Results 11 to 20 of about 152,507 (240)
Cas9 Functionally Opens Chromatin. [PDF]
PLoS ONE, 2016 Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler ...
Amira A Barkal +4 more
doaj +6 more sources
Orthogonal Cas9–Cas9 chimeras provide a versatile platform for genome editing [PDF]
Nature Communications, 2018 Therapeutic genome engineering relies on the development of reliable, robust and versatile tools. Here the authors develop Cas9-Cas9 chimeras with high target site activity that generate predictable deletions.
Mehmet Fatih Bolukbasi +8 more
doaj +4 more sources
CRISPR/CAS9 Technologies [PDF]
Journal of Bone and Mineral Research, 2017 ABSTRACT The Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) pathway is revolutionizing biological research. Modifications to this primitive prokaryotic immune system now enable scientists to efficiently edit DNA or modulate gene expression in living ...
Bart O, Williams, Matthew L, Warman
openaire +2 more sources
Current Opinion in Biotechnology, 2017 Ever since its discovery, Cas9 from Streptococcus pyogenes has revolutionized biology by enabling analysis and engineering of genomes with unprecedented precision and ease. To fine-tune on-target effects and to mitigate adverse effects caused by untimely and off-target action of Cas9, strategies have been developed to control its activity at the post ...
Richter, F. +5 more
openaire +5 more sources
Veterinarska stanica, 2021 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR- associated system (Cas) is a system that provides immunity to most prokaryoticorganisms against viral attacks and other foreign bodies. CRISPR systems consist of a scissor-like protein called Cas9 and a genetic GPS guide “The guide RNA”.
Mimoune, Nora +4 more
openaire +3 more sources
Adding to the CASeload: unwarranted p53 signaling induced by Cas9
Molecular & Cellular Oncology, 2020 We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines.
Veronica Rendo +2 more
doaj +1 more source
Creating a tobacco line with a weaker antifeedant property against colorado potato beetle
Биотехнология и селекция растений, 2020 Background. Genetic modification of plants is one of the promising strategies to increase their resistance to insect pests. The development of metabolic or RNA interference systems for plant protection requires appropriate models of host-insect ...
N. E. Kostina +10 more
doaj +1 more source
Zhongliu Fangzhi Yanjiu, 2020 Objective To observe the efficiency of Cas9 RNP technology for the preparation of B2M and PD-1 double genes knock-out primary human T lymphocytes (hereinafter referred to as T cells).
XU Ya +3 more
doaj +1 more source
Immunogenicity of Cas9 Protein [PDF]
Journal of Pharmaceutical Sciences, 2020 Clustered regularly interspaced short palindromic repeats (CRISPR) form the adaptive immune system in archaea and bacteria and have been modified for genome engineering in eukaryotic cells. CRISPR systems contain 2 components, a single-guide RNA, which is a short RNA composed of a 20 nucleotide sequence that targets specific sites in the genomic DNA ...
Mehta, Aditi, Merkel, Olivia M.
openaire +2 more sources
Computationally designed hyperactive Cas9 enzymes
Nature Communications, 2022 The ability to alter the genomes of living cells is key to understanding how genes influence the functions of organisms and will be critical to modify living systems for useful purposes. Here, the authors use computational design to discover Cas9 enzymes
Pascal D. Vos +10 more
doaj +1 more source