐iron enzymes that reduce NO and/or O2 with distinct substrate selectivity. We have produced E. coli FDP variants targeting second coordination‐sphere residues and determined their structures. Although the kinetics remained unchanged, the E.
Patrícia T. Borges, Filipe Folgosa, Maria C. Martins, Guillaume Gotthard, Peter van der Linden, Philippe Carpentier, Miguel Teixeira, Carlos Frazão, Célia V. Romão   +8 more
wiley   +1 more source

Major contribution of the type II beta carbonic anhydrase CanB (Cj0237) to the capnophilic growth phenotype of Campylobacter jejuni [PDF]

, 2016
Campylobacter jejuni, the leading cause of human bacterial gastroenteritis, requires low environmental oxygen and high carbon dioxide for optimum growth, but the molecular basis for the carbon dioxide requirement is unclear. One factor may be inefficient
Alazzam, Alber, Allos, Bolton, Bury-Moné, Capasso, Carta, Covarrubias, Cronk, Cronk, De Simone, Dugar, Eichler, Epps, Fernandez, Ferry, Fraser, Gai, Gaskin, Gibson, Gielda, Guccione, Guilloton, Gundogdu, Hani, Hermans, Hofreuter, Hofreuter, Hofreuter, Innocenti, Jitrapakdee, Kakuda, Kaur, Kelley, Kelly, Kelly, Kendall, Kusian, Lotlikar, Marcus, Menefee, Merlin, Muraoka, Ozensoy Guler, Parkhill, Pinard, Sheppard, Smith, Sokol, St Maurice, Stahl, Stahl, Stähler, Supuran, Supuran, Supuran, Velayudhan, Velayudhan, Vilas, Vliet, Vorwerk, Wilks   +61 more
core   +1 more source

Microarrayed Allergen Molecules Distinguish IgE Sensitisation to Blomia tropicalis and Dermatophagoides pteronyssinus

Allergy, EarlyView.
This study established a microarray containing a representative panel of purified Blo t and Der p allergens for the identification of patients sensitised to Blo t and/or Der p. Results indicate that the microarray based on Blo t and Der p allergens is a useful tool for the identification of Blo t‐ and/or Der p‐sensitised patients. The microarray may be
Nishelle Dsouza, Siratcha Phanthong, Huey‐Jy Huang, Milena Weber, Eszter Sarzsinszky, Petra Zieglmayer, Gabrielle Pauli, Margarete Focke‐Tejkl, Mikhail Tulaev, Nikolina Todorovic, Walter Keller, Thomas Schlederer, Luis Caraballo, Anchalee Tungtrongchitr, Rudolf Valenta, Pongsakorn Tantilipikorn, Susanne Vrtala   +16 more
wiley   +1 more source