Results 21 to 30 of about 251,083 (336)
Adipocyte, 2021 Cell lines recapitulating physiological processes can represent alternatives to animal or human studies. The 3T3-L1 cell line is used to mimic adipocyte function and differentiation. Since transfection of 3T3-L1 cells is difficult, we used a modified 3T3-
Tomás Suchý +6 more
doaj +1 more source
Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, Aedes aegypti. [PDF]
, 2017 The development of CRISPR/Cas9 technologies has dramatically increased the accessibility and efficiency of genome editing in many organisms. In general, in vivo germline expression of Cas9 results in substantially higher activity than embryonic injection.
Akbari, Omar S +5 more
core +1 more source
Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections. [PDF]
, 2018 The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein⁻Barr virus, cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus.
Chen, Yuan-Chuan +3 more
core +2 more sources
Identification and Evolution of Cas9 tracrRNAs [PDF]
The CRISPR Journal, 2020 AbstractCas9 trans-activating CRISPR RNAs (tracrRNAs) form distinct structures essential for target recognition and cleavage and dictate exchangeability between orthologous proteins. As non-coding RNAs that are often apart from the CRISPR array, their identification can be arduous.
Shane K. Dooley +4 more
openaire +4 more sources
Optimizing CRISPR Cas9 Genome Editing System: A Review
International Journal of Endorsing Health Science Research, 2017 CRISPR Cas9 is highly advanced genome editing technology extensively used for the modifications of genetic components in various sectors of living organisms. This technology has been adapted from the prokaryotic immune system, where it plays a vital role
Meen Bahadur Budhthoki +5 more
doaj +1 more source
Zhongliu Fangzhi Yanjiu, 2020 Objective To observe the efficiency of Cas9 RNP technology for the preparation of B2M and PD-1 double genes knock-out primary human T lymphocytes (hereinafter referred to as T cells).
XU Ya +3 more
doaj +1 more source
Improved genome editing in human cell lines using the CRISPR method [PDF]
, 2014 The Cas9/CRISPR system has become a popular choice for genome editing. In this system, binding of a single guide (sg) RNA to a cognate genomic sequence enables the Cas9 nuclease to induce a double-strand break at that locus.
AW Briggs +22 more
core +4 more sources
Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]
, 2020 Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A +5 more
core +3 more sources
The Involvement of the Cas9 Gene in Virulence of Campylobacter jejuni
Frontiers in Cellular and Infection Microbiology, 2018 Campylobacter jejuni is considered as the leading cause of gastroenteritis all over the world. This bacterium has the CRISPR–cas9 system, which is used as a gene editing technique in different organisms.
Muhammad A. B. Shabbir +11 more
doaj +1 more source
Adding to the CASeload: unwarranted p53 signaling induced by Cas9
Molecular & Cellular Oncology, 2020 We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines.
Veronica Rendo +2 more
doaj +1 more source