Results 41 to 50 of about 1,526,120 (295)
Synthetic and Systems Biotechnology, 2019 An “endotoxin-free” E. coli-based cell-free protein synthesis system has been reported to produce therapeutic proteins rapidly and on-demand. However, preparation of the most complex CFPS reagent – the cell extract – remains time-consuming and labor ...
J. Porter Hunt +5 more
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Cell-free Synthesis of Pea Seed Proteins [PDF]
Plant Physiology, 1977 Both polysomes and polysomal RNA, isolated from cotyledons of ripening pea (Pisum sativum) seeds and supplemented respectively with wheat germ S-100 and S-30 fractions, were used to program the cell-free synthesis of polypeptides. The relationship of these polypeptide products to seed storage proteins has been investigated. When fractionated on sucrose
T J, Higgins, D, Spencer
openaire +2 more sources
Inhibition of eukaryotic cell-free protein synthesis by thionins from wheat endosperm [PDF]
, 1983 Thionins are polypeptide toxins of about 5000 molecular weight, present in the endosperms of many Gramineae, which modify membrane permeability and inhibit macromolecular synthesis in cultured mammalian cells.
Carbonero Zalduegui, Pilar +5 more
core +2 more sources
Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals [PDF]
The Ukrainian Biochemical Journal, 2020 The effect of penetrating (glycerol, DMSO) and poorly penetrating (PEG-400) cryoprotective agents on labeled amino acids incorporation into de novo synthesized proteins in the cells of mice thymus, lymph nodes, spleen and cell-free rat liver extract was ...
A. K. Gulevskyy +2 more
doaj +1 more source
Cell-free synthesis of measles virus proteins [PDF]
Journal of Virology, 1979 Polyadenylated mRNA extracted from cytoplasm of measles virus-infected Vero cells was translated in a cell-free system. Three of the polypeptides obtained corresponded to nucleocapsid protein, phosphoprotein, and membrane protein of measles virions. A fourth polypeptide, present in measles virus-infected cells, could be generated by addition of Vero ...
J, Sprague +3 more
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Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method
Nature CommunicationsTransfer RNA (tRNA) plays a central role in translation. The simultaneous in vitro synthesis of minimal yet sufficient tRNA species (at least 21) poses a challenge for constructing a self-reproducible artificial cell.
Ryota Miyachi +3 more
doaj +1 more source
Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of "Difficult-to-Express" Proteins and Future Perspectives. [PDF]
PLoS ONE, 2016 Nowadays, biotechnological processes play a pivotal role in target protein production. In this context, Chinese Hamster Ovary (CHO) cells are one of the most prominent cell lines for the expression of recombinant proteins and revealed as a safe host for ...
Lena Thoring +5 more
doaj +1 more source
Rational improvement of cell-free protein synthesis
New Biotechnology, 2011 Experimental design principles were applied on cell-free protein synthesis to optimize performance with regard to the expression yield and the incorporation efficiency of amino acid precursors. A versatile screening platform based on batch-mode cell-free expression and central composite design was used.
Anders, Pedersen +3 more
openaire +2 more sources