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2013
The construction of full-length cDNA libraries allows researchers to study gene expression and protein interactions and undertake gene discovery. Recent improvements allow the construction of high-quality cDNA libraries, with small amounts of mRNA. In parallel, these improvements allow for the incorporation of adapters into the cDNA, both at the 5' and
Kooiker, Maarten, Xue, Gang Ping
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The construction of full-length cDNA libraries allows researchers to study gene expression and protein interactions and undertake gene discovery. Recent improvements allow the construction of high-quality cDNA libraries, with small amounts of mRNA. In parallel, these improvements allow for the incorporation of adapters into the cDNA, both at the 5' and
Kooiker, Maarten, Xue, Gang Ping
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Preserving primary cDNA libraries
Analytical Biochemistry, 1987A technique for the long term storage of primary cDNA libraries in a form such that relevant DNA sequences can be readily identified and retrieved is described. cDNA libraries produced using the lambda gt 10 cloning vector were plated out on host bacteria in 0.7% top agarose supplemented with 30% glycerol.
D M, Klinman, D I, Cohen
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Subtractive Hybridization of cDNA Libraries
2003The technique of subtractive hybridization is used to enrich abundant cDNAs that differ between two cell populations. This approach is well suited for systems in which a homogenous cell population is activated in culture with an agent or factor that induces the transcription of genes that are either not present or at low abundance in the unactivated ...
G S, Kelner, R A, Maki
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Production of a Subtracted cDNA Library
Current Protocols in Molecular Biology, 2001AbstractFor some experiments, a complete cDNA library is unnecessary and instead, a subtracted cDNA library is useful. A subtracted cDNA library contains cDNA clones corresponding to mRNAs present in one cell or tissue type and not present in a second type.
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1996
cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR Kris N. Lambert and Valerie M. Williamson Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations David R. Sibson and Michael P.
Ian G. Cowell, Caroline A. Austin
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cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR Kris N. Lambert and Valerie M. Williamson Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations David R. Sibson and Michael P.
Ian G. Cowell, Caroline A. Austin
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1993
Abstract DNA libraries allow the identification, isolation, and characterization of genes expressed in particular cell types or at specific developmental stages. They provide information on primary structures of mRNAs for comparative studies, and can yield molecular markers for use in descriptive or experimental developmental studies.
Akira Kakizuka +3 more
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Abstract DNA libraries allow the identification, isolation, and characterization of genes expressed in particular cell types or at specific developmental stages. They provide information on primary structures of mRNAs for comparative studies, and can yield molecular markers for use in descriptive or experimental developmental studies.
Akira Kakizuka +3 more
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Normalization of cDNA Libraries
2007In a cellular transcriptome, the number of mRNA copies per gene may differ by several orders. In cDNA libraries, performed from mRNA, these proportions are the same. Normalization methods allow us to equalize numbers of gene’s copies in the library. Normalized cDNA libraries are used to discover new genes transcribed at relatively low levels or for ...
Alex S. Shcheglov +3 more
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cDNA libraries from identified neurons
Proceedings of the Royal Society of London. Series B: Biological Sciences, 1996One approach to studying the changes in gene expression which underlie differentiation is to construct cDNA libraries from different tissues or at different stages of development. However, generating representative cDNA libraries from heterogeneous tissues such as the nervous system is often a real problem.
S, Korneev +3 more
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Trends in Biotechnology, 2001
A collaboration between the Riken Genome Exploration Research Group Phase II team and the FANTOM Consortium has produced a collection of full-length complementary DNAs, their sequences and their physical mapping to the mouse genome (Nature, 8 February).
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A collaboration between the Riken Genome Exploration Research Group Phase II team and the FANTOM Consortium has produced a collection of full-length complementary DNAs, their sequences and their physical mapping to the mouse genome (Nature, 8 February).
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Construction of a cDNA library
2001Abstract This chapter primarily describes a protocol for cDNA library construction that has worked routinely in our laboratory. This protocol includes methods for RNA and poly(A+) RNA isolation, complementary DNA (cDNA) synthesis and cDNA cloning.
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