Results 121 to 130 of about 266,442 (278)

Cytosine ribose flexibility in DNA : a combined NMR 13C spin relaxation and molecular dynamics simulation study

, 2008
Using (13)C spin relaxation NMR in combination with molecular dynamic (MD) simulations, we characterized internal motions within double-stranded DNA on the pico- to nano-second time scale.
Elke Duchardt, Nilsson, Lennart, Duchardt, Elke, Schleucher, Jürgen,, Duchardt-Ferner, Elke, Schleucher, Jürgen, Lennart Nilsson, Jürgen Schleucher   +7 more
core   +1 more source

Oppositely Charged Single Enzyme Nanogels Form Versatile Coacervates for Efficient Enzyme Cascade Catalysis

Advanced Materials, EarlyView.
Oppositely charged single enzyme nanogels (SENs) phase‐separate into bi‐enzymatic coacervate microdroplets, acting as both scaffold and functional units. By tuning SEN ratios, these coacervates create specific microenvironments that enable selective small‐molecule enrichment and efficient intermediate diffusion.
Andoni Rodriguez‐Abetxuko, Nadia A. Erkamp, Neshat Moslehi, Yannick H. A. Leurs, Lars Paffen, Işil Yeşil Gür, Jana Stojanović, Hugo Brasselet, Laura van der Weel, Ulf Hanefeld, Ilja K. Voets, Jan C. M. van Hest   +11 more
wiley   +1 more source

Intimate Interaction Between Nucleic Acid and Conjugated Polymers in Organic Electrochemical Transistors Enables Ultrasensitive Biomarker Detection

Advanced Materials, EarlyView.
The intimate interaction between negatively charged RNA molecules and conjugated polymers in organic electrochemical transistors (OECTs) in aqueous electrolytes is investigated. It demonstrates that RNA binding reduces the volumetric capacitance of the polymer channel, enabling the development of an ultrasensitive biosensing platform capable of ...
Hong Liu, Naixiang Wang, Anneng Yang, Jiajun Song, Li Li, Iain McCulloch, Helen Ka‐wai Law, Feng Yan   +7 more
wiley   +1 more source

Cell-free nucleic acid fragmentomics: A non-invasive window into cellular epigenomes

Translational Oncology
Clinical genomic profiling of cell-free nucleic acids (e.g. cell-free DNA or cfDNA) from blood and other body fluids has ushered in a new era in non-invasive diagnostics and treatment monitoring strategies for health conditions and diseases such as cancer.
Ahmad Salman Sirajee, Debajyoti Kabiraj, Subhajyoti De   +2 more
openaire   +3 more sources

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

, 2008
PCR inhibition by nucleic acid extracts is a well known yet poorly described phenomenon. Inhibition assessment generally depends on the assumption that inhibitors affect all PCR reactions to the same extent; i.e.
Miller Robert F, Morris-Jones Stephen D, Tanya Novak, Garson Jeremy A, Jim F Huggett, Morris-Jones, SD, Zumla, A, Huggett, JF, Miller, RF, Green Clare, Zumla Alimuddin, Garson, JA, Novak Tanya, Novak, T, Jeremy A Garson, Clare Green, Stephen D Morris-Jones, Robert F Miller, Green, C, Huggett Jim F, Alimuddin Zumla   +20 more
core   +1 more source

A Dual‐Bioresponsive and Programmable Microneedle Matrix as a Bioinspired Coupler for Orchestrating Diabetic Bone Regeneration

Advanced Materials, EarlyView.
This project developed a smart bandage‐like patch (a microneedle array) for repairing diabetic bone damage. It intelligently senses signals from infection and inflammation, then releases its medicines in a specific, timed sequence: first an antibacterial agent, then an anti‐inflammatory agent, and finally growth factors.
Yu Wang, Rui Chen, Yu Chen, Haowen Lu, Yuchen Qian, Zhiheng Gao, Zheming Shen, Xiaofei Zheng, Huilin Yang, Jun Zou, Yusen Qiao   +10 more
wiley   +1 more source

The Sulfolobus solfataricus radA paralogue sso0777 is DNA damage inducible and positively regulated by the Sta1 protein

, 2013
Little is known about the regulation of the DNA damage-mediated gene expression in archaea. Here we report that the addition of actinomycin D to Sulfolobus solfataricus cultures triggers the expression of the radA paralogue sso0777.
Campoy, S, Paytubi, S, White, Malcolm Frederick, Barbe, J, Rodriquez, S, Abella, M   +5 more
core   +1 more source

BioEISense: A Microfluidic Platform for Real‐Time Monitoring of Staphylococcus aureus Biofilm Formation and the Efficacy of Antibiofilm Agents

Advanced Materials Interfaces, EarlyView.
BioEISense is a microfluidic device with integrated impedance sensors, for real‐time, label‐free monitoring of S. aureus biofilms. In this study, the biofilm culture conditions were optimized to support sensitive and reproducible detection of biofilm formation and eradication under dynamic flow‐through conditions. The system was also validated for both
Jéssica Amorim, Laura Vesalainen, Loïc Dreano, Markus Haapala, Cristina D. Cruz, Päivi Tammela, Tiina M. Sikanen   +6 more
wiley   +1 more source

A multifaceted strategy for intra- and extracellular nucleic acid regulation to alleviate intervertebral disc degeneration

Nature Communications
Abnormal accumulation of both intracellular and extracellular free nucleic acids drives chronic inflammation in intervertebral disc degeneration (IVDD).
Lan Zhao, Kaiyu Liang, Wenqiang Cheng, Xizhuo Zhou, Libin Yang, Xinning Mao, Weihui Qi, Hongting Jin, Wei Zhang, Hao Pan, Dong Wang   +10 more
doaj   +1 more source

Global Trends in Research on Cell-Free Nucleic Acids in Obstetrics and Gynecology during 2017-2021. [PDF]

J Clin Med, 2022
Gao W, Yang H, Cheng W, Wang X, Li D, Shi B.   +5 more
europepmc   +1 more source