Results 21 to 30 of about 152,507 (240)
Delivering CRISPR: a review of the challenges and approaches
Drug Delivery, 2018 Gene therapy has long held promise to correct a variety of human diseases and defects. Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the mechanism of the CRISPR-based prokaryotic adaptive immune system (CRISPR ...
Christopher A. Lino +3 more
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Off- and on-target effects of genome editing in mouse embryos
The Journal of Reproduction and Development, 2018 Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome.
Shinya AYABE +2 more
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Genes and Diseases, 2022 The CRISPR/Cas9 system, originally derived from the prokaryotic adaptive immune system, has been developed as efficient genome editing tools. It enables precise gene manipulation on chromosomal DNA through the specific binding of programmable sgRNA to ...
Renke Tan +11 more
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Вавиловский журнал генетики и селекции, 2019 The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova +11 more
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Veterinarska stanica, 2021 CRISPR/Cas9 has become a powerful method for making changes to the genome of many organisms. First discovered in bacteria as part of an adaptive immune system, CRISPR/Cas9 and modified versions have found widespread use in genome engineering and in the activation or repression of gen expression.
Mimoune, Nora +5 more
openaire +2 more sources
Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity [PDF]
Science Advances, 2020 Fusing attenuated anti-CRISPR proteins to Cas9 improves specificity via kinetic insulation of ON- and OFF-target editing events.
Sabine Aschenbrenner +5 more
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Molecular Therapy: Nucleic Acids, 2022 The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed.
Seung Bum Park +8 more
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FEBS Letters, EarlyView.This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley +1 more source
Concerning RNA-guided gene drives for the alteration of wild populations
eLife, 2014 Gene drives may be capable of addressing ecological problems by altering entire populations of wild organisms, but their use has remained largely theoretical due to technical constraints. Here we consider the potential for RNA-guided gene drives based on
Kevin M Esvelt +3 more
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Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source