Results 151 to 160 of about 7,205 (191)

Multiplexed Genome Engineering with Cas12a

2021
Genome engineering technologies based on CRISPR-Cas systems are fueling efforts to study genotype-phenotype relationships in a high-throughput and multiplexed fashion. While many genome engineering technologies exist and provide a means to efficiently manipulate one or a few genes in a singular context-knockout, inhibition, or activation in a ...
Niels R, Weisbach, Ab, Meijs, Randall J, Platt   +2 more
openaire   +2 more sources

Mini crRNA-mediated CRISPR/Cas12a system (MCM-CRISPR/Cas12a) and its application in RNA detection

Talanta, 2023
Some non-coding RNAs are abnormally expressed during the occurrence and development of diseases, so it is necessary to develop analytical methods that can specifically and sensitively detect them. In typical CRISPR/Cas12a system, a complete crRNA that can recognize single-stranded or double-stranded DNA is necessary to activate its trans-cleavage ...
Xiaolong, Chen, Chaowang, Huang, Jing, Zhang, Qiao, Hu, Dan, Wang, Qianyi, You, Yawen, Guo, Huaping, Chen, Jing, Xu, Mingdong, Hu   +9 more
openaire   +2 more sources

A method for the quantitative detection of Cas12a ribonucleoproteins

Chemical Communications, 2020
We developed a method for the rapid and quantitative detection of the effective Cas12a RNPs by the combined use of anti-CRISPR protein AcrVA1 and stem-loop RT-qPCR, achieving a limit of detection (LOD) of 1 fM.
Jie Qiao, Siyu Lin, Wenli Sun, Lixin Ma, Yi Liu   +4 more
openaire   +2 more sources

CRISPR‐Cas12a System for Biosensing and Gene Regulation

Chemistry – An Asian Journal, 2021
AbstractClustered regularly interspaced short palindromic repeats (CRISPR) is a promising technology in the biological world. As one of the CRISPR‐associated (Cas) proteins, Cas12a is an RNA‐guided nuclease in the type V CRISPR‐Cas system, which has been a robust tool for gene editing.
Yuyan Shi, Xiaoyi Fu, Yao Yin, Fangqi Peng, Xia Yin, Guoliang Ke, Xiaobing Zhang   +6 more
openaire   +2 more sources

Optimized protocols for the characterization of Cas12a activities

2023
The CRISPR-associated (Cas) Cas12a is the effector protein for type V-A CRISPR systems. Cas12a is a sequence-specific endonuclease that targets and cleaves DNA containing a cognate short signature motif, called the protospacer adjacent motif (PAM), flanked by a 20 nucleotide (nt) segment that is complementary to the "guide" region of its CRISPR RNA ...
Lindsie, Martin, Saadi, Rostami, Rakhi, Rajan   +2 more
openaire   +2 more sources

Application of CRISPR/Cas12a in the rapid detection of pathogens

Clinica Chimica Acta, 2023
The combination of clustered regularly interspaced short palindromic repeats (CRISPR) and its associated Cas protein is an effective gene-editing instrument. Among them, the CRISPR-Cas12a system forms a DNA-cleavage-capable complex with crRNA and exerts its trans-cleavage activity by recognising the PAM site on the target pathogen's gene.
Yiheng Wang, Tianmu Yang, Guifang Liu, Longfei Xie, Jianying Guo, Wenguang Xiong   +5 more
openaire   +2 more sources

การพัฒนาเทคนิคการตรวจหาเชื้อไวรัสไข้หวัดใหญ่และเชื้อไวรัสโคโรนาสายพันธุ์ใหม่โดยใช้เทคโนโลยี CRISPR-Cas12a

2023
ในงานวิจัยนี้ ได้ประยุกต์ใช้เทคนิค CRISPR-Cas12a ซึ่งเป็นเทคนิคที่ง่าย รวดเร็ว และประหยัดค่าใช้จ่าย ในการตรวจหาเชื้อไวรัสไข้หวัดใหญ่ และเชื้อไวรัสโคโรนาสายพันธุ์ใหม่ จึงเหมาะสำหรับการตรวจคัดกรองผู้ป่วยโรคไข้หวัดใหญ่ และ โควิด-19 ในบริเวณที่มีอุปกรณ์ทางการแพทย์ที่จำกัด ผลการทดสอบพบว่า การใช้เทคนิค RT-RPA ร่วมกับ CRISPR-Cas12a ตรวจหาเชื้อไวรัสโคโรนาสายพ ...
openaire   +1 more source

Rapid and sensitive exosome detection with CRISPR/Cas12a

Analytical and Bioanalytical Chemistry, 2020
Numerous studies have shown that exosomes are closely related to the pathogenesis of various diseases, especially cancers. Therefore, a rapid and sensitive method for exosome detection will be of great importance for the diagnosis and prognosis of diseases.
Xianxian, Zhao, Wenqing, Zhang, Xiaopei, Qiu, Qiang, Mei, Yang, Luo, Weiling, Fu   +5 more
openaire   +2 more sources

Programmable Aptasensor for Regulating CRISPR/Cas12a Activity

ACS Sensors, 2023
CRISPR-mediated aptasensors have gained prevalence for detecting non-nucleic acid targets. However, there is an urgent need to develop an easily customizable design to improve the signal-to-noise ratio, enhance universality, and expand the detection range. In this article, we report a CRISPR-mediated programmable aptasensor (CPAS) platform.
Yao Yu, Qiaoyu Li, Wen Shi, Yuxin Yang, Hongpeng He, Junbiao Dai, Guobin Mao, Yingxin Ma   +7 more
openaire   +2 more sources

Simultaneous detection of CaMV35S and T-nos utilizing CRISPR/Cas12a and Cas13a with multiplex-PCR (MPT-Cas12a/13a)

Chemical Communications, 2022
MPT-Cas12a/13a for a specific and simultaneous assay of CaMV35S and T-nos based on CRISPR/Cas12a and Cas13a with different fluorescence signals.
Gaihua Cao, Jiangbo Dong, Xiaolong Chen, Peng Lu, Yifan Xiong, Lan Peng, Jiawei Li, Danqun Huo, Changjun Hou   +8 more
openaire   +2 more sources