Results 11 to 20 of about 251,083 (336)
Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9. [PDF]
, 2016 The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5'-NGG-3' protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences.
Chen, Cheng +8 more
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Smash and DASH with Cas9 [PDF]
Genome Biology, 2016 DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.
Jay Shendure, Jay Shendure, Vijay Ramani
openaire +3 more sources
Archives of disease in childhood - Education & practice edition, 2016 Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology causing a major upheaval in biomedical research. It makes it possible to correct errors in the genome and turn on or off genes in cells and organisms quickly, cheaply and with relative ease.
Andrew J. King +3 more
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CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]
, 2020 The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I +2 more
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CRISPR/CAS9 Technologies [PDF]
Journal of Bone and Mineral Research, 2017 ABSTRACT The Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) pathway is revolutionizing biological research. Modifications to this primitive prokaryotic immune system now enable scientists to efficiently edit DNA or modulate gene expression in living ...
Matthew L. Warman, Bart O. Williams
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Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
Theranostics, 2021 CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years, however, the translation of this biotechnology into therapy has been hindered by efficient delivery of CRISPR/Cas9 materials into target cells. Direct delivery of CRISPR/Cas9 system as a ribonucleoprotein (RNP) complex consisting of Cas9 protein and single guide RNA ...
Jiangtao Shen +4 more
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Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]
, 2020 The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes +5 more
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G3: Genes, Genomes, Genetics, 2020 Neighboring sequences of a gene can influence its expression. In the phenomenon known as transcriptional interference, transcription at one region in the genome can repress transcription at a nearby region in cis. Transcriptional interference occurs at a
Victoria Jorgensen +12 more
doaj +1 more source
Prospects for using CRISPR-Cas9 system in the treatment of human viral diseases
Acta Biomedica Scientifica, 2023 The aim. To analyze the possibility of using the genetic mechanisms of CRISPR-Cas9 technology in the prevention and treatment of certain viral diseases.Materials and methods.
A. M. Ziganshin +4 more
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In Vitro Enzymology of Cas9 [PDF]
, 2014 Cas9 is a bacterial RNA-guided endonuclease that uses base pairing to recognize and cleave target DNAs with complementarity to the guide RNA. The programmable sequence specificity of Cas9 has been harnessed for genome editing and gene expression control in many organisms.
Carolin Anders, Martin Jinek
openaire +4 more sources