Results 171 to 180 of about 4,735,099 (379)

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

open access: yesFEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li   +2 more
wiley   +1 more source

Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer

open access: yesSTAR Protocols
Summary: Bioluminescence resonance energy transfer (BRET) allows to quantitate protein interactions in intact cells. Here, we present a protocol for measuring BRET due to transient interactions of oncogenic K-RasG12V in plasma membrane nanoclusters of ...
Carla Jane Duval   +3 more
doaj   +1 more source

Small Molecules Targeting 3C Protease Inhibit FMDV Replication and Exhibit Virucidal Effect in Cell-Based Assays. [PDF]

open access: yesViruses, 2023
Theerawatanasirikul S   +5 more
europepmc   +1 more source

An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests [PDF]

open access: yes, 2018
This paper proposes a new yet efficient method allowing a significant improvement in the on-line analysis of biological cell growing and evolution.
Daza Navarro, María Paula   +9 more
core  

Cell-Based Assays [PDF]

open access: yesSLAS Discovery, 1997
openaire   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

open access: yesFEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan   +5 more
wiley   +1 more source

Protocol for identifying OXCT1-mediated LACTB succinylation sites in vitro

open access: yesSTAR Protocols
Summary: OXCT1 acts as a succinyltransferase to promote serine beta-lactamase-like protein (LACTB) K284 succinylation. Here, we present a protocol for detecting OXCT1-mediated LACTB succinylation levels and sites.
Wenhao Ma, Yuchen Sun, Huafeng Zhang
doaj   +1 more source

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