Results 31 to 40 of about 2,073 (157)

INDIRECT AND COMPETITIVE ELISA AS A TOOL FOR DIAGNOSIS OF BRUCELLOSIS IN VACCINATED AND INFECTED CATTLE [PDF]

Assiut Veterinary Medical Journal, 2011
The results of an indirect ELISA (iELISA) and a competitive ELISA (cELISA) for detection of antibody to Brucella melitensis in cows were compared with those of conventional serological tests.
M.M. BASSIONY, I.G.A. IBRAHIM, EMAN S. RAMADAN   +2 more
doaj   +1 more source

Applicability of the Filter Paper Technique for Detection of Antifilarial IgG(4) Antibodies Using the Bm14 Filariasis CELISA.

Journal of parasitology research, 2010
Demonstration of successful elimination of lymphatic filariasis (LF) in endemic countries requires sensitive diagnostics for accurate definitions of endpoints and future surveillance. There has been interest in complementing available diagnostics with antibody serology testing in children, since negative serology would correspond with cessation of LF ...
Joseph, HM, Melrose, W
openaire   +5 more sources

Epidemiological and Comparative Diagnostic Study of Anaplasma Spp. Infection in Goats from North-Eastern Algeria

Folia Veterinaria, 2020
Anaplasmosis is an infectious disease affecting goats and other ruminants. Our goal was to estimate the prevalence of Anaplasma spp. infections, and to identify different tick species in goa ts along with their associated risk factors.
Reghaissia N., Dahmane A., Boularias G., Ghalmi F., Azzag N.   +4 more
doaj   +1 more source

Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Journal of Nanobiotechnology, 2019
Background Sensitive and specific antibodies can be used as essential probes to develop competitive enzyme-linked immunosorbent assay (cELISA).
Yamin Sheng, Kun Wang, Qizhong Lu, Pinpin Ji, Baoyuan Liu, Jiahong Zhu, Qingyuan Liu, Yani Sun, Jingfei Zhang, En-Min Zhou, Qin Zhao   +10 more
doaj   +1 more source

Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania.

PLoS Neglected Tropical Diseases, 2021
BackgroundBrucellosis is a neglected zoonosis endemic in many countries, including regions of sub-Saharan Africa. Evaluated diagnostic tools for the detection of exposure to Brucella spp.
Rebecca F Bodenham, Stella Mazeri, Sarah Cleaveland, John A Crump, Folorunso O Fasina, William A de Glanville, Daniel T Haydon, Rudovick R Kazwala, Tito J Kibona, Venance P Maro, Michael J Maze, Blandina T Mmbaga, Niwael J Mtui-Malamsha, Gabriel M Shirima, Emanuel S Swai, Kate M Thomas, Barend M deC Bronsvoort, Jo E B Halliday   +17 more
doaj   +1 more source

A neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post–vaccination monitoring

BMC Veterinary Research, 2020
Background Virus neutralization test (VNT) is widely used for serological survey of classical swine fever (CSF) and efficacy evaluation of CSF vaccines. However, VNT is a time consuming procedure that requires cell culture and live virus manipulation.
Lihua Wang, Shijiang Mi, Rachel Madera, Llilianne Ganges, Manuel V. Borca, Jingqiang Ren, Chase Cunningham, Ada G. Cino-Ozuna, Hongwei Li, Changchun Tu, Wenjie Gong, Jishu Shi   +11 more
doaj   +1 more source

Optimization of Cellular ELISA for Assay of Surface Antigens on Human Synoviocytes

BioTechniques, 1997
The cellular enzyme-linked immunosorbent assay (CELISA) permits assay of cell surface antigens on intact fixed cells. Using a monolayer of cells as the solid phase, the CELISA offers an inexpensive alternative to flow cytometry.
Donald D. Smith, Christopher B. Cohick, Herbert B. Lindsley   +2 more
doaj   +1 more source

A nanobody‐horseradish peroxidase fusion protein‐based competitive ELISA for rapid detection of antibodies against porcine circovirus type 2

Journal of Nanobiotechnology, 2021
Background The widespread popularity of porcine circovirus type 2(PCV2) has seriously affected the healthy development of the pig industry and caused huge economic losses worldwide.
Yang Mu, Cunyu Jia, Xu Zheng, Haipeng Zhu, Xin Zhang, Haoran Xu, Baoyuan Liu, Qin Zhao, En-Min Zhou   +8 more
doaj   +1 more source

Comparison of a Flow Assay for Brucellosis Antibodies with the Reference cELISA Test in West African Bos indicus

PLoS ONE, 2009
Brucellosis is considered by the Food and Agricultural Organisation and the World Health Organisation as one of the most widespread zoonoses in the world. It is a major veterinary public health challenge as animals are almost exclusively the source of infection for people.
Bronsvoort, B. M., Koterwas, B., Land, F., Handel, Ian, Tucker, J., Morgan, K. L., Tanya, V. N., Abdoel, T. H., Smits, H. L.   +8 more
openaire   +5 more sources

Seroprevalence of Antibodies Against Anaplasma spp. in Algerian Sheep and Associated Risk Factors [PDF]

Archives of Razi Institute
This study aimed to determine the seroprevalence and identify the main risk factors associated with exposure to Anaplasma spp. in sheep from two Algerian regions, Médéa and Bordj Bou Arréridj. Between March and November 2021, a total of 361 blood samples
Mira Rima Haddoum, Omar Salhi, Mohamed Rahal, Abdelaziz Lounas, Safia Zenia, Nassim Ouchene, Nadjet Amina Khelifi Touhami, Farida Ghalmi, Naouelle Azzag   +8 more
doaj   +1 more source