Results 21 to 30 of about 194,133 (208)

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

Nature Biotechnology, 2015
CRISPR-Cas9–based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off ...
John G Doench, Nicoló Fusi, Meagan E. Sullender, Mudra Hegde, Emma Vaimberg, Katherine F. Donovan, Ian Smith, Z. Tothova, Craig B. Wilen, R. Orchard, H. Virgin, J. Listgarten, D. Root   +12 more
semanticscholar   +1 more source

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Science, 2020
A PAMless base editor CRISPR-Cas DNA base editing typically requires a specific motif for targeting known as a protospacer-adjacent motif (PAM). This requirement limits the sequences within a genome that can be targeted. Walton et al. engineered specific
R. Walton, K. Christie, Madelynn N. Whittaker, B. Kleinstiver   +3 more
semanticscholar   +1 more source

Debating CRISPR/cas9 and Mitochondrial Donation: Continuity and Transition Performances at Scientific Conferences

Engaging Science, Technology, and Society, 2016
Conferences are important performative sites. Here we detail a UK science policy conference debating the novel biomedical techniques CRISPR/cas9 and mitochondrial donation.
Neil Stephens, Rebecca Dimond
doaj   +1 more source

Adding to the CASeload: unwarranted p53 signaling induced by Cas9

Molecular & Cellular Oncology, 2020
We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines.
Veronica Rendo, Oana M. Enache, Uri Ben-David   +2 more
doaj   +1 more source

Cas9 Functionally Opens Chromatin. [PDF]

PLoS ONE, 2016
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler ...
Amira A Barkal, Sharanya Srinivasan, Tatsunori Hashimoto, David K Gifford, Richard I Sherwood   +4 more
doaj   +1 more source

Structural basis for mismatch surveillance by CRISPR–Cas9

Nature, 2022
CRISPR–Cas9 as a programmable genome editing tool is hindered by off-target DNA cleavage1–4, and the underlying mechanisms by which Cas9 recognizes mismatches are poorly understood5–7. Although Cas9 variants with greater discrimination against mismatches
Jack P. K. Bravo, Mu-Sen Liu, Grace N. Hibshman, Tyler L. Dangerfield, Kyungseok Jung, R. S. McCool, K. Johnson, David W. Taylor   +7 more
semanticscholar   +1 more source

Creating a tobacco line with a weaker antifeedant property against colorado potato beetle

Биотехнология и селекция растений, 2020
Background. Genetic modification of plants is one of the promising strategies to increase their resistance to insect pests. The development of metabolic or RNA interference systems for plant protection requires appropriate models of host-insect ...
N. E. Kostina, A. V. Spaselnikova, A. A. Egorova, E. V. Kolosovskaya, D. V. Domrachev, A. V. Romanova, S. R. Tumanyan, S. Chamas, J. Kumlehn, I. M. Dubovskiy, S. V. Gerasimova   +10 more
doaj   +1 more source

Computationally designed hyperactive Cas9 enzymes

Nature Communications, 2022
The ability to alter the genomes of living cells is key to understanding how genes influence the functions of organisms and will be critical to modify living systems for useful purposes. Here, the authors use computational design to discover Cas9 enzymes
Pascal D. Vos, Giulia Rossetti, Jessica L. Mantegna, Stefan J. Siira, Andrianto P. Gandadireja, Mitchell Bruce, Samuel A. Raven, Olga Khersonsky, Sarel J. Fleishman, Aleksandra Filipovska, Oliver Rackham   +10 more
doaj   +1 more source

In vivo genome editing using Staphylococcus aureus Cas9

Nature, 2015
The RNA-guided endonuclease Cas9 has emerged as a versatile genome-editing platform. However, the size of the commonly used Cas9 from Streptococcus pyogenes (SpCas9) limits its utility for basic research and therapeutic applications that use the highly ...
F. Ran, Le Cong, Winston X. Yan, David A. Scott, J. Gootenberg, Andrea J. Kriz, Bernd Zetsche, Ophir Shalem, Xuebing Wu, K. Makarova, E. Koonin, P. Sharp, Feng Zhang   +12 more
semanticscholar   +1 more source

Engineering the next-generation of CAR T-cells with CRISPR-Cas9 gene editing

Molecular Cancer, 2022
Chimeric Antigen Receptor (CAR) T-cells represent a breakthrough in personalized cancer therapy. In this strategy, synthetic receptors comprised of antigen recognition, signaling, and costimulatory domains are used to reprogram T-cells to target tumor ...
Alexander J. Dimitri, F. Herbst, J. Fraietta   +2 more
semanticscholar   +1 more source