Cell‐Free DNA‐Based Theranostics for Inflammatory Disorders [PDF]
Advanced ScienceInflammatory disorders are characterized by immune‐mediated inflammatory cascades that can affect multiple organs. Cell‐free DNA (cfDNA) is not only a biomarker for various inflammatory diseases, but also a driver in innate immune responses, offering ...
Jiatong Li +7 more
doaj +2 more sources
Changes in the nuclear distribution of DNA polymerase alpha and PCNA/cyclin during the progress of the cell cycle, in a cell-free extract of Xenopus eggs [PDF]
, 1989 The nuclear distribution of DNA polymerase alpha and PCNA/cyclin in embryonic nuclei has been investigated, in a cell-free extract of Xenopus eggs that recapitulates a basic cell-cycle in vitro, by indirect immunofluorescence microscopy.
Kill, IR, Hutchison, C
core +6 more sources
Phenotypes from cell-free DNA [PDF]
Open Biology, 2020 Cell-free DNA (cfDNA) has the potential to enable non-invasive detection of disease states and progression. Beyond its sequence, cfDNA also represents the nucleosomal landscape of cell(s)-of-origin and captures the dynamics of the epigenome.
Alexis Zukowski +2 more
doaj +1 more source
Identifying a role for Ciz1 in the DNA damage response [PDF]
, 2011 In proliferating cells, cell cycle arrest and DNA repair are induced upon detection of DNA damage. The DNA damage response (DDR) to double strand DNA breaks is mediated by phosphatidylinositol 3-kinase-like protein kinases (PIKKs), which propagate DDR ...
Roper, Katherine
core +6 more sources
Cell-free fetal DNA and cell-free total DNA levels in spontaneous abortion with fetal chromosomal aneuploidy. [PDF]
PLoS ONE, 2013 Cell-free fetal DNA and cell-free total DNA in maternal circulation have been proposed as potential markers for noninvasive monitoring of the placental condition during the pregnancy. However, the correlation of and change in cell-free fetal DNA and cell-
Ji Hyae Lim +7 more
doaj +1 more source
Isolation and Quantification of Plasma Cell-Free DNA Using Different Manual and Automated Methods
Diagnostics, 2022 Plasma cell-free DNA (cfDNA) originates from various tissues and cell types and can enable minimally invasive diagnosis, treatment and monitoring of cancer and other diseases.
Eleni Polatoglou +4 more
doaj +1 more source
Ciz1 cooperates with cyclin-A-CDK2 to activate mammalian DNA replication in vitro [PDF]
, 2010 Initiation of mammalian DNA replication can be reconstituted from isolated G1-phase nuclei and cell extracts, supplemented with cyclin-dependent protein kinases (CDKs).
Sercombe, Heather E +3 more
core +4 more sources
Plasma contains ultrashort single-stranded DNA in addition to nucleosomal cell-free DNA
iScience, 2022 Summary: Plasma cell-free DNA is being widely explored as a biomarker for clinical screening. Currently, methods are optimized for the extraction and detection of double-stranded mononucleosomal cell-free DNA of ∼160bp in length. We introduce uscfDNA-seq,
Jordan Cheng +13 more
doaj +1 more source
Plasma Cell-Free DNA After Embolization
Journal of Vascular Anomalies, 2023 Objectives:. To identify genetic variants in venous malformations for noninvasive diagnosis using peripheral plasma Cell-free DNA (cfDNA) after ethanol embolization. Method:.
Yi Sun +10 more
doaj +1 more source
Biomarker Research, 2023 Background Recent advances in circulating cell-free DNA (cfDNA) analysis from biofluids have opened new avenues for liquid biopsy (LB). However, current cfDNA LB assays are limited by the availability of existing information on established genotypes ...
Neeti Swarup +14 more
doaj +1 more source