Results 1 to 10 of about 1,330,071 (148)

Cell-based assays to identify novel retinoprotective agents. [PDF]

MethodsX, 2020
Degeneration of the retina can lead ultimately to devastating irreversible vision loss, such as in inherited retinitis pigmentosa and age-related macular degeneration. Currently there is no cure to prevent retinal degeneration. Quantitative cell-based assays can be used to test potential drugs that prevent the death of retinal cells.
Bullock J, Pagan-Mercado G, Becerra SP.
europepmc   +4 more sources

Polystyrene Topography Sticker Array for Cell-Based Assays. [PDF]

Recent Prog Mater, 2020
Cells can respond to different topographical cues in their natural microenvironment. Hence, scientists have employed microfabrication techniques and materials to generate culture substrates containing topographies for cell-based assays. However, one of the limitations of custom topographical platforms is the lack of adoption by the broad research ...
Rosado-Galindo H, Domenech M.
europepmc   +3 more sources

Cordycepin attenuates migration and invasion of HSC-4 oral squamous carcinoma cells through autophagy-dependent FAK/Akt and MMP2/MMP9 suppression

Journal of Dental Sciences, 2022
Background/purpose: Cordycepin has been proposed anti-cancer effects, however, it is unclear whether and how cordycepin affects oral squamous carcinoma cell (OSCC) migration and invasion.
Thunwa Binlateh, Nawapol Uppatcha, Jutamast Thepchai, Yanaphat Pleungtuk, Parinya Noisa, Pilaiwanwadee Hutamekalin, Paiboon Jitprasertwong   +6 more
doaj   +1 more source

Protocol for altering connexin hemichannel function in primary chicken lens fiber cells using high-titer retroviral RCAS(A) infection

STAR Protocols, 2023
Summary: Connexins (Cxs) play a crucial role in maintaining lens transparency. Here, we present a protocol for altering Cx hemichannel (HC) function in primary chicken lens fiber cells using high-titer retroviral replication competent avian sarcoma ...
Yu Du, Francisca M. Acosta, Jianping Zhang, Yuxin Tong, Yumeng Quan, Sumin Gu, Jean X. Jiang   +6 more
doaj   +1 more source

Ex vivo assay to evaluate the efficacy of drugs targeting sphingolipids in preventing SARS-CoV-2 infection of nasal epithelial cells

STAR Protocols, 2021
Summary: This protocol enables the testing of drugs against infection of epithelial cells with SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2), using pseudo-typed replication deficient vesicular stomatitis virus particles (pp-VSV) presenting
Katrin Anne Becker, Alexander Carpinteiro, Markus Hoffmann, Stefan Pöhlmann, Johannes Kornhuber, Erich Gulbins   +5 more
doaj   +1 more source

Protocol for assessing ex vivo lipolysis of murine adipose tissue

STAR Protocols, 2022
Summary: Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic ...
Debasish Roy, Julia M. Myers, Andrea Tedeschi   +2 more
doaj   +1 more source

Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assay

STAR Protocols, 2022
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein mediates membrane fusion between the virus and the target cells, triggering viral entry into the latter.
Hesham Nasser, Ryo Shimizu, Jumpei Ito, Akatsuki Saito, Kei Sato, Terumasa Ikeda, Keita Matsuno, Naganori Nao, Hirofumi Sawa, Mai Kishimoto, Shinya Tanaka, Masumi Tsuda, Lei Wang, Yoshikata Oda, Marie Kato, Zannatul Ferdous, Hiromi Mouri, Kenji Shishido, Takasuke Fukuhara, Tomokazu Tamura, Rigel Suzuki, Hayato Ito, Daichi Yamasoba, Izumi Kimura, Naoko Misawa, Keiya Uriu, Yusuke Kosugi, Shigeru Fujita, Mai Suganami, Mika Chiba, Ryo Yoshimura, So Nakagawa, Jiaqi Wu, Akifumi Takaori-Kondo, Kotaro Shirakawa, Kayoko Nagata, Yasuhiro Kazuma, Ryosuke Nomura, Yoshihito Horisawa, Yusuke Tashiro, Yugo Kawai, Takashi Irie, Ryoko Kawabata, MST Monira Begum, Otowa Takahashi, Kimiko Ichihara, Takamasa Ueno, Chihiro Motozono, Mako Toyoda, Yuri L. Tanaka, Erika P. Butlertanaka, Maya Shofa, Kazuo Takayama, Rina Hashimoto, Sayaka Deguchi, Takao Hashiguchi, Tateki Suzuki, Kanako Kimura, Jiei Sasaki, Yukari Nakajima, Kaori Tabata   +60 more
doaj   +1 more source

Protocol for the determination of intracellular phase separation thresholds

STAR Protocols, 2021
Summary: To date, phase separation studies have largely been limited to in vitro assays using non-native conditions and aggregation-prone recombinant proteins that are often difficult to purify.
Anna K. Lee, Patrick Ryan Potts
doaj   +1 more source

Analysis of mannosidase I activity in interphase and mitotic cells by lectin staining and endoglycosidase H treatment

STAR Protocols, 2023
Summary: N-Glycosylation is a common protein modification catalyzed by a series of glycosylation enzymes in the endoplasmic reticulum and Golgi apparatus.
Jie Li, Jianchao Zhang, Yanzhuang Wang
doaj   +1 more source

Detection of DNA damage by alkaline comet assay in mouse colonic mucosa

STAR Protocols, 2021
Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet, Maria M. Haykal, Saleha Hashim, Teresa Frisan, Océane C.B. Martin   +4 more
doaj   +1 more source