Results 161 to 170 of about 7,205 (191)

Immunity to CRISPR Cas9 and Cas12a therapeutics

WIREs Systems Biology and Medicine, 2017
Genome‐editing therapeutics are poised to treat human diseases. As we enter clinical trials with the most promising CRISPR‐Cas9 and CRISPR‐Cas12a (Cpf1) modalities, the risks associated with administering these foreign biomolecules into human patients become increasingly salient.
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Feasibility of implementing RPA coupled with CRISPR-Cas12a (RPA-Cas12a) for Hepatozoon canis detection in dogs

Veterinary Parasitology
Hepatozoonosis, caused by the protozoan Hepatozoon canis, is a prevalent blood disease affecting owned and stray dogs and cats. The prevalence of these parasites among companion animals in Thailand remains poorly understood. Diagnosing the old-world form of the disease is challenging due to the wide range of nonspecific clinical signs and the reliance ...
Suphaporn, Paenkaew, Anocha, Poommouang, Waranee, Pradit, Siriwadee, Chomdej, Korakot, Nganvongpanit, Puntita, Siengdee, Kittisak, Buddhachat   +6 more
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Metagenome-Derived CRISPR-Cas12a Mining and Characterization

The CRISPR Journal
The advent of clustered regularly interspaced short palindromic repeats (CRISPR)-based technologies has revolutionized genome editing, with continued interest in expanding the CRISPR-associated proteins (Cas) toolbox with diverse, efficient, and specific effectors.
Kalani Gast, Sydney Baker, Adair L. Borges, Stephanie Ward, Jillian F. Banfield, Rodolphe Barrangou   +5 more
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CRISPR-Cas12a/Cas13a in cancer molecular diagnosis

Clinica Chimica Acta
Cancer remains a leading cause of global mortality, with early diagnosis being pivotal for improving treatment outcomes. Traditional tissue biopsy is limited by its invasiveness, inability to capture tumor heterogeneity, and failure to support dynamic monitoring.
Juncheng, Lin, Yuyuan, Wang, Biyun, Zeng, Zhibing, Chen, Xiaocong, Lin, Tao, Zeng   +5 more
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Cas12a is competitive for gene editing in the malaria parasites

Microbial Pathogenesis
Malaria, caused by the Plasmodium parasites, has always been one of the worst infectious diseases that threaten human health, making it necessary for us to study the genetic function and physiological mechanisms of Plasmodium parasites from the molecular level to find more effective ways of addressing the increasingly pressing threat.
Shijie Yang, Yiming Wei, Elvis Quansah, Ziyu Zhang, Weiran Da, Bingjie Wang, Kaige Wang, Danhong Sun, Zhiyong Tao, Chao Zhang   +9 more
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RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification

Food Chemistry, 2021
Jinbin Wang, Xueming Tang
exaly  

Hs1Cas12a and Ev1Cas12a confer efficient genome editing in plants

Frontiers in Genome Editing, 2023
Yiping Qi, Yingxiao Zhang
exaly  

The development of a fluorescence/colorimetric biosensor based on the cleavage activity of CRISPR-Cas12a for the detection of non-nucleic acid targets

Journal of Hazardous Materials, 2023
Yu Wang, Huanying Zhou, Yuan Peng
exaly  

Framework-Hotspot Enhanced Trans Cleavage of CRISPR-Cas12a for Clinical Samples Detection

Angewandte Chemie - International Edition, 2023
Dayong Yang, Qianyun Tang, Fengqin Li
exaly  

CAS12A NICKASES

2023
VAN DER OOST JOHN, VILLEGAS WARREN RICARDO, LUTEIJN MAARTJE JANNEKE, STAALS RAYMOND HUBERT JOSÈPHE, WU WEN YING, DE VLEESSCHAUWER DAVID, D'HALLUIN KATELIJN, MEULEWAETER FRANK   +7 more
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