Results 31 to 40 of about 312,617 (308)

Manipulation of Carrier Proteins in Antibiotic Biosynthesis [PDF]

Chemistry & Biology, 2004
Engineering biosynthetic pathways into suitable host organisms has become an attractive venue for the design, evaluation, and production of small molecule therapeutics. Polyketide (PK) and nonribosomal peptide (NRP) synthases have been of particular interest due to their modular structure, yet routine cloning and expression of these enzymes remains ...
Timothy L. Foley, James J. La Clair, Michael D. Burkart, Conor M Regan, Tracy R Schegg   +4 more
openaire   +3 more sources

HetI-Like Phosphopantetheinyl Transferase Posttranslationally Modifies Acyl Carrier Proteins in Xanthomonas spp.

Molecular Plant-Microbe Interactions, 2022
In Xanthomonas spp., the biosynthesis of the yellow pigment xanthomonadin and fatty acids originates in the type II polyketide synthase (PKS II) and fatty acid synthase (FAS) pathways, respectively. The acyl carrier protein (ACP) is the central component
Yi-Cai Chen, Zhe Hu, Wen-Bin Zhang, Yu Yin, Can-Yao Zhong, Wan-Ying Mo, Yong-Hong Yu, Jin-Cheng Ma, Hai-Hong Wang   +8 more
doaj   +1 more source

Subcellular localization of the mosquito sterol carrier protein-2 and sterol carrier protein-x [PDF]

Journal of Lipid Research, 2004
Subcellular distribution of Aedes aegypti sterol carrier protein-2 (AeSCP-2) and AeSCP-x was studied using electron microscopy. In both cultured A. aegypti cells and in the larval midgut, AeSCP-2 was detected mostly in the cytosol, with some labeling mitochondria and nucleus, but not in membranous vesicles. The widespread distribution of AeSCP-2 in the
Randall J. Massey, Que Lan
openaire   +4 more sources

Recent Advances in Bioconjugate Vaccine Development

Vaccines
Glycoconjugate vaccines, consisting of a protein component covalently linked to a glycan antigen, have led to a significant reduction in the global occurrence of bacterial meningitis and pneumonia. They provide robust, lasting immunity in all age groups.
Brendan W. Wren, Catherine L. Hall, Vanessa S. Terra, Mark A. Harrison, Elizabeth Atkins, Fauzy Nasher, Ian J. Passmore   +6 more
doaj   +1 more source

Immunization Effects of a Novel α-Synuclein-Based Peptide Epitope Vaccine in Parkinson’s Disease-Associated Pathology

Vaccines, 2023
Parkinson’s disease (PD) is a chronic neurodegenerative disease that affects the central nervous system, specifically the motor system. It is mainly caused by the loss of dopamine due to the accumulation of α-synuclein (α-syn) protein in the striatum and
Jun Sung Park, Riaz Ahmad, Kyonghwan Choe, Min Hwa Kang, Tae Ju Park, Myeong Ok Kim   +5 more
doaj   +1 more source

Acyl Carrier Protein Cyanylation Delivers a Ketoacyl Synthase–Carrier Protein Cross-Link [PDF]

Biochemistry, 2017
Acyl carrier proteins (ACPs) are central hubs in polyketide and fatty acid biosynthetic pathways, but the fast motions of the ACP's phosphopantetheine (Ppant) arm make its conformational dynamics difficult to capture using traditional spectroscopic approaches.
Grace A. R. Thiele, Connie P. Friedman, Kathleen J. S. Tsai, Joris Beld, Casey H. Londergan, Louise K. Charkoudian   +5 more
openaire   +3 more sources

Hydroxyethyl cellulose matrix applied to serial crystallography

Scientific Reports, 2017
Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the ...
Michihiro Sugahara, Takanori Nakane, Tetsuya Masuda, Mamoru Suzuki, Shigeyuki Inoue, Changyong Song, Rie Tanaka, Toru Nakatsu, Eiichi Mizohata, Fumiaki Yumoto, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, Eriko Nango, So Iwata   +18 more
doaj   +1 more source

An Overview on Spray-Drying of Protein-Loaded Polymeric Nanoparticles for Dry Powder Inhalation

Pharmaceutics, 2020
The delivery of therapeutic proteins remains a challenge, despite recent technological advances. While the delivery of proteins to the lungs is the gold standard for topical and systemic therapy through the lungs, the issue still exists.
Tânia Marante, Cláudia Viegas, Inês Duarte, Ana S. Macedo, Pedro Fonte   +4 more
doaj   +1 more source

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source

Tracking carrier protein motions with Raman spectroscopy

Nature Communications, 2019
Acyl carrier proteins (ACPs), a universal and highly conserved carrier of acyl intermediates during fatty acid and polyketide synthesis, are difficult to visualise.
Samuel C. Epstein, Adam R. Huff, Emily S. Winesett, Casey H. Londergan, Louise K. Charkoudian   +4 more
doaj   +1 more source