Results 81 to 90 of about 775,653 (341)

Construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering

BMC Proceedings, 2013
Dihydrofolate reductase (DHFR)-mediated gene amplification has been widely used to establish high-producing mammalian cell lines [1-3]. However, since gene amplification is an infrequent event, in that many rounds of methotrexate (MTX) selection to ...
K. Lee, K. Honda, H. Ohtake, T. Omasa
semanticscholar   +1 more source

Cancer immune control needs senescence induction by interferon-dependent cell cycle regulator pathways in tumours

Nature Communications, 2020
Immune checkpoint blockade (ICB)-based or natural cancer immune responses largely eliminate tumours. Yet, they require additional mechanisms to arrest those cancer cells that are not rejected.
E. Brenner, B. Schörg, Fatima Ahmetlić, T. Wieder, F. Hilke, N. Simon, C. Schroeder, German Demidov, Tanja Riedel, B. Fehrenbacher, M. Schaller, A. Forschner, T. Eigentler, H. Niessner, T. Sinnberg, Katharina Böhm, Nadine Hömberg, H. Braumüller, D. Dauch, Stefan Zwirner, L. Zender, D. Sonanini, Albert Geishauser, J. Bauer, M. Eichner, Katja J. Jarick, A. Beilhack, S. Biskup, D. Döcker, D. Schadendorf, L. Quintanilla‐Martinez, B. Pichler, M. Kneilling, R. Mocikat, M. Röcken   +34 more
semanticscholar   +1 more source

Cis‐regulatory and long noncoding RNA alterations in breast cancer – current insights, biomarker utility, and the critical need for functional validation

Molecular Oncology, EarlyView.
The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués, Aracele Martinez‐Mendez, John Crown, Alex Eustace   +3 more
wiley   +1 more source

Glucocorticoids and checkpoint tyrosine kinase inhibitors stimulate rat pancreatic beta cell proliferation differentially.

PLoS ONE, 2019
Cell therapy for diabetes could benefit from the identification of small-molecule compounds that increase the number of functional pancreatic beta cells.
Sarah Akbib, Jordy Stichelmans, Geert Stangé, Zhidong Ling, Zerihun Assefa, Karine H Hellemans   +5 more
doaj   +1 more source

DDK Has a Primary Role in Processing Stalled Replication Forks to Initiate Downstream Checkpoint Signaling

Neoplasia: An International Journal for Oncology Research, 2018
Summary: CDC7-DBF4 kinase (DDK) initiates DNA replication in eukaryotes by activating the replicative MCM helicase. DDK has diverse and apparently conflicting roles in the replication checkpoint response in various organisms, but the underlying ...
Nanda Kumar Sasi, Flavie Coquel, Yea-Lih Lin, Jeffrey P MacKeigan, Philippe Pasero, Michael Weinreich   +5 more
doaj   +1 more source

Detection of circulating tumor DNA in colorectal cancer patients using a methylation‐specific droplet digital PCR multiplex

Molecular Oncology, EarlyView.
We developed a cost‐effective methylation‐specific droplet digital PCR multiplex assay containing tissue‐conserved and tumor‐specific methylation markers. The assay can detect circulating tumor DNA with high accuracy in patients with localized and metastatic colorectal cancer.
Luisa Matos do Canto, Louise Raunkilde, Jan Lindebjerg, Mads Malik Aagaard, Christina Therkildsen, Jakob Kleif, Lars Henrik Jensen, Torben Frøstrup Hansen, Rikke Fredslund Andersen   +8 more
wiley   +1 more source

Exploiting metabolic adaptations to overcome dabrafenib treatment resistance in melanoma cells

Molecular Oncology, EarlyView.
We show that dabrafenib‐resistant melanoma cells undergo mitochondrial remodeling, leading to elevated respiration and ROS production balanced by stronger antioxidant defenses. This altered redox state promotes survival despite mitochondrial damage but renders resistant cells highly vulnerable to ROS‐inducing compounds such as PEITC, highlighting redox
Silvia Eller, Susanne Ebner, Carmen Haselrieder, Julia K. Günther, Astrid Drasche, Sophie Strich, Chiara Volani, Andrea Medici, Aleksandar Nikolajevic, Alex Deltedesco, Johannes E. Sigmund, Michael J. Blumer, Martin Hermann, Johanna Vanacker, Gerald Brandacher, Eduard Stefan, Omar Torres‐Quesada, Jakob Troppmair   +17 more
wiley   +1 more source

The G2 checkpoint—a node‐based molecular switch

FEBS Open Bio, 2017
Tight regulation of the eukaryotic cell cycle is paramount to ensure genomic integrity throughout life. Cell cycle checkpoints are present in each phase of the cell cycle and prevent cell cycle progression when genomic integrity is compromised.
Mark C. deGooijer, Arnout van denTop, Irena Bockaj, Jos H. Beijnen, Thomas Würdinger, Olaf vanTellingen   +5 more
doaj   +1 more source

Rb interactome data and its modulations during cell cycle progression in HEK 293 cells

Data in Brief, 2018
The Rb protein is a tumor suppressor protein that regulates the key G1S checkpoint consequently blocking the progression of cell cycle into S-phase.
Shweta Duggal, Noor Jailkhani, Mukul Kumar Midha, Kanury V.S. Rao, Ajay Kumar   +4 more
doaj   +1 more source

Cell cycle: Checkpoint proteins and kinetochores [PDF]

Current Biology, 1997
Vertebrate homologs of yeast spindle assembly checkpoint proteins are localized to kinetochores and may act as a sensor for proper chromosome attachment to the mitotic spindle.
openaire   +2 more sources