Results 71 to 80 of about 251,083 (336)
Genome editing technologies to fight infectious diseases [PDF]
Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa+2 more
core +1 more source
Boundaries of photosynthesis: adaptations of carbon fixation in extreme environments
Photosynthesis faces challenges from environmental extremes of temperature, pH, and salinity, limiting gas diffusion, modifying membrane fluidity, and destabilizing photochemical and biochemical reactions. Photosynthetic organisms have evolved unique adaptations overcoming these stresses and maintaining their photosynthetic activity.
Pere Aguiló‐Nicolau+3 more
wiley +1 more source
In CRISPR/Cas9 system, there are two critical parts: sgRNA sequence and Cas9 sequence. These two sequence could be cloned into separated plasmids or combined into one plasmids. Considering the limited utility of protoplast system, it’s also available to transfect preassembled complexes of purified Cas9 protein and synthesized sgRNA.
openaire +2 more sources
CRISPR/Cas9 has become a powerful method for making changes to the genome of many organisms. First discovered in bacteria as part of an adaptive immune system, CRISPR/Cas9 and modified versions have found widespread use in genome engineering and in the activation or repression of gen expression.
Mohamed Wail Bahouh+4 more
openaire +3 more sources
Development of 4T1 breast cancer mouse model system for preclinical carbonic anhydrase IX studies
Carbonic anhydrase IX (CAIX) is a well‐recognised therapeutic target and prognostic biomarker in cancer. We developed and characterised a robust murine breast cancer model system that is suitable for CAIX studies in vitro and in vivo—it comprises both CAIX‐positive and CAIX‐negative controls and provides a solid platform for the comprehensive ...
Zane Kalniņa+13 more
wiley +1 more source
The tsunami named CRISPR/Cas9 [PDF]
It is rare to witness a breakthrough technology that results in a multidimensional revolution in human health that also has impacts on animals and plants as well as biodiversity and the environment. The tsunami associated with the latest genome editing techniques is most likely not comparable to anything we have known thus far.
openaire +3 more sources
Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements. [PDF]
Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements in the non-coding genome, but off-target effects in these experiments have not been systematically evaluated.
Aradhana+20 more
core
Advances in Engineering the Fly Genome with the CRISPR-Cas System. [PDF]
Drosophila has long been a premier model for the development and application of cutting-edge genetic approaches. The CRISPR-Cas system now adds the ability to manipulate the genome with ease and precision, providing a rich toolbox to interrogate ...
Bier, Ethan+3 more
core +2 more sources
Mitochondria contain two mitoribosome rescue factors, ICT1 and MTRFR (C12orf65). ICT1 also functions as a mitoribosomal protein in mice and humans, and its loss is lethal. Although Mtrfr knockout mice could not be generated, knockout zebrafish lines for ict1 and mtrfr were established.
Nobukazu Nameki+11 more
wiley +1 more source
Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity. [PDF]
Since its initial application in mammalian cells, CRISPR-Cas9 has rapidly become a preferred method for genome engineering experiments. The Cas9 nuclease is targeted to genomic DNA using guide RNAs (gRNA), either as the native dual RNA system consisting ...
Megan Basila+2 more
doaj +1 more source