Cell Reports, 2020 Summary: A key limitation of the widely used CRISPR enzyme S. pyogenes Cas9 is the strict requirement of an NGG protospacer-adjacent motif (PAM) at the target site. This constraint can be limiting for genome editing applications that require precise Cas9
Mateusz Legut +6 more
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Shortening the Half-Life of Cas9 Maintains Its Gene Editing Ability and Reduces Neuronal Toxicity
Cell Reports, 2018 Summary: Virus-mediated expression of CRISPR/Cas9 is commonly used for genome editing in animal brains to model or treat neurological diseases, but the potential neurotoxicity of overexpressing bacterial Cas9 in the mammalian brain remains unknown ...
Su Yang, Shihua Li, Xiao-Jiang Li
doaj
Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease [PDF]
, 2013 Wataru Fujii +3 more
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AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target Cleavage
Cell Reports, 2019 Summary: CRISPR-Cas9 is an adaptive immune system for prokaryotes to defend against invasive genetic elements such as phages and has been used as a powerful tool for genome editing and modulation.
Guoxu Song +6 more
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Generating rats with conditional alleles using CRISPR/Cas9 [PDF]
, 2013 Yuanwu Ma +8 more
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Production of CRISPR/Cas9-Mediated Self-Cleaving Helper-Dependent Adenoviruses
Molecular Therapy: Methods & Clinical Development, 2019 Prolonged expression of CRISPR/Cas9 raises concerns about off-target cleavage, cytotoxicity, and immune responses. To address these issues, we have developed a system to produce helper-dependent adenoviruses that express CRISPR/Cas9 to direct cleavage of
Donna J. Palmer +2 more
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A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes [PDF]
, 2013 Ming Ma +3 more
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Truncated gRNA reduces CRISPR/Cas9-mediated off-target rate for MSTN gene knockout in bovines
Journal of Integrative Agriculture, 2019 The CRISPR/Cas9 mediates efficient gene editing but has off-target effects inconducive to animal breeding. In this study, the efficacy of CRISPR/Cas9 vectors containing different lengths of gRNA in reduction of the off-target phenomenon in the bovine ...
Zheng-wei ZHOU +7 more
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In vivo evaluation of guide-free Cas9-induced safety risks in a pig model
Signal Transduction and Targeted TherapyThe CRISPR/Cas9 system has shown great potential for treating human genetic diseases through gene therapy. However, there are concerns about the safety of this system, specifically related to the use of guide-free Cas9.
Weikai Ge +14 more
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Representing Information on DNA using Patterns Induced by Enzymatic Labeling [PDF]
arXivEnzymatic DNA labeling is a powerful tool with applications in biochemistry, molecular biology, biotechnology, medical science, and genomic research. This paper contributes to the evolving field of DNA-based data storage by presenting a formal framework for modeling DNA labeling in strings, specifically tailored for data storage purposes.
arxiv