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Antibody-Screening cDNA Libraries
1985Molecular cloning and ancillary techniques offer an attractive alternative in studying many biological phenomena. Successful molecular cloning of genes encoding proteins for which amino acid sequence data are not available can be achieved by antibody (Ab) screening.
Frank A. Simmen +6 more
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1999
cDNA libraries are collections of recombinant DNA sequences, derived from and representing entire mRNA populations present at a given stage of development or differentiation in certain tissues or cell types. cDNA libraries can be self-made and tailored according to individual experimental needs.
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cDNA libraries are collections of recombinant DNA sequences, derived from and representing entire mRNA populations present at a given stage of development or differentiation in certain tissues or cell types. cDNA libraries can be self-made and tailored according to individual experimental needs.
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Generating EST Libraries: Trans-Spliced cDNAs
2009Eukaryotes using trans-splicing for transcript processing incorporate a taxon-specific sequence tag (the spliced leader, SL) to a proportion (either all or a fraction) of their mRNAs. This feature may be exploited for the preparation of full-length-enriched cDNA libraries from these organisms (a diverse group including euglenozoa and dinoflagellates ...
Cecilia, Fernández, Rick M, Maizels
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Production of a Subtracted cDNA Library
Current Protocols in Molecular Biology, 2001AbstractFor some experiments, a complete cDNA library is unnecessary and instead, a subtracted cDNA library is useful. A subtracted cDNA library contains cDNA clones corresponding to mRNAs present in one cell or tissue type and not present in a second type.
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cDNA Library Construction from Single Cells
Current Protocols in Neuroscience, 2002AbstractcDNA Library Construction from Single Cells (Phillip R. Kramer, National Institute of Neuronal Disorders and Stroke, NIH, Bethesda, Maryland). Analysis of gene expression or comparing gene expression patterns between cells and/or tissues facilitates the identification of molecules activated by a cellular program or by a particular physiological
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Current Protocols in Molecular Biology, 1988
AbstractThis unit provides a basic description of issues that require careful consideration before undertaking construction of cDNA libraries. Topics include: generating a double‐stranded DNA copy of the mRNA, the quality of the mRNA, the relative abundance of the clone of interest, the choice of screening method, the size of the library that will be ...
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AbstractThis unit provides a basic description of issues that require careful consideration before undertaking construction of cDNA libraries. Topics include: generating a double‐stranded DNA copy of the mRNA, the quality of the mRNA, the relative abundance of the clone of interest, the choice of screening method, the size of the library that will be ...
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Defect activity in metal halide perovskites with wide and narrow bandgap
Nature Reviews Materials, 2021Yang Zhou +2 more
exaly
A single-atom library for guided monometallic and concentration-complex multimetallic designs
Nature Materials, 2022Lili Han, Hao Cheng, Pengfei Ou
exaly