Results 11 to 20 of about 194,133 (208)

A genomic sequence of the type II-A clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system in Mycoplasma salivarium strain ATCC 29803

Journal of Oral Microbiology, 2022
Introduction Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems are RNA-mediated adaptive immune systems that actagainst invading genetic elements such as phages or plasmids.
Harumi Mizuki, Yu Shimoyama, Taichi Ishikawa, Minoru Sasaki   +3 more
doaj   +1 more source

Current applications and future perspective of CRISPR/Cas9 gene editing in cancer

Molecular Cancer, 2022
Clustered regularly interspaced short palindromic repeats (CRISPR) system provides adaptive immunity against plasmids and phages in prokaryotes. This system inspires the development of a powerful genome engineering tool, the CRISPR/CRISPR-associated ...
Si-wei Wang, Chao Gao, Yi-Min Zheng, Li Yi, Jia-Cheng Lu, Xiao-yong Huang, Jia-bin Cai, Peng-fei Zhang, Yuexin Cui, A. Ke   +9 more
semanticscholar   +1 more source

Prospects for using CRISPR-Cas9 system in the treatment of human viral diseases

Acta Biomedica Scientifica, 2023
The aim. To analyze the possibility of using the genetic mechanisms of CRISPR-Cas9 technology in the prevention and treatment of certain viral diseases.Materials and methods.
A. M. Ziganshin, A. R. Mulyukov, M. A. Omarov, V. A. Mudrov, R. Sh. Khalitova   +4 more
doaj   +1 more source

Tunable Transcriptional Interference at the Endogenous Alcohol Dehydrogenase Gene Locus in Drosophila melanogaster

G3: Genes, Genomes, Genetics, 2020
Neighboring sequences of a gene can influence its expression. In the phenomenon known as transcriptional interference, transcription at one region in the genome can repress transcription at a nearby region in cis. Transcriptional interference occurs at a
Victoria Jorgensen, Jingxun Chen, Helen Vander Wende, Devon E. Harris, Alicia McCarthy, Shane Breznak, Siu Wah Wong-Deyrup, Yuzhang Chen, Prashanth Rangan, Gloria Ann Brar, Eric M. Sawyer, Leon Y. Chan, Elçin Ünal   +12 more
doaj   +1 more source

Evaluating the feasibility of Cas9 overexpression in 3T3-L1 cells for generation of genetic knock-out adipocyte cell lines

Adipocyte, 2021
Cell lines recapitulating physiological processes can represent alternatives to animal or human studies. The 3T3-L1 cell line is used to mimic adipocyte function and differentiation. Since transfection of 3T3-L1 cells is difficult, we used a modified 3T3-
Tomás Suchý, Isabell Kaczmarek, Tomislav Maricic, Christian Zieschang, Torsten Schöneberg, Doreen Thor, Ines Liebscher   +6 more
doaj   +1 more source

Optimizing CRISPR Cas9 Genome Editing System: A Review

International Journal of Endorsing Health Science Research, 2017
CRISPR Cas9 is highly advanced genome editing technology extensively used for the modifications of genetic components in various sectors of living organisms. This technology has been adapted from the prokaryotic immune system, where it plays a vital role
Meen Bahadur Budhthoki, Puja sharma, Saima Khan, Saroj Singh, Fu Chao , Li Yong   +5 more
doaj   +1 more source

RNA-Guided Human Genome Engineering via Cas9

Science, 2013
Genome Editing Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targeted for cleavage by complementary CRISPR RNAs (crRNAs) bound
P. Mali, Luhan Yang, K. Esvelt, John D Aach, M. Guell, James E. DiCarlo, J. Norville, G. Church   +7 more
semanticscholar   +1 more source

The Involvement of the Cas9 Gene in Virulence of Campylobacter jejuni

Frontiers in Cellular and Infection Microbiology, 2018
Campylobacter jejuni is considered as the leading cause of gastroenteritis all over the world. This bacterium has the CRISPR–cas9 system, which is used as a gene editing technique in different organisms.
Muhammad A. B. Shabbir, Yanping Tang, Zihui Xu, Mingyue Lin, Guyue Cheng, Menghong Dai, Xu Wang, Zhengli Liu, Zonghui Yuan, Zonghui Yuan, Haihong Hao, Haihong Hao   +11 more
doaj   +1 more source

Preparation of B2M and PD-1 Efficiently Double Genes Knock-out Primary Human T Lymphocytes by Cas9 RNP Technology

Zhongliu Fangzhi Yanjiu, 2020
Objective To observe the efficiency of Cas9 RNP technology for the preparation of B2M and PD-1 double genes knock-out primary human T lymphocytes (hereinafter referred to as T cells).
XU Ya, FU Yang, ZHU Shicong, ZHANG Bicheng   +3 more
doaj   +1 more source

ChemSpaceAL: An Efficient Active Learning Methodology Applied to Protein-Specific Molecular Generation [PDF]

, 2023
The incredible capabilities of generative artificial intelligence models have inevitably led to their application in the domain of drug discovery. Within this domain, the vastness of chemical space motivates the development of more efficient methods for identifying regions with molecules that exhibit desired characteristics.
arxiv   +1 more source