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Geometrically Encoded Positioning of Introns, Intergenic Segments, and Exons in the Human Genome
Advanced Science, EarlyView.This study introduces a new hypothesis: exons, introns, and intergenic segments are non‐random projections of the functional layers of 3D structure of chromatin packing domains. Evidence is presented that this “geometric code” may encode volumetric structure, reconciling epigenetic patterns, correlates with oncogenic mutations, acting as a potential ...
Luay M. Almassalha +11 more
wiley +1 more source
Protocol for multiplexed transcription factor activity detection using optimized barcoded reporters and an automated computational pipeline. [PDF]
STAR ProtocTrauernicht M +3 more
europepmc +1 more source
Protocol for generating native and unmodified bacterial RNA for nanopore direct RNA sequencing and downstream analysis. [PDF]
STAR ProtocGuo Z, Shao Y, Tan L, Li R.
europepmc +1 more source
Ligase-Catalyzed Transcription and Reverse Transcription of XNA by T3 DNA Ligase. [PDF]
Curr ProtocKhamissi N, Rustico L, Hili R.
europepmc +1 more source
Construction and Screening of a cDNA Library to Isolate Chicken Pituitary Hormone Genes
, 1987 Douglas N. Foster
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Current Protocols in Molecular Biology, 2010
AbstractThe characterization of rare messages in cDNA libraries is complicated by the substantial variations that exist in the abundance levels of different transcripts in cells and tissues. The equalization (normalization) of cDNA is a helpful approach for decreasing the prevalence of abundant transcripts, thereby facilitating the assessment of rare ...
Ekaterina A, Bogdanov +5 more
openaire +2 more sources
AbstractThe characterization of rare messages in cDNA libraries is complicated by the substantial variations that exist in the abundance levels of different transcripts in cells and tissues. The equalization (normalization) of cDNA is a helpful approach for decreasing the prevalence of abundant transcripts, thereby facilitating the assessment of rare ...
Ekaterina A, Bogdanov +5 more
openaire +2 more sources
2013
The construction of full-length cDNA libraries allows researchers to study gene expression and protein interactions and undertake gene discovery. Recent improvements allow the construction of high-quality cDNA libraries, with small amounts of mRNA. In parallel, these improvements allow for the incorporation of adapters into the cDNA, both at the 5' and
Kooiker, Maarten, Xue, Gang Ping
openaire +3 more sources
The construction of full-length cDNA libraries allows researchers to study gene expression and protein interactions and undertake gene discovery. Recent improvements allow the construction of high-quality cDNA libraries, with small amounts of mRNA. In parallel, these improvements allow for the incorporation of adapters into the cDNA, both at the 5' and
Kooiker, Maarten, Xue, Gang Ping
openaire +3 more sources