Results 161 to 170 of about 251,083 (336)
Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells [PDF]
, 2014 Yilan Zhang +8 more
openalex +1 more source
, 2018 A polynucleotide encoding a ThermoCas9protein from Geobacillus thermodenitrificans and a constitutive promoter are used to engineer eukaryotic cells, e.g. fungi, yeast or algae, so that the ThermoCas9 endonuclease is integrated and expressed from the genome of the cell.
van der Oost, J. +4 more
openaire +3 more sources
Forage Crop Research in the Modern Age
Advanced Science, EarlyView.Forage crops are increasingly vital to sustainable agriculture, yet urgent demands for transformative innovations create unprecedented opportunities and challenges. This review synthesizes the latest advances in forage research, spotlighting cutting‐edge breeding technologies and their transformative potential.
Qikun Liu +11 more
wiley +1 more source
CRISPR/Cas9 as a Mutagenic Factor
International Journal of Molecular SciencesThe discovery of the CRISPR/Cas9 microbial adaptive immune system has revolutionized the field of genetics, by greatly enhancing the capacity for genome editing. CRISPR/Cas9-based editing starts with DNA breaks (or other lesions) predominantly at target sites and, unfortunately, at off-target genome sites.
Andrey R. Shumega +5 more
openaire +2 more sources
Biosecurity Primitive: Polymerase X‐based Genetic Physical Unclonable Functions
Advanced Science, EarlyView.What if every cell line can carry an unforgeable genomic fingerprint—one that proves its origin, ensures its authenticity, and thwarts unauthorized duplication? Here, CRISPR‐induced DNA lesions paired with TdT‐mediated repair produce robust, unique, and unclonable genetic signatures—advancing the concept of molecular Physical Unclonable Functions (PUF)
Zikun Zhou +7 more
wiley +1 more source
Structural insights into Cas9 mismatch: promising for development of high-fidelity Cas9 variants
Signal Transduction and Targeted Therapy, 2022 Honghai Tang, Daqi Wang, Yilai Shu
doaj +1 more source
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins [PDF]
, 2014 Sojung Kim +4 more
openalex +1 more source
Advanced Science, EarlyView.This study highlights the role of connexin43 (Cx43) in mediating glia activation and neuroinflammation in glaucoma. Using Cx43 knockout mice, it is shown that macroglial cells‐derived Cx43 modulates microglial activation, proliferation, migration, and polarization.
Guoli Zhao +17 more
wiley +1 more source
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing
, 2013 Kevin M. Esvelt +5 more
openalex +2 more sources
Advanced Science, EarlyView.This study integrates dual‐reporter genetics, imaging flow cytometry, and single‐cell sequencing to characterize rare bihormonal cells in mouse and human pancreatic islets. Gcg⁺Ins⁺ cells resemble α‐cells rather than transitional states. Cross‐species gene network analysis refines islet cell taxonomy and reveals human‐specific δ‐cell subtypes ...
Xin‐Xin Yu +10 more
wiley +1 more source