Results 11 to 20 of about 10,893,804 (303)

Cell-free riboswitches

open access: yesRSC Chemical Biology, 2021
Synthetic riboswitches can be used as chemical gene switches in cell-free protein synthesis systems. We provide a current perspective on the state of cell-free riboswitch technologies and their future directions.
Takeshi Tabuchi, Yohei Yokobayashi
openaire   +3 more sources

Changes in the nuclear distribution of DNA polymerase alpha and PCNA/cyclin during the progress of the cell cycle, in a cell-free extract of Xenopus eggs [PDF]

open access: yes, 1989
The nuclear distribution of DNA polymerase alpha and PCNA/cyclin in embryonic nuclei has been investigated, in a cell-free extract of Xenopus eggs that recapitulates a basic cell-cycle in vitro, by indirect immunofluorescence microscopy.
Kill, IR, Hutchison, C
core   +6 more sources

Cell-free Biosynthesis of Peptidomimetics

open access: yesBiotechnology and Bioprocess Engineering, 2023
A wide variety of peptidomimetics (peptide analogs) possessing innovative biological functions have been brought forth as therapeutic candidates through cell-free protein synthesis (CFPS) systems. A key feature of these peptidomimetic drugs is the use of non-canonical amino acid building blocks with diverse biochemical properties that expand functional
Lee, Kanghun   +5 more
openaire   +3 more sources

Preparation of Cell-free Tryptophanase [PDF]

open access: yesNature, 1947
IN our earliest studies on the tryptophanase complex1 active non-viable suspensions of E. coli were used. These had been sterilized by shaking with chloroform, 1 volume to 2 volumes of suspension. The mixture was shaken vigorously to 200 r.p.m. for 4 hours.
E A, DAWES, J, DAWSON, F C, HAPPOLD
openaire   +2 more sources

Cell-Free DNA in the Investigation of Miscarriage [PDF]

open access: yesJournal of Clinical Medicine, 2020
Approximately one in four pregnancies result in pregnancy loss, and ~50% of these miscarriages are caused by chromosomal abnormalities. Genetic investigations are recommended after three consecutive miscarriages on products of conception (POC) tissue. Cell-free DNA (cfDNA) has been utilised for prenatal screening, but very little work has been carried ...
Emily Colley   +8 more
openaire   +3 more sources

Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes [PDF]

open access: yes, 2010
The continuous progress in the structural and functional characterization of aquaporins increasingly attracts attention to study their roles in certain mammalian diseases.
Volker Dötsch   +23 more
core   +2 more sources

Cell-free (RNA) and cell-associated (DNA) HIV-1 and postnatal transmission through breastfeeding [PDF]

open access: yes, 2012
<p>Introduction - Transmission through breastfeeding remains important for mother-to-child transmission (MTCT) in resource-limited settings. We quantify the relationship between cell-free (RNA) and cell-associated (DNA) shedding of HIV-1 virus in ...
Van de Perre, P   +28 more
core   +1 more source

Artificial environments for the co-translational stabilization of cell-free expressed proteins [PDF]

open access: yes, 2013
An approach for designing individual expression environments that reduce or prevent protein aggregation and precipitation is described. Inefficient folding of difficult proteins in unfavorable translation environments can cause significant losses of ...
Volker Dötsch   +8 more
core   +2 more sources

Resource allocation for cell-free networks with reflective intelligent surfaces [PDF]

open access: yes, 2022
openThe recently commercialized fifth-generation (5G) wireless communication networks achieved many improvements, including air interface enhancement, spectrum expansion, and network intensification by several key technologies, such as massive multiple ...
RAYRI, AKRAM
core  

Cell‐free translation in reversed micelles [PDF]

open access: yesFEBS Letters, 1992
Cell‐free translation in reversed micelles (RM) of surfactants in organic solvents is demonstrated using as an example the synthesis of human interleukin‐2 by the wheat germ translation system solubilized in Brij 96 (oleyl‐poly(10)oxyethylene ether) RM in cyclohexane.
Nametkin, Sergey N.   +6 more
openaire   +2 more sources

Home - About - Disclaimer - Privacy