Results 31 to 40 of about 2,906,730 (289)
A novel, resistance-linked ovine PrP variant and its equivalent mouse variant modulate the in vitro cell-free conversion of rPrP to PrPres [PDF]
, 2006 Prion diseases are associated with the conversion of the normal cellular prion protein, PrPc, to the abnormal, disease-associated form, PrPSc. This conversion can be mimicked in vitro by using a cell-free conversion assay. It has recently been shown that
Gill, Andrew C. +4 more
core +1 more source
Phenotypes from cell-free DNA [PDF]
Open Biology, 2020 Cell-free DNA (cfDNA) has the potential to enable non-invasive detection of disease states and progression. Beyond its sequence, cfDNA also represents the nucleosomal landscape of cell(s)-of-origin and captures the dynamics of the epigenome. In this review, we highlight the emergence of cfDNA epigenomic methods that assess disease beyond the scope of ...
Alexis Zukowski +2 more
openaire +3 more sources
A high-throughput screening method for determining the substrate scope of nitrilases [PDF]
, 2014 Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and
Black, Gary +5 more
core +1 more source
The pitfalls and promise of liquid biopsies for diagnosing and treating solid tumors in children : a review [PDF]
, 2020 Cell-free DNA profiling using patient blood is emerging as a non-invasive complementary technique for cancer genomic characterization. Since these liquid biopsies will soon be integrated into clinical trial protocols for pediatric cancer treatment ...
Astrahantseff, Kathy +12 more
core +2 more sources
Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system. [PDF]
, 2018 Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed by the cell.
Joseph, Simpson +4 more
core +2 more sources
Annals of Mechnikov's Institute, 2020 Introduction. Precursor directed biosynthesis is one of the promising approaches to finding new antimicrobial agents and creating next-generation probiotics. L.
O.V Knysh +3 more
doaj
Microscopy Today, 2000 Abstract Regulated secretion occurs when the ceil detects a signal, vesicles containing the secretory product fuse with the plasma membrane of the cell, and the contents are released into the extracellular space. This mechanism is called exocytosis. Many laboratories around the world have studied exocytosis for the past couple of decades,
openaire +1 more source
Scalable Cell-Free Massive MIMO System: Joint Optimal Pilot and Access Point Assignment
IEEE AccessCell-free massive MIMO (CF-mMIMO) is a promising topology for 6G wireless networks which can potentially improve the network capacity. Pilot contamination, network overloading and scalability are the main challenges in the design of CF-mMIMO systems.
Mhammad B. Hmayed, Ali H. Bastami
doaj +1 more source
Models for Cell-Free Synthetic Biology: Make Prototyping Easier, Better, and Faster
Frontiers in Bioengineering and Biotechnology, 2018 Cell-free TX-TL is an increasingly mature and useful platform for prototyping, testing, and engineering biological parts and systems. However, to fully accomplish the promises of synthetic biology, mathematical models are required to facilitate the ...
Mathilde Koch +5 more
doaj +1 more source
Cell-free prediction of protein expression costs for growing cells [PDF]
, 2017 Translating heterologous proteins places significant burden on host cells, consuming expression resources leading to slower cell growth and productivity.
Borkowski, O +5 more
core +5 more sources