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A Guide for Spatial Omics Technologies: Innovation, Evaluation, and Application
Advanced Science, EarlyView.This review presents a strategy‐centric framework for spatial omics technologies, organizing methods by how spatial information is experimentally encoded. It compares key performance trade‐offs across sequencing‐ and imaging‐based approaches, examines computational and practical limitations, and highlights biomedical applications. The analysis provides
Xiaofeng Wu +5 more
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Transcriptomic data sets examining several stress responses in <i>Zymomonas mobilis</i> strains. [PDF]
Microbiol Resour AnnouncAskenasy I, Myers KS, Kiley PJ.
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Detecting foldback artifacts in long-reads. [PDF]
BMC GenomicsHeinz JM, Meyerson M, Li H.
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A practical guide to targeted single-cell RNA sequencing technologies. [PDF]
Commun BiolMoro G, Brunner E, Basler K.
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The circadian isoform landscape of mouse livers. [PDF]
NAR Genom BioinformZee A +3 more
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Constructing and detecting a cDNA library for mites
Parasitology Research, 2015RNA extraction and construction of complementary DNA (cDNA) library for mites have been quite challenging due to difficulties in acquiring tiny living mites and breaking their hard chitin. The present study is to explore a better method to construct cDNA library for mites that will lay the foundation on transcriptome and molecular pathogenesis research.
Yae Zhao
exaly +3 more sources
Current Protocols in Molecular Biology, 2010
AbstractThe characterization of rare messages in cDNA libraries is complicated by the substantial variations that exist in the abundance levels of different transcripts in cells and tissues. The equalization (normalization) of cDNA is a helpful approach for decreasing the prevalence of abundant transcripts, thereby facilitating the assessment of rare ...
Ekaterina A, Bogdanov +5 more
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AbstractThe characterization of rare messages in cDNA libraries is complicated by the substantial variations that exist in the abundance levels of different transcripts in cells and tissues. The equalization (normalization) of cDNA is a helpful approach for decreasing the prevalence of abundant transcripts, thereby facilitating the assessment of rare ...
Ekaterina A, Bogdanov +5 more
openaire +2 more sources