Results 101 to 110 of about 278,076 (329)
Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements. [PDF]
, 2019 Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements in the non-coding genome, but off-target effects in these experiments have not been systematically evaluated.
Aradhana +20 more
core
Spontaneous Embedding of DNA Mismatches Within the RNA:DNA Hybrid of CRISPR-Cas9. [PDF]
, 2020 CRISPR-Cas9 is the forefront technology for editing the genome. In this system, the Cas9 protein is programmed with guide RNAs to process DNA sequences that match the guide RNA forming an RNA:DNA hybrid structure.
Hsu, Rohaine V +5 more
core
Integrate CRISPR/Cas9 for protein expression of HLA-B*38:68Q via precise gene editing. [PDF]
, 2019 The determination of null- or low-expressed HLA alleles is clinically relevant in both hematopoietic stem cell transplantation and solid organ transplantation.
Reed, Elaine F +2 more
core +1 more source
A Human Neural Tube Model Using 4D Self‐Folding Smart Scaffolds
Advanced Healthcare Materials, EarlyView.Induced pluripotent stem cells (iPSCs) exhibit features comparable to the inner cell mass of the human embryo. iPSCs are applied to a novel self‐folding 4D‐Neural Tube (4D‐NT) structure that mimics the neurulation process. This 4D‐NT model recapitulates early events of human neural development and represents a platform to explore neurodevelopmental ...
Claudia Dell'Amico +8 more
wiley +1 more source
Gene editing restores dystrophin expression in a canine model of Duchenne muscular dystrophy [PDF]
, 2018 Mutations in the gene encoding dystrophin, a protein that maintains muscle integrity and function, cause Duchenne muscular dystrophy (DMD). The deltaE50-MD dog model of DMD harbors a mutation corresponding to a mutational “hotspot” in the human DMD gene.
Amoasii, L +12 more
core +2 more sources
Advanced Healthcare Materials, EarlyView.This study presents the first human neural organoid culture model capable of rapidly exhibiting long‐distance neural network propagation, thus delivering a system to experimentally investigate large‐scale communication during normal and diseased states.
Megh Dipak Patel +6 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.We present a strategy to enhance magnetic hyperthermia therapy by modulating nanoparticle–cell interactions. Antibody‐functionalized magnetic nanoparticles targeting the low‐internalizing CCR9 receptor enable spatially controlled membrane anchoring, reducing aggregation and maximizing heat generation under alternating magnetic fields.
David Egea‐Benavente +5 more
wiley +1 more source
CRISPR-Cas revolution in Agriculture: From precision genome editing to sustainable crop improvement
Current Innovation in Agriculture ScienceThe CRISPR-Cas system has emerged as a transformative tool in agricultural biotechnology, revolutionizing the landscape of crop improvement. This review paper explores the multifaceted applications of CRISPR technology in agriculture, from its ...
Anantha Rama A
doaj
Advanced Healthcare Materials, EarlyView.This review explores the evolving role of microneedle systems in psoriasis management, highlighting their potential for enhanced drug delivery, diagnosis, and disease monitoring. It also discusses unmet clinical needs for psoriasis management and technical challenges, while outlining strategic directions to advance microneedle integration into routine ...
Fatma Moawad +3 more
wiley +1 more source
CRISPR/Cas9, a universal tool for genomic engineering
Вавиловский журнал генетики и селекции, 2016 The CRISPR/Cas9 system was initially described as an element of archeal and bacterial immunity, but gained much attention recently for its outstanding ability to be programmed to target any genomic loci through a short 20-nucleotide sgRNA region. Here we
A. V. Smirnov +3 more
doaj +1 more source