Results 131 to 140 of about 251,083 (336)

Generating rats with conditional alleles using CRISPR/Cas9 [PDF]

, 2013
Yuanwu Ma, Xu Zhang, Bin Shen, Yingdong Lu, Wei Chen, Jing Ma, Lin Bai, Xingxu Huang, Lianfeng Zhang   +8 more
openalex   +1 more source

Rational Design of Inner Ear Drug Delivery Systems

Advanced Science, EarlyView.
Hearing loss is a common disease affecting many people, and inner ear lesions are one of the most important causes. This review focuses on the treatment of inner ear hearing loss by drug delivery systems. It includes the current methods and technologies developed, and it predicts possible directions.
Xiayidan Maimaitikelimu, Zhiyan Xuan, Haoyu Ren, Keng Chen, Hui Zhang, Huan Wang   +5 more
wiley   +1 more source

Shortening the Half-Life of Cas9 Maintains Its Gene Editing Ability and Reduces Neuronal Toxicity

Cell Reports, 2018
Summary: Virus-mediated expression of CRISPR/Cas9 is commonly used for genome editing in animal brains to model or treat neurological diseases, but the potential neurotoxicity of overexpressing bacterial Cas9 in the mammalian brain remains unknown ...
Su Yang, Shihua Li, Xiao-Jiang Li
doaj  

A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes [PDF]

, 2013
Ming Ma, Adam Yongxin Ye, Weiguo Zheng, Lei Kong   +3 more
openalex   +1 more source

CRISPR/Cas9-mediated mutagenesis of CAROTENOID CLEAVAGE DIOXYGENASE 8 in tomato provides resistance against the parasitic weed Phelipanche aegyptiaca. [PDF]

, 2019
Broomrapes (Phelipanche aegyptiaca and Orobanche spp.) are obligate plant parasites that cause extreme damage to crop plants. The parasite seeds have strict requirements for germination, involving preconditioning and exposure to specific chemicals ...
Aly, Radi, Bari, Vinay Kumar, Britt, Anne, Gal-On, Amit, Kheredin, Sally Marzouk, Nassar, Jackline Abu, Ron, Mily, Steele, Daniel, Yoder, John   +8 more
core  

IncRNA‐ZFAS1, an Emerging Gate‐Keeper in DNA Damage‐Dependent Transcriptional Regulation

Advanced Science, EarlyView.
LncZFAS1 plays a crucial role during DNA damage response in mammalian cells. Loss of lncZFAS1 results in deficient DNA lesion removal and reduced cell viability. Mechanistically, lncZFAS1 modulates RNAPII phosphorylation and transcription and thereby promotes both GG‐NER and TC‐NER upon UV damage.
Jiena Liu, Qing Lu, Zixuan Fan, Jiahui Lin, Nan He, Xin Zhang, Zhaoya Han, Tingting Zhu, Zhenzhen Wu, Yingying Xu, Yuming Wang   +10 more
wiley   +1 more source

Development and testing of a versatile genome editing application reporter (V-GEAR) system

Molecular Therapy: Methods & Clinical Development
CRISPR-Cas9 and novel cas fusion proteins leveraging specific DNA targeting ability combined with deaminases or reverse transcriptases have revolutionized genome editing.
Evan W. Kleinboehl, Kanut Laoharawee, Walker S. Lahr, Jacob D. Jensen, Joseph J. Peterson, Jason B. Bell, Beau R. Webber, Branden S. Moriarity   +7 more
doaj  

AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target Cleavage

Cell Reports, 2019
Summary: CRISPR-Cas9 is an adaptive immune system for prokaryotes to defend against invasive genetic elements such as phages and has been used as a powerful tool for genome editing and modulation.
Guoxu Song, Fei Zhang, Xuewen Zhang, Xing Gao, Xiaoxiao Zhu, Dongdong Fan, Yong Tian   +6 more
doaj  

Integrating Dense Genotyping with High‐Throughput Phenotyping Empowers the Genetic Dissection of Berry Quality and Resilience Traits in Grapevine

Advanced Science, EarlyView.
Researchers develop advanced tools to study grapevine traits like berry quality and stress resilience. A 200K SNP array and high‐throughput phenotyping enable the identification of loci linked to berry shape, sugar content, acidity, and cold tolerance. Functional validation of genes such as NAC08 reveals roles in cold tolerance.
Yuyu Zhang, Yongjian Wang, Michael Henke, Pablo Carbonell‐Bejerano, Zemin Wang, Pierre‐François Bert, Yi Wang, Huayang Li, Junhua Kong, Peige Fan, Zhanwu Dai, Zhenchang Liang   +11 more
wiley   +1 more source

Assessment of a Split Homing Based Gene Drive for Efficient Knockout of Multiple Genes

G3: Genes, Genomes, Genetics, 2020
Nikolay P. Kandul, Junru Liu, Anna Buchman, Valentino M. Gantz, Ethan Bier, Omar S. Akbari   +5 more
doaj   +1 more source