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Preserving primary cDNA libraries
Analytical Biochemistry, 1987A technique for the long term storage of primary cDNA libraries in a form such that relevant DNA sequences can be readily identified and retrieved is described. cDNA libraries produced using the lambda gt 10 cloning vector were plated out on host bacteria in 0.7% top agarose supplemented with 30% glycerol.
D M, Klinman, D I, Cohen
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cDNA libraries from identified neurons
Proceedings of the Royal Society of London. Series B: Biological Sciences, 1996One approach to studying the changes in gene expression which underlie differentiation is to construct cDNA libraries from different tissues or at different stages of development. However, generating representative cDNA libraries from heterogeneous tissues such as the nervous system is often a real problem.
S, Korneev +3 more
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Subtractive Hybridization of cDNA Libraries
2003The technique of subtractive hybridization is used to enrich abundant cDNAs that differ between two cell populations. This approach is well suited for systems in which a homogenous cell population is activated in culture with an agent or factor that induces the transcription of genes that are either not present or at low abundance in the unactivated ...
G S, Kelner, R A, Maki
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1996
cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR Kris N. Lambert and Valerie M. Williamson Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations David R. Sibson and Michael P.
Ian G. Cowell, Caroline A. Austin
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cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR Kris N. Lambert and Valerie M. Williamson Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations David R. Sibson and Michael P.
Ian G. Cowell, Caroline A. Austin
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1993
Abstract DNA libraries allow the identification, isolation, and characterization of genes expressed in particular cell types or at specific developmental stages. They provide information on primary structures of mRNAs for comparative studies, and can yield molecular markers for use in descriptive or experimental developmental studies.
Akira Kakizuka +3 more
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Abstract DNA libraries allow the identification, isolation, and characterization of genes expressed in particular cell types or at specific developmental stages. They provide information on primary structures of mRNAs for comparative studies, and can yield molecular markers for use in descriptive or experimental developmental studies.
Akira Kakizuka +3 more
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Trends in Biotechnology, 2001
A collaboration between the Riken Genome Exploration Research Group Phase II team and the FANTOM Consortium has produced a collection of full-length complementary DNAs, their sequences and their physical mapping to the mouse genome (Nature, 8 February).
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A collaboration between the Riken Genome Exploration Research Group Phase II team and the FANTOM Consortium has produced a collection of full-length complementary DNAs, their sequences and their physical mapping to the mouse genome (Nature, 8 February).
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cDNA library generation from ribonucleoprotein particles
Nature Protocols, 2011Most, if not all, known noncoding RNAs (ncRNAs) are associated with RNA binding proteins, thus forming ribonucleoprotein particles or RNPs. Here we describe a protocol for the generation of a specialized cDNA library from RNPs, thereby increasing the proportion of functional ncRNA species in the library.
Mathieu, Rederstorff +1 more
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Functional cDNA libraries from Drosophila embryos
Journal of Molecular Biology, 1988We have modified current methods to create a very efficient technique for cloning cDNAs in a defined orientation, into plasmid vectors bearing phage SP6 and T7 polymerase promoters. First strand synthesis is primed at the poly(A) tail with a 26-mer synthetic oligonucleotide linker/primer, the RNA is hydrolyzed and the cDNA is tailed with 10 to 15 dG ...
N H, Brown, F C, Kafatos
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Normalization of cDNA Libraries
2007In a cellular transcriptome, the number of mRNA copies per gene may differ by several orders. In cDNA libraries, performed from mRNA, these proportions are the same. Normalization methods allow us to equalize numbers of gene’s copies in the library. Normalized cDNA libraries are used to discover new genes transcribed at relatively low levels or for ...
Alex S. Shcheglov +3 more
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1991
Abstract Construction of a PCR-directed cDNA library from total RNA may provide the only methodological approach to analyse cell-specific gene expression where the amount of biological tissue is severely restricted. This approach is particularly applicable where a specific stimulus results in the particularly or differentiation of a ...
Sarah Jane Jane, Michael J McPherson
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Abstract Construction of a PCR-directed cDNA library from total RNA may provide the only methodological approach to analyse cell-specific gene expression where the amount of biological tissue is severely restricted. This approach is particularly applicable where a specific stimulus results in the particularly or differentiation of a ...
Sarah Jane Jane, Michael J McPherson
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