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Microcalorimetry of isolated mammalian cells
Nature, 1977USING microcalorimetry1,2, we have measured the heat evolved by isolated cells of brown adipose tissue (BAT). This tissue generates heat in newborn and cold-adapted mammals and in hibernators during arousal from hibernation and during cold stress3–5. The method can be applied generally in work with, for example, mitochondria and isolated cells and has,
J, Nedergaard, B, Cannon, O, Lindberg
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Calcium transport in isolated bone cells I. Bone cell isolation procedures
Journal of Cellular Physiology, 1974AbstractBone cells were isolated by collagenase‐hyaluronidase digestion of 19–20 day‐old fetal rat calvaria. It is shown that contamination from calcified matrix can give erroneously high values for total cell calcium and can mask intracellular calcium exchange.Filtration of the cell suspension through 35 μ mesh nylon net and a five minute treatment ...
R, Dziak, J S, Brand
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Isolation of Mouse Mononuclear Cells
Current Protocols in Immunology, 2000AbstractThis unit covers techniques for preparing lymphoid cell suspensions and removing red blood cells and dead cells. These basic manipulations are required for most of the subsequent protocols in this chapter, i.e., measuring functional responses of mouse lymphocytes.
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Effect of method of cell isolation on the metabolic activity of isolated rat liver cells
Journal of Cell Science, 1972ABSTRACT Rat liver cells isolated with a tissue press, by tetraphenylboron (TPB) chelation, or by hyaluronidase and collagenase digestion were compared as to morphology, cell yield, and biosynthetic activity. The cells were intact by light microscopy; ultrastructural changes were present on electron-microscopic examination of all cell ...
L G, Lipson, D M, Capuzzi, S, Margolis
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Isolation of Chemoresistant Cell Subpopulations
2016Chemoresistance is a major challenge for cancer therapy and drives tumor relapse. The emergence, within the treated tumor mass, of specific cancer cell subpopulations endowed with high tolerance to the microenvironment stress induced by therapy is being growingly recognized as a mechanism of tumor progression. To obtain detailed information with regard
Claudia, Canino, Mario, Cioce
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Isolation of Amniotic Mesenchymal Stem Cells
Current Protocols in Stem Cell Biology, 2010AbstractMesenchymal stem cells (MSCs) have the ability to differentiate into osteocytes, chondrocytes, and adipocytes and possess immunomodulatory properties. Amniotic membrane from human term placenta is a potential source of multipotent MSCs that could be useful for regenerative medicine.
MARONGIU, FABIO +7 more
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Isolation of Amniotic Epithelial Stem Cells
Current Protocols in Stem Cell Biology, 2010AbstractMany of the cell types that can be isolated from placental tissues retain phenotypic plasticity that makes them an interesting source of cells for regenerative medicine. Several procedures for the isolation of stem cells from different parts of the placenta have been reported. This unit describes a detailed and simple protocol for the selective
MIKI T +4 more
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Identification and Isolation of Synovial Dendritic Cells
2007In rheumatoid arthritis patients, three compartments need to be considered: peripheral blood, synovial fluid, and synovial tissue. Dendritic cells characterized from each compartment have different properties. The methods given are based on cell sorting for isolation of cells, and flow cytometry and immunohistochemical staining for analysis of cells in
Pettit, Allison R. +5 more
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Neural Stem Cell Isolation and Characterization
2006Throughout the process of development and continuing into adulthood, stem cells function as a reservoir of undifferentiated cell types, whose role is to underpin cell genesis in a variety of tissues and organs. In the adult, they play an essential homeostatic role by replacing differentiated tissue cells "worn off" by physiological turnover or lost to ...
Rietze, R. L., Reynolds, B. A.
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Isolation and Culture of Schwann Cells
2013Primarily cultured Schwann cells are essential for the investigation of molecular mechanisms regulating proliferation, survival, differentiation, and myelination of Schwann cell and for the development of efficient transplantation for regeneration of injured spinal cord or peripheral nervous system.
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