Results 71 to 80 of about 194,133 (208)
Suppression of HBV replication by the expression of nickase- and nuclease dead-Cas9
Scientific Reports, 2017 Complete removal of hepatitis B virus (HBV) DNA from nuclei is difficult by the current therapies. Recent reports have shown that a novel genome-editing tool using Cas9 with a single-guide RNA (sgRNA) system can cleave the HBV genome in vitro and in vivo.
Takeshi Kurihara +12 more
doaj +1 more source
Excision of Nucleopolyhedrovirus Form Transgenic Silkworm Using the CRISPR/Cas9 System
Frontiers in Microbiology, 2018 The CRISPR/Cas9-mediated genome engineering has been shown to efficiently suppress infection by disrupting genes of the pathogen. We recently constructed transgenic lines expressing CRISPR/Cas9 and the double sgRNA target Bombyx mori nucleopolyhedrovirus
Zhanqi Dong +10 more
doaj +1 more source
Nature Biotechnology, 2018 In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control differentiation of the two sexes.
Kyros Kyrou +7 more
semanticscholar +1 more source
CRISPR Cas9 in Pancreatic Cancer Research
Frontiers in Cell and Developmental Biology, 2019 Pancreatic cancer is now becoming a common cause of cancer death with no significant change in patient survival over the last 10 years. The main treatment options for pancreatic cancer patients are surgery, radiation therapy and chemotherapy, but there ...
Hai Yang +2 more
doaj +1 more source
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
Nature, 2015 Although CRISPR-Cas9 nucleases are widely used for genome editing, the range of sequences that Cas9 can recognize is constrained by the need for a specific protospacer adjacent motif (PAM). As a result, it can often be difficult to target double-stranded
B. Kleinstiver +15 more
semanticscholar +1 more source
Optimized Plasmid Construction Strategy for Cas9
Cellular Physiology and Biochemistry, 2018 Background/Aims: The target genome editing technology not only plays an important role in basic biology studies but also holds a great promise for potential clinical applications.
Jianyong Xu +5 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2022 The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed.
Seung Bum Park +8 more
doaj
Fungal Biology and Biotechnology, 2018 Background CRISPR/Cas9-based genome editing is quickly becoming a powerful tool within the field of fungal genetics. Adaptation of CRISPR/Cas9 systems are allowing for rapid and highly efficient gene targeting within fungi.
Qusai Al Abdallah +4 more
doaj +1 more source
Engineered CRISPR-Cas9 nuclease with expanded targeting space
Science, 2018 Expanding the targeting space of Cas9 CRISPR-Cas9 associates with a guide RNA to target and cleave a specific DNA site next to a protospacer adjacent motif (PAM).
H. Nishimasu +20 more
semanticscholar +1 more source
CRISPR/Cas9, a universal tool for genomic engineering
Вавиловский журнал генетики и селекции, 2016 The CRISPR/Cas9 system was initially described as an element of archeal and bacterial immunity, but gained much attention recently for its outstanding ability to be programmed to target any genomic loci through a short 20-nucleotide sgRNA region. Here we
A. V. Smirnov +3 more
doaj +1 more source