Results 141 to 150 of about 7,205 (191)

CRISPR-Cas systems for plant virus management: detection, surveillance, and host resistance. [PDF]

Front Plant Sci
Kumar S, Singh RM, Gadhave KR.
europepmc   +1 more source

A CRISPR/Cas12a-based reverse transcription multiple cross displacement amplification technique for sensitive, rapid, and highly specific detection of <i>human enterovirus A71</i> in clinical application. [PDF]

Front Microbiol
Zhang X, Ye R, Liang Q, Cao Y, Liu M, Wei F, Zhu Y, Yuan Y, Guo G, Wang Y.   +9 more
europepmc   +1 more source

A temporally controlled isothermal amplification-CRISPR/Cas12a platform for the rapid detection of monkeypox virus. [PDF]

Virol J
Wei Z, Xu X, Qiao Q, Guo J, Wu T, Rong H, Ning S, Zhu X, Zhao K, Ke J, He L, Chi Y, Ge Y, Cui L, Min X.   +14 more
europepmc   +1 more source

Targeted gene editing of <i>PCCA</i> pseudoexon using CRISPR-Cas12a for potential therapy in propionic acidemia. [PDF]

Mol Ther Nucleic Acids
Álvarez M, Del Álamo JV, Richard E, Desviat LR.   +3 more
europepmc   +1 more source

RAPID: evaluation of Cas12a protospacer nicking and chimeric reporters for PAM-independent RNA and DNA diagnostics. [PDF]

Nucleic Acids Res
Iwe IA, Liu FX, Corsano A, da Silva SJR, Doucet J, Singh S, Lamothe G, Zayani R, Nguyen J, Matthews Q, Vigar JRJ, Bayat P, Simchi M, Bozovicar K, Charania M, Panfilov S, Kelly P, Cai R, Hubbard BP, Li X, Mazzulli T, Tremblay JP, Zhao Y, Green AA, Li Z, Yao S, Pardee K.   +26 more
europepmc   +1 more source

Hybridization chain reaction-assisted CRISPR/Cas12a strategy for rapid and visual detection of <i>Haemophilus influenzae</i>. [PDF]

Front Cell Infect Microbiol
Ma C, Zhang J, Jiang Y, Li X.
europepmc   +1 more source

Some of the next articles are maybe not open access.

Related searches:

Improving the efficiency of CRISPR-Cas12a-based genome editing with site-specific covalent Cas12a-crRNA conjugates

Molecular Cell, 2021
The CRISPR-Cas12a system shows unique features compared with widely used Cas9, making it an attractive and potentially more precise alternative. However, the adoption of this system has been hindered by its relatively low editing efficiency. Guided by physical chemical principles, we covalently conjugated 5' terminal modified CRISPR RNA (crRNA) to a ...
Tao Liu, Xinyu Ling, Yujia Huang
exaly   +3 more sources

CRISPR-Cas12a-Assisted Recombineering in Bacteria. [PDF]

Applied and Environmental Microbiology, 2017
ABSTRACT Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a (Cpf1) has emerged as an effective genome editing tool in many organisms. Here, we developed and optimized a CRISPR-Cas12a-assisted recombineering system to facilitate genetic manipulation in bacteria.
Yi-Cheng Sun, Hai-Qin Yan, Gai-Xian Ren
exaly   +3 more sources