Results 31 to 40 of about 1,526,120 (295)

Flexible programming of cell-free protein synthesis using magnetic bead-immobilized plasmids. [PDF]

PLoS ONE, 2012
The use of magnetic bead-immobilized DNA as movable template for cell-free protein synthesis has been investigated. Magnetic microbeads containing chemically conjugated plasmids were used to direct cell-free protein synthesis, so that protein generation ...
Ka-Young Lee, Kyung-Ho Lee, Ji-Woong Park, Dong-Myung Kim   +3 more
doaj   +1 more source

Cell-Free Synthesis of the Mitochondrial ADP/ATP Carrier Protein of Neurospora crassa [PDF]

, 1979
ADP/ATP carrier protein was synthesized in heterologous cell-free systems programmed with Neurospora poly(A)-containing RNA and homologous cell-free systems from Neurospora.
Allen E. H., Aviv H., Blomback B., Cassio D., Dubnoff J. S., Fraser T. H., Hackenberg H., Hallermayer G., Harmey M. A., Inouye S., Klingenberg M., Korb H., Laemmli U. K., Leaver C. J., Lodish H. F., Maccechini M.-L., Marahiel M. A., Michel R., Palmiter R. D., Pelham H. R. B., Roberts B. E., Scheele G., Sebald W., Stewart J. W., Vignais P. V., Zitomer R. S.   +25 more
core   +1 more source

Biosynthetic pathway of mitochondrial ATPase subunit 9 in Neurospora crassa [PDF]

, 1983
Subunit 9 of mitochondrial ATPase (Su9) is synthesized in reticulocyte lysates programmed with Neurospora poly A-RNA, and in a Neurospora cell free system as a precursor with a higher apparent molecular weight than the mature protein (Mr 16,400 vs.
Hennig, Bernd, Neupert, Walter, Schmidt, Bernd, Zimmermann, Richard   +3 more
core   +1 more source

Cell-Free Protein Synthesis: Chassis toward the Minimal Cell

Cells, 2019
The quest for a minimal cell not only sheds light on the fundamental principles of life but also brings great advances in related applied fields such as general biotechnology.
Ke Yue, Yiyong Zhu, Lei Kai
doaj   +1 more source

Changes in the microsomal proteome of tomato fruit during ripening [PDF]

, 2019
The variations in the membrane proteome of tomato fruit pericarp during ripening have been investigated by mass spectrometry-based label-free proteomics.
De Lorenzo, Giulia, Fabbri, Claudia, Licursi, Valerio, Mattei, Benedetta, Negri, Rodolfo, Pontiggia, Daniela, Spinelli, Francesco   +6 more
core   +1 more source

The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins

Frontiers in Bioengineering and Biotechnology, 2022
The ongoing pandemic caused by the novel coronavirus (SARS-CoV-2) has led to more than 445 million infections and the underlying disease, COVID-19, resulted in more than 6 million deaths worldwide.
Franziska Ramm, Franziska Ramm, Srujan K. Dondapati, Hoai Anh Trinh, Hoai Anh Trinh, Dana Wenzel, Ruben M. Walter, Ruben M. Walter, Anne Zemella, Stefan Kubick, Stefan Kubick, Stefan Kubick   +11 more
doaj   +1 more source

Assembly of mitochondria [PDF]

, 1980
The majority of mitochondrial proteins are synthesized on cytoplasmic ribosomes and transferred to the mitochondria where they are assembled to supramolecular structures.
Harmey, Matthew A., Neher, Eva-Maria, Neupert, Walter, Zimmermann, Richard   +3 more
core   +1 more source

Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics

Synthetic and Systems Biotechnology, 2018
High-sensitivity mass spectrometry approaches using selected reaction monitoring (SRM) or multiple reaction monitoring (MRM) methods are powerful tools for targeted quantitative proteomics-based investigation of dynamics in specific biological systems ...
Ryohei Narumi, Keiko Masuda, Takeshi Tomonaga, Jun Adachi, Hiroki R. Ueda, Yoshihiro Shimizu   +5 more
doaj   +1 more source

Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor. [PDF]

, 2016
Cellular amino acid uptake is critical for mTOR complex 1 (mTORC1) activation and cell proliferation. However, the regulation of amino acid uptake is not well-understood.
Braas, Daniel, Christofk, Heather R, Graeber, Thomas G, Krall, Abigail S, Xu, Shili   +4 more
core   +1 more source

Besnoitia besnoiti infection alters both endogenous cholesterol de novo synthesis and exogenous LDL uptake in host endothelial cells [PDF]

, 2008
Besnoitia besnoiti, an apicomplexan parasite of cattle being considered as emergent in Europe, replicates fast in host endothelial cells during acute infection and is in considerable need for energy, lipids and other building blocks for offspring ...
Silva, Liliana M. R., Lütjohann, Dieter, Hamid, Penny, Velasquez, Zahady D., Kerner, Katharina, Larrazabal, Camilo, Failing, Klaus, Hermosilla, Carlos, Taubert, Anja   +8 more
core   +2 more sources