Results 261 to 270 of about 4,481,323 (329)

Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer

STAR Protocols
Summary: Bioluminescence resonance energy transfer (BRET) allows to quantitate protein interactions in intact cells. Here, we present a protocol for measuring BRET due to transient interactions of oncogenic K-RasG12V in plasma membrane nanoclusters of ...
Carla Jane Duval, Candy Laura Steffen, Karolina Pavic, Daniel Kwaku Abankwa   +3 more
doaj  

A Sensitive Flow Cytometry-based Nucleotide Excision Repair Assay Unexpectedly Reveals That Mitogen-activated Protein Kinase Signaling Does Not Regulate the Removal of UV-induced DNA Damage in Human Cells [PDF]

, 2007
Raphaël Rouget, Yannick Auclair, Martin Loignon, El Bachir Affar, Elliot Drobetsky   +4 more
openalex   +1 more source

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

Molecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka, Tatsuo Nakagawa, Yusuke Ono, Yoshio Kamura, Takeshi Ishida, Hidemasa Kawabata, Kenji Takahashi, Hiroki Sato, Andrew S. Liss, Yusuke Mizukami, Takahide Yokoi   +10 more
wiley   +1 more source

Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique

STAR Protocols
Summary: Extracellular vesicles (EVs) play a key role in cancer development and cellular homeostasis by transferring the biological cargo to recipient cells. Here, we describe steps for screening EV secretion-related genes by combining a microRNA (miRNA)
Tomofumi Yamamoto, Fumihiko Urabe, Yusuke Yoshioka, Yusuke Yamamoto, Takahiro Ochiya   +4 more
doaj  

A Novel Method for Interpretation of T-Cell Receptor γ Gene Rearrangement Assay by Capillary Gel Electrophoresis Based on Normal Distribution [PDF]

, 2007
Frank C. Kuo, Dimity Hall, Janina A. Longtine   +2 more
openalex   +1 more source

Exploration of heterogeneity and recurrence signatures in hepatocellular carcinoma

Molecular Oncology, EarlyView.
This study leveraged public datasets and integrative bioinformatic analysis to dissect malignant cell heterogeneity between relapsed and primary HCC, focusing on intercellular communication, differentiation status, metabolic activity, and transcriptomic profiles.
Wen‐Jing Wu, Jianchao Wang, Fuqing Chen, Xuefeng Wang, Bin Lan, Ruyi Fu, Hong Wen, Fangfang Chen, Wei Hong, Tian‐Yu Tang, Ying He, Gang Chen, Jianyin Zhou, Hai‐Long Piao, Di Chen, Shu‐Yong Lin   +15 more
wiley   +1 more source

Polystyrene Topography Sticker Array for Cell-Based Assays. [PDF]

Recent Prog Mater, 2020
Rosado-Galindo H, Domenech M.
europepmc   +1 more source

Identification of Novel Human High-Density Lipoprotein Receptor Up-regulators Using a Cell-Based High-Throughput Screening Assay [PDF]

, 2007
Yuan Yang, Zhongbing Zhang, Wei Jiang, Lei Gao, Guiyu Zhao, Zhihui Zheng, Min Wang, Shuyi Si, Bin Hong   +8 more
openalex   +1 more source

Cell-Based Assays for Screening Androgen Receptor Ligands

Seminars in Reproductive Medicine, 2015
Carmela Campana, V. Pezzi, W. Rainey
semanticscholar   +1 more source

Comparing self‐reported race and genetic ancestry for identifying potential differentially methylated sites in endometrial cancer: insights from African ancestry proportions using machine learning models

Molecular Oncology, EarlyView.
Integrating ancestry, differential methylation analysis, and machine learning, we identified robust epigenetic signature genes (ESGs) and Core‐ESGs in Black and White women with endometrial cancer. Core‐ESGs (namely APOBEC1 and PLEKHG5) methylation levels were significantly associated with survival, with tumors from high African ancestry (THA) showing ...
Huma Asif, J. Julie Kim
wiley   +1 more source