Results 181 to 190 of about 1,526,120 (295)

Cell-free protein synthesis platforms for accelerating drug discovery. [PDF]

Biotechnol Notes
Caschera F.
europepmc   +1 more source

Rapid screening and scaled manufacture of immunogenic virus-like particles in a tobacco BY-2 cell-free protein synthesis system. [PDF]

Front Immunol, 2023
Armero-Gimenez J, Wilbers R, Schots A, Williams C, Finnern R.   +4 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels. [PDF]

Adv Biol (Weinh)
Kaufmann A, Ivanova K, Thiele J.
europepmc   +1 more source

High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems. [PDF]

Methods Mol Biol, 2022
Meyer C, Zhou C, Fang Z, Longo ML, Pan T, Tan C.   +5 more
europepmc   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Digital Microfluidics-Driven Cell-Free Protein Synthesis Platform Reveals Expression and Stability Determinants for Phytoglobins and Cysteine-to-Alanine Substituted Variants. [PDF]

Antioxidants (Basel)
Groth L, Bülow L.
europepmc   +1 more source

Cell-Free Protein Synthesis with Fungal Lysates for the Rapid Production of Unspecific Peroxygenases. [PDF]

Antioxidants (Basel), 2022
Schramm M, Friedrich S, Schmidtke KU, Kiebist J, Panzer P, Kellner H, Ullrich R, Hofrichter M, Scheibner K.   +8 more
europepmc   +1 more source

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

FEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa, Saori Kagoshima, Akira Yamada   +2 more
wiley   +1 more source

Cell-Free Protein Synthesis as a Method to Rapidly Screen Machine Learning-Generated Protease Variants. [PDF]

ACS Synth Biol
Thornton EL, Boyle JT, Laohakunakorn N, Regan L.   +3 more
europepmc   +1 more source