Molecular phenotyping of the pal1 and pal2 mutants of Arabidopsis thaliana reveals far-reaching consequences on phenylpropanoid, amino acid, and carbohydrate metabolism [PDF]
, 2004 The first enzyme of the phenylpropanoid pathway, Phe ammonia-lyase (PAL), is encoded by four genes in Arabidopsis thaliana. Whereas PAL function is well established in various plants, an insight into the functional significance of individual gene family ...
Boerjan, Wout +13 more
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cDNA-AFLP na identificação de genes relacionados a qualidade fisiológica [PDF]
Revista Brasileira de Sementes, 2009 Alguns trabalhos têm evidenciado a existência de genótipos de soja contrastantes para qualidade fisiológica de semente. Tais diferenças existem em virtude da presença de sementes com total ou parcial impermeabilidade do tegumento à água, o que as tornam menos susceptíveis aos danos mecânicos, as adversidades climáticas e a deterioração por umidade.
Mertz, Liliane Marcia +2 more
openaire +3 more sources
Visualization of Differential Gene Expression – Using Fluorescence‐Based cDNA‐AFLP [PDF]
Engineering in Life Sciences, 2004 AbstractcDNA‐AFLP is one of the techniques developed to study differentially expressed genes. This recent technique is advantageous because it does not need prior sequence knowledge and is reliable due to highly stringent PCR conditions.The traditional cDNA‐AFLP method uses radioactively labelled products and is characterised by high sensitivity and ...
S. Gigliotti, J.‐F. Hausman, D. Evers
openaire +1 more source
Components of variance in transcriptomics based on electrophoretic separation of cDNA fragments (cDNA-AFLP). [PDF]
Electrophoresis, 2009 AbstractThe sources of variance and errors in transcriptomics based on the electrophoretic separation of amplified cDNA fragments were investigated using cDNA‐amplified fragment length polymorphism (AFLP). Transcriptome profiles of the plant‐pathogenic fungus Verticillium longisporum were generated by a standard cDNA‐AFLP protocol followed by ...
Weiberg A, Karlovsky P.
europepmc +5 more sources
Transcriptional responses in Honey Bee larvae infected with chalkbrood fungus
BMC Genomics, 2010 Background Diseases and other stress factors working synergistically weaken honey bee health and may play a major role in the losses of bee populations in recent years. Among a large number of bee diseases, chalkbrood has been on the rise.
Murray Keith D +2 more
doaj +1 more source
Transcriptome profile of early responsive genes in susceptible barley during Rhynchosporium secalis infection [PDF]
, 2016 Scald caused by Rhynchosporium secalis, is an economically important disease found worldwide. In order to profile genes and pathways responding to R. seclais infection, leaf transcriptomes before and after fungus inoculation in susceptible barley were ...
Al-Shehadah, E. +5 more
core +1 more source
Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber in response to Phytophthora cinnamomi infection [PDF]
, 2014 cDNA-AFLP methodology was used to gain insight into gene fragments differentially present in the mRNA profiles of Quercus suber roots infected with zoospores of Phytophthora cinnamomi at different post challenge time points.
A Chandra +92 more
core +2 more sources
Robust ordered mRNA differential display: an improved method for global gene expression profiling
BioTechniques, 2011 Global gene expression profiling (GGEP) plays a pivotal role in biological research. We developed an improved GGEP method called “robust ordered mRNA differential display (RoDD)” by combining mRNA differential display (DD) and complementary DNA amplified
Honggao Liu +5 more
doaj +1 more source
Identification of Novel Hypoxia Response Genes in Human Glioma Cell Line A172 [PDF]
Iranian Journal of Basic Medical Sciences, 2013 Objective(s): Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV.
Fatemeh Baghbani +11 more
doaj
(Re)-conciliation of genetics and genomics approaches for cotton fiber quality improvement [PDF]
, 2008 The integration of genomics and plant breeding is driven by the increasing availability of sequence resources and by technological developments. The simultaneous measurement of the expression of thousands of genes is possible, and comparisons between ...
Al-Ghazi, Yves +11 more
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