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The Problems with the Cells Based Assays
Cell Biology: Research & Therapy, 2012The Problems with the Cells Based Assays An important part of the classical drug discovery paradigm is the cell based assay. Medicinal chemists, after identification of the molecular target such as enzyme or receptor are quite efficient with the design and synthesis of a potential inhibitor or receptor antagonist.
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Cell-based assay for β-secretase activity
Analytical Biochemistry, 2003The cerebral deposition of amyloid beta-peptide (Abeta) is a major factor in the etiology of Alzheimer's disease. beta-Secretase (BACE) initiates the generation of Abeta by cleaving the amyloid precursor protein at the beta-site and is therefore a prime target for therapeutic intervention. Here we report a cell-based method suitable for monitoring BACE
Myungsok, Oh +6 more
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Cell-based assays: fuelling drug discovery
Analytical and Bioanalytical Chemistry, 2010It has been estimated that over a billion dollars in resources can be consumed to obtain clinical approval, and only a few new chemical entities are approved by the US Food and Drug Administration (FDA) each year. Therefore it is of utmost importance to obtain the maximum amount of information about biological activity, toxicological profile ...
MICHELINI, ELISA +4 more
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2017
Tumor necrosis factor alpha (TNFa) not only plays a key role in the human immune system, but also has a cytotoxic effect on tumor cells. Owing to this function, TNFa can become a considerable candidate for chemotherapy but needs to be delivered locally.
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Tumor necrosis factor alpha (TNFa) not only plays a key role in the human immune system, but also has a cytotoxic effect on tumor cells. Owing to this function, TNFa can become a considerable candidate for chemotherapy but needs to be delivered locally.
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Cell-Based Assays for Regulators of Tau Biology
2010Understanding the molecular mechanisms of Alzheimer's disease (AD) is a challenging endeavor, namely, due to the fact that the disease only occurs in the central nervous system of elderly humans. Thus, model systems simply do not accurately portray this cellular landscape. While we cannot ask many mechanistic questions using the human brain as our test
Umesh K, Jinwal, Chad A, Dickey
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Cell-based assays on microfluidic chips
2023In view of miniaturization, different microfluidic devices have been designed and developed to overcome the difficulties of conventional cell-based assays in terms of throughput, single-cell isolation, cost, and flexibility. These platforms are advantageous because of their low reagent consumption, small amount of sample required, as well as facile ...
Hosseini, Motaharesadat +2 more
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Label-Free Cell-Based Functional Assays
Combinatorial Chemistry & High Throughput Screening, 2008Label-free technologies based on electrical impedance or refractive index are new tools for measuring a cell-based functional response. Although the technologies are relatively new to high throughput screening cell-based applications, they are rapidly generating interest in that they are able to measure a phenotypic response using cells natively ...
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A cell-based assay for screening lipoxygenase inhibitors
Prostaglandins & Other Lipid Mediators, 2009Lipoxygenases (LOX) form a family of lipid peroxidizing enzymes within the plant and animal kingdoms. In humans, six functional lipoxygenase isoforms have been identified. 5-LOX, "platelet-type" 12-LOX (p12-LOX) and 15-LOX type 1 (15-LOX1), originally identified in leukocytes, platelets, and reticulocytes, respectively, generate lipid mediators ...
Dileep G, Nair, Colin D, Funk
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High-throughput cell-based assays in yeast
Drug Discovery Today, 2002With the sequencing of many genomes now completed, biologists are faced with the challenge of deciphering the function and association of an immense number of predicted proteins. Comprehension of the networks of proteins and chemicals that comprise the cells and tissues of an organism, and the specific roles of proteins in these networks, will be a ...
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Cell Based Biological Assay Using Microfluidics
2008A microfluidic device has been designed to measure and manipulate microdroplets, in which protein expression is induced in single cells. The device exploits the permeation of water through poly (dimethylsiloxane) (PDMS) in order to keep the concentration of solutes in aqueous picoliter volume microdroplets stored in wells.
Shim +5 more
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