Results 21 to 30 of about 2,073 (157)

Clonagem, expressão, caracterização molecular da proteína de superfície MSP5 da amostra PR1 de Anaplasma marginale e sua aplicação em um teste de ELISA por competição Cloning, expression, molecular characterization of the MSP5 protein from PR1 strain of Anaplasma marginale and its application in a competitive enzyme-linked immunosorbent test

Revista Brasileira de Parasitologia Veterinária, 2009
No presente trabalho, um teste imunoenzimático por competição (cELISA) foi padronizado com a proteína recombinante de superfície rMSP5, clonada a partir do gene msp5 do isolado PR1 de A. marginale. O sequenciamento mostrou que o gene que codifica a rMSP5/
Elizabete Regina M. Marana, Flora S. Kano, Josy C. Vicentini, Rafael S. Spurio, Marcela Ribeiro, Adriana Letícia M. Coelho, Marilda C. Vidotto, Odilon Vidotto   +7 more
doaj   +1 more source

Seroprevalence of contagious caprine pleuropneumonia in small ruminants in Karnataka, India

Indian Journal of Animal Sciences, 2020
The objective of this study was to investigate the prevalence of Contagious caprine pleuropneumonia (CCPP) in small ruminants in Hassan district, Karnataka, India and to evaluate the accuracy of Slide Agglutination Test (SAT) over competitive ELISA ...
K M CHANDRASHEKAR, G S NAVEEN KUMAR, N CHANDRASHEKHARA, J PUNYA, L RANGANATH   +4 more
doaj   +1 more source

Prevalence of Anaplasma ovis infection in Angora goats of Duhok province, Kurdistan region-Iraq [PDF]

Iraqi Journal of Veterinary Sciences, 2017
Acute Anaplasma ovis infections can cause severe clinical symptoms and might lead to significant economic losses in small ruminant flocks. However, little information has been provided related the prevalence of anaplasmosis in Angora goats. The study was
I.A. Naqid
doaj   +1 more source

A Novel Competitive ELISA for Specifically Measuring and Differentiating Immune Responses to Classical Swine Fever C-Strain Vaccine in Pigs

Viruses, 2022
Classical swine fever can be controlled effectively by vaccination with C-strain vaccine. In this study, we developed a novel competitive enzyme-linked immunosorbent assay (cELISA) based on a C-strain Erns specific monoclonal antibody (mAb 1504), aiming ...
Lihua Wang, Shijiang Mi, Rachel Madera, Yuzhen Li, Wenjie Gong, Changchun Tu, Jishu Shi   +6 more
doaj   +1 more source

Prevalence of Liver Fluke (Fasciola hepatica) in Wild Red Deer (Cervus elaphus): Coproantigen ELISA Is a Practicable Alternative to Faecal Egg Counting for Surveillance in Remote Populations.

PLoS ONE, 2016
Red deer (Cervus elaphus) are hosts of liver fluke (Fasciola hepatica); yet, prevalence is rarely quantified in wild populations. Testing fresh samples from remote regions by faecal examination (FE) can be logistically challenging; hence, we appraise ...
Andrew S French, Ruth N Zadoks, Philip J Skuce, Gillian Mitchell, Danielle K Gordon-Gibbs, Alexandra Craine, David Shaw, Stuart W Gibb, Mark A Taggart   +8 more
doaj   +1 more source

An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA [PDF]

BMC Veterinary Research, 2014
AbstractBackgroundFew serological tests are available for detecting antibodies againstMycoplasma capricolumsubsp.capripneumoniae, the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, the test prescribed for international trade purposes, uses a crude antigen that cross-reacts with all the other mycoplasma species ...
Peyraud, Armelle, Poumarat, François, Tardy, Florence, Manso-Silván, Lucía, Hamroev, Karomatullo, Tilloev, Tillo, Amirbekov, Mullojon, Tounkara, Karim, Bodjo, Charles, Wesonga, Hezron, Nkando, Isabel G., Jenberie, Shiferaw, Yami, Martha, Cardinale, Eric, Meenowa, Deodass, Jaumally, Mahmad R., Yaqub, Tahir, Shabbir, Muhammad Z., Mukhtar, Nadia, Halimi, Mohibullah, Ziay, Ghulam M., Schauwers, Willy, Noori, Hafizullah, Rajabi, Ali M., Ostrowski, Stéphane, Thiaucourt, François   +25 more
openaire   +6 more sources

Serological survey of bluetongue virus in sheep from Minas Gerais [PDF]

Pesquisa Veterinária Brasileira, 2020
: Bluetongue is an infectious, non-contagious disease that affects domestic and wild ruminants, caused by a virus from the Orbivirus genus, Reoviridae family, transmitted by arthropod vectors of the Culicoides genus.
Daniel A. Biihrer, Adriana S. Albuquerque, Adriana H.C.N. Romaldini, Edviges M. Pituco, Ana Carolina D. Matos, Zelia I.P. Lobato, Mary S. Varaschin, Djeison L. Raymundo   +7 more
doaj   +2 more sources

Agreement between commercial assays for haptoglobin and serum amyloid A in goats

Acta Veterinaria Scandinavica, 2017
Haptoglobin (Hp) and serum amyloid A (SAA) are considered as the major acute phase proteins (APPs) in goats. These APPs have been investigated in several studies during the last decade.
Michał Czopowicz, Olga Szaluś-Jordanow, Marcin Mickiewicz, Agata Moroz, Lucjan Witkowski, Iwona Markowska-Daniel, Daria Reczyńska, Emilia Bagnicka, Jarosław Kaba   +8 more
doaj   +1 more source

Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies

AMB Express, 2021
Virus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which are similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed ...
Manyuan Bai, Rui Wang, Shiqi Sun, Yun Zhang, Hu Dong, Huichen Guo   +5 more
doaj   +1 more source

Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody

Frontiers in Veterinary Science, 2023
IntroductionNipah virus (NiV) and Hendra virus (HeV), of the genus Henipavirus, family Paramyxoviridae, are classified as Risk Group 4 (RG4) pathogens that cause respiratory disease in pigs and acute/febrile encephalitis in humans with high mortality ...
Wenjun Zhu, Bradley Pickering, Bradley Pickering, Bradley Pickering, Greg Smith, Mathieu Pinette, Thang Truong, Shawn Babiuk, Shawn Babiuk, Darwyn Kobasa, Darwyn Kobasa, Logan Banadyga, Logan Banadyga, Ming Yang   +13 more
doaj   +1 more source