Results 101 to 110 of about 251,083 (336)

CRISPR-Cas9-mediated functional dissection of 3'-UTRs. [PDF]

, 2017
Many studies using reporter assays have demonstrated that 3' untranslated regions (3'-UTRs) regulate gene expression by controlling mRNA stability and translation.
Ahituv, Nadav, Biton, Anne, Erle, David J, Siegel, David, Tonqueze, Olivier Le, Zaitlen, Noah, Zhao, Wenxue   +6 more
core   +1 more source

DNA‑Directed Assembly of Photonic Nanomaterials for Diagnostic and Therapeutic Applications

Advanced Materials, EarlyView.
DNA‐directed assembly offers a powerful strategy for constructing structured photonic nanomaterials with precise spatial control. This review provides a comprehensive overview of recent advancements in DNA‐assembled photonic nanomaterials for diagnostics and therapeutics, highlighting key design principles, functionalization strategies, and optical ...
Longjiang Ding, Bing Liu, Andreas Peil, Sisi Fan, Jie Chao, Na Liu   +5 more
wiley   +1 more source

CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis [PDF]

, 2016
Retinoblastoma is a pediatric eye tumor in which bi-allelic inactivation of the Retinoblastoma 1 (RB1) gene is the initiating genetic lesion. Although recently curative rates of retinoblastoma have increased, there are at this time no molecular targeted ...
Boel, Annekatrien, Colpaert, Robin, Creytens, David, Deforce, Dieter, Dimitrakopoulou, Dionysia, Haustraete, Jurgen, L, J, Lepez, Trees, Naert, Thomas, Steyaert, Wouter, Van Nieuwenhuysen, Tom, Vleminckx, Kris, Willaert, Andy   +12 more
core   +2 more sources

Engineering CRISPR System‐Based Bacterial Outer Membrane Vesicle Potentiates T Cell Immunity for Enhanced Cancer Immunotherapy

Advanced Materials, EarlyView.
This study develops a multifunctional CRISPR‐dCas9‐based OMV platform termed OMV‐C9I12, which facilitates the coexpression of CXCL9 and IL‐12 within tumor cells. This platform enhances T cell recruitment and activation, synergizes with anti‐PD‐1/PD‐L1 immunotherapy, and amplifies antitumor T cell immunity.
Hongjin Wang, Hengji Zhan, Bolin Pan, Leli Zeng, Zehua Chen, Sen Liu, Qiang Zhang, Xuwei Hong, Junlin Lu, Xinrou Lin, Xiao Zhao, Jiajian Lai, Kaiwen Jie, Ye Li, Jianmei Zhong, Shengmeng Peng, Siting Chen, Changhao Chen, Wenlong Zhong, Shaoxu Wu, Yihang Pan, Tianxin Lin, Xu Chen   +22 more
wiley   +1 more source

Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus

Fungal Biology and Biotechnology, 2018
Background CRISPR/Cas9-based genome editing is quickly becoming a powerful tool within the field of fungal genetics. Adaptation of CRISPR/Cas9 systems are allowing for rapid and highly efficient gene targeting within fungi.
Qusai Al Abdallah, Ana Camila Oliveira Souza, Adela Martin-Vicente, Wenbo Ge, Jarrod R. Fortwendel   +4 more
doaj   +1 more source

CRISPR-TSKO : a technique for efficient mutagenesis in specific cell types, tissues, or organs in Arabidopsis [PDF]

, 2019
Detailed functional analyses of many fundamentally important plant genes via conventional loss-of-function approaches are impeded by the severe pleiotropic phenotypes resulting from these losses.
Andrade Buono, Rafael, Beeckman, Tom, Decaestecker, Ward, Jacobs, Thomas, Jourquin, Joris, Karimi, Mansour, Nowack, Moritz, Pfeiffer, Marie, Van Isterdael, Gert, Vangheluwe, Nick   +9 more
core   +1 more source

Harnessing Photo‐Energy Conversion in Nanomaterials for Precision Theranostics

Advanced Materials, EarlyView.
Harnessing photo‐energy conversion in nanomaterials enables precision theranostics through light‐driven mechanisms such as photoluminescence, photothermal, photoelectric, photoacoustic, photo‐triggered surface‐enhanced Raman scattering (SERS), and photodynamic processes. This review explores six fundamental principles of photo‐energy conversion, recent
Jingyu Shi, Yadi Fan, Qin Zhang, Yingying Huang, Mo Yang   +4 more
wiley   +1 more source

A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells

Molecular Therapy: Nucleic Acids, 2022
The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed.
Seung Bum Park, Takuro Uchida, Samantha Tilson, Zongyi Hu, Christopher D. Ma, Madeleine Leek, Michael Eichner, So Gun Hong, T. Jake Liang   +8 more
doaj  

CRISPR Cas9 in Pancreatic Cancer Research

Frontiers in Cell and Developmental Biology, 2019
Pancreatic cancer is now becoming a common cause of cancer death with no significant change in patient survival over the last 10 years. The main treatment options for pancreatic cancer patients are surgery, radiation therapy and chemotherapy, but there ...
Hai Yang, Peter Bailey, Christian Pilarsky   +2 more
doaj   +1 more source

CRISPR-Cas9 Gene Editing of Hematopoietic Stem Cells from Patients with Friedreich's Ataxia. [PDF]

, 2020
Friedreich's ataxia (FRDA) is an autosomal recessive neurodegenerative disorder caused by expansion of GAA repeats in intron 1 of the frataxin (FXN) gene, leading to significant decreased expression of frataxin, a mitochondrial iron-binding protein.
Cherqui, Stephanie, Haquang, Joseph H, Luebeck, Jens, Mali, Prashant, Rainaldi, Joseph N, Rocca, Celine J, Sharma, Jay, Shi, Yanmeng   +7 more
core