Results 11 to 20 of about 3,190 (165)

Optimisation and Scale-Up of the Heterologous Production of the Antifungal Protein PeAfpA From Penicillium expansum in Stirred-Tank Bioreactors. [PDF]

Microb Biotechnol
This work demonstrates the feasibility of scaling up laboratory processes to pilot scale production of antifungal proteins for diverse applications and exemplifies the possibilities that recyclable CRISPR/Cas9 methodology offers for fungal biotechnology.
de Ovalle S, Garrigues S, Gimeno-Alcañiz JV, Mercader JV, Abad-Fuentes A, Marcos JF, Manzanares P.   +6 more
europepmc   +2 more sources

Development of antigen-capture ELISA using monoclonal antibodies for the detection of brucellae in milk

German Journal of Veterinary Research, 2023
In this study, a Brucella antigen-capture ELISA (Ag-cELISA) prototype was developed. To study the validity of the developed Ag-cELISA, milk samples collected from Brucella-positive goats (n=120) and cattle (n= 64), as well as from unknown Brucella-status
sevil gürbilek, oktay keskin, osman tel
doaj   +1 more source

Competitive ELISA test system for the detection of antibodies to the rabies virus in animals (Rhabdoviridae: Lyssavirus)

Вопросы вирусологии, 2022
Introduction. The main approach to the rabies prevention is the vaccination of domestic and wild carnivores. For the routine evaluation the anti-rabies vaccination effectiveness, World Organization for Animal Health (OIE) recommends various enzyme-linked
Varvara A. Lobanova, Kristina N. Tsarkova, Olesya A. Bogomolova, Irina N. Matveeva, Valentina I. Klyukina   +4 more
doaj   +1 more source

Development and Application of a Nanobody-Based Competitive ELISA for Detecting Antibodies against Hepatitis E Virus from Humans and Domestic Animals

Microbiology Spectrum, 2023
Hepatitis E virus (HEV) is a zoonotic pathogen that is widespread worldwide. At present, most enzyme-linked immunosorbent assay (ELISA) kits only detect antibodies against human HEV.
Yiyang Chen, Meimei Zhang, Tianxiang Chen, Jiaxi Wang, Qin Zhao, En-Min Zhou, Baoyuan Liu   +6 more
doaj   +1 more source

Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of Burkholderia mallei, a Tier 1 select agent.

PLoS Neglected Tropical Diseases, 2021
Glanders is a highly contagious and potentially serious disease caused by Burkholderia mallei, a Tier 1 select agent. In this study, we raised a monoclonal antibody (mAb) against the lipopolysaccharide (LPS) of B.
Ulrich Wernery, Elaine Chan, Rekha Raghavan, Jade L L Teng, Ginu Syriac, Sing-Yung Siu, Marina Joseph, Man-Lung Yeung, Lilong Jia, Jian-Piao Cai, Tsz-Ho Chiu, Susanna K P Lau, Patrick C Y Woo   +12 more
doaj   +1 more source

Development of a novel competitive ELISA based on nanobody-horseradish peroxidase fusion protein for rapid detection of antibodies against avian hepatitis E virus

Poultry Science, 2023
: Avian hepatitis E virus (avian HEV) increases poultry mortality and decreases egg production, leading to huge economic losses worldwide. However, there is no effective serological test for avian HEV.
Tianxiang Chen, Baoyuan Liu, Yiyang Chen, Xueting Wang, Meimei Zhang, Xukun Dang, Qin Zhao, En-Min Zhou   +7 more
doaj   +1 more source

Development and characterisation of a fully recombinant competition ELISA for the diagnosis of foot-and-mouth disease virus infection. [PDF]

Aust Vet J
Infection of livestock by foot‐and‐mouth disease (FMD) virus (FMDV) has serious economic implications due to productivity losses and trade restrictions. In countries that are free from foot and mouth disease, the World Organisation for Animal Health (WOAH) provides two proof of freedom from disease status categories; namely, FMD‐free without using ...
Ye S, Balasubramanian NS, Dolezal O, Foord A, Beddome G, Michalski WP, Shiell BJ, Peck GR.   +7 more
europepmc   +2 more sources

Performance Analysis of Anaplasma Antibody Competitive ELISA Using the ROC Curve for Screening of Anaplasmosis in Camel Populations in Egypt

Pathogens, 2020
Anaplasmosis is a tick-born and potential zoonotic disease caused by Anaplasma (A.) phagocytophilum, A. ovis, A. platys and A. capra. Anaplasma marginale affecting bovines and camels causing significant economic losses.
Omid Parvizi, Hosny El-Adawy, Uwe Roesler, Heinrich Neubauer, Katja Mertens-Scholz   +4 more
doaj   +1 more source

Prevalence of Peste Des Petits Ruminants Virus (PPRV) in Mardan, Hangu and Kohat District of Pakistan; Comparative Analysis of PPRV Suspected serum samples using Competitive ELISA (cELISA) and Agar Gel Immunodiffusion (AGID) [PDF]

Veterinary World, 2009
Peste des petits Ruminants (PPR) is an acute, febrile, highly contagious and economically important viral disease of small ruminants. However PPR is more prevalent in sheep and goat.
Misbah Aslam, Muhammad Abubakar, Rehana Anjum, Shamim Saleha, and Qurban Ali   +4 more
doaj   +1 more source

Seroprevalence of Contagious Caprine Pleuropneumonia in Selected Districts of Borana Zone, Oromia Region, Southern Ethiopia. [PDF]

Vet Med Sci
A cross‐sectional study, conducted on contagious caprine pleuropneumonia (CCPP) in the goat population, revealed a seroprevalence of 15.85% (95% CI: 12.06%–20.54%). Undertaking a broader research involving a larger‐scale study and reservoir species, including sheep, is recommended to gain deeper practical insights into the disease.
Desa G, Benti T, Zewde D.
europepmc   +2 more sources