Results 241 to 250 of about 10,355,590 (280)
Some of the next articles are maybe not open access.
Application of cell free DNA in ART
Placenta, 2022Various biopsy and sampling methods are used for preimplantation genetic diagnosis (PGD) of embryo. This method benefits blastomer/trophectoderm biopsy to improve the clinical outcome of in vitro fertilization (IVF). However, all of these procedures are invasive and have adverse effects on embryo development.
Amin Alizadegan +10 more
openaire +2 more sources
Med
The analysis of cell-free DNA (cfDNA) has emerged as a cornerstone of minimally invasive liquid biopsies. We summarize the key advancements of the last five years-deepening insights into fundamental cfDNA biology, innovations in wet- and dry-lab approaches, and the amassment of clinical outcome data-and discuss prospects of this field for the coming ...
W H Adrian, Tsui, Y M Dennis, Lo
openaire +2 more sources
The analysis of cell-free DNA (cfDNA) has emerged as a cornerstone of minimally invasive liquid biopsies. We summarize the key advancements of the last five years-deepening insights into fundamental cfDNA biology, innovations in wet- and dry-lab approaches, and the amassment of clinical outcome data-and discuss prospects of this field for the coming ...
W H Adrian, Tsui, Y M Dennis, Lo
openaire +2 more sources
2019
Cell-free DNA (cfDNA) found in the plasma or serum is derived from multiple sources, including tumor cells. The fraction of cfDNA derived from tumor cells is termed circulating tumor DNA (ctDNA). Despite the low levels of ctDNA in normal cfDNA, advances in digital polymerase chain reaction (PCR) and next-generation sequencing (NGS) technologies have ...
Hiroyuki Yamamoto +2 more
openaire +1 more source
Cell-free DNA (cfDNA) found in the plasma or serum is derived from multiple sources, including tumor cells. The fraction of cfDNA derived from tumor cells is termed circulating tumor DNA (ctDNA). Despite the low levels of ctDNA in normal cfDNA, advances in digital polymerase chain reaction (PCR) and next-generation sequencing (NGS) technologies have ...
Hiroyuki Yamamoto +2 more
openaire +1 more source
Cell-free DNA Screening for Aneuploidy
Clinical Obstetrics & Gynecology, 2023Cell-free DNA (cfDNA) screening has high detection for the common fetal autosomal aneuploidies, but is not diagnostic. The positive predictive value should be utilized in counseling after a positive cell-free DNA screen, and diagnostic testing should be offered for confirmation.
openaire +2 more sources
Circulating cell free DNA: Preanalytical considerations
Clinica Chimica Acta, 2013Despite the growing interest in circulating cell-free DNA (ccfDNA) analysis in various clinical fields, especially oncology and prenatal diagnosis, few studies on sample handling have been reported and no analytical consensus is available. The lack of consistency between the various protocols for sample handling and the techniques used for ccfDNA ...
El Messaoudi, Safia +3 more
openaire +4 more sources
The Journal of molecular diagnostics : JMD, 2017
The analysis of cell-free DNA (cfDNA) as a sensitive biomarker for cancer diagnosis and monitoring has resulted in a need for efficient and standardized cfDNA isolation. In this study, we compared the isolation efficiency of the QIAamp circulating nucleic acid kit (QIA) with four other cfDNA isolation kits: the PME free-circulating DNA Extraction Kit ...
Sorber, Laure +6 more
openaire +3 more sources
The analysis of cell-free DNA (cfDNA) as a sensitive biomarker for cancer diagnosis and monitoring has resulted in a need for efficient and standardized cfDNA isolation. In this study, we compared the isolation efficiency of the QIAamp circulating nucleic acid kit (QIA) with four other cfDNA isolation kits: the PME free-circulating DNA Extraction Kit ...
Sorber, Laure +6 more
openaire +3 more sources
Cell-Free DNA in Hematologic Malignancies
JCO Oncology PracticeLiquid biopsy techniques using cell-free DNA (cfDNA) play an increasingly important role in the characterization and surveillance of solid tumors. For blood cancers, molecular response assessment techniques using circulating malignant cells or bone marrow aspirates are well established in clinical care. However, cfDNA has an emerging role in hematology
Joseph G. Schroers-Martin +1 more
openaire +2 more sources
Cell-Free DNA Integrity: Applications
2018Cell-free DNA integrity (cfDNAi) could be a valuable biomarker for solid tumors, to define prognosis and response to therapy. Several elements have been studied for cfDNAi, such as specific genes involved in cancer progression or repetitive DNA sequences as surrogate markers for the whole circulating DNA.However, the lack of a standardized method for ...
openaire +2 more sources
Annals of the New York Academy of Sciences, 2001
Abstract: Intrigued by the rapid clearance of free fetal DNA from the maternal circulation, we have investigated whether this fetal genetic material could be cleared via the kidney. For this purpose, we examined for the presence of Y chromosome‐specific DNA sequences in urine samples obtained from 8 women pregnant with male fetuses.
XIAO YAN ZHONG +7 more
openaire +1 more source
Abstract: Intrigued by the rapid clearance of free fetal DNA from the maternal circulation, we have investigated whether this fetal genetic material could be cleared via the kidney. For this purpose, we examined for the presence of Y chromosome‐specific DNA sequences in urine samples obtained from 8 women pregnant with male fetuses.
XIAO YAN ZHONG +7 more
openaire +1 more source
Methodological Variables in the Analysis of Cell-Free DNA
2016In recent years, cell-free DNA (cfDNA) analysis has received increasing amounts of attention as a potential non-invasive screening tool for the early detection of genetic aberrations and a wide variety of diseases, especially cancer. However, except for some prenatal tests and BEAMing, a technique used to detect mutations in various genes of cancer ...
Bronkhorst, Abel Jacobus +2 more
openaire +3 more sources