Results 211 to 220 of about 1,131,780 (265)
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Disintegration of isolated cells
Analytical Biochemistry, 1987A simple device has been developed for the disintegration of isolated cells in isotonic or hypertonic media. It is based on the extrusion of a cell suspension through a small orifice under controlled pressure. The diameter of this interchangeable orifice has to be adapted to the cell type to be disrupted.
B M, Löffler, M, Schmidt, H, Kunze
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Current Opinion in Hematology, 1995
High-dose chemotherapy with autologous hematopoietic progenitor cell support is increasingly used to treat a variety of malignant diseases. A drawback of this technique is the potential for infusing clonogenic tumor cells with the autograft, producing relapse of the disease in the patient.
E J, Shpall +9 more
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High-dose chemotherapy with autologous hematopoietic progenitor cell support is increasingly used to treat a variety of malignant diseases. A drawback of this technique is the potential for infusing clonogenic tumor cells with the autograft, producing relapse of the disease in the patient.
E J, Shpall +9 more
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Current Protocols in Immunology, 1998
AbstractThis unit presents two methods for preparing dendritic cells (DCs), a highly specialized type of antigen‐presenting cell (APC). The first method involves the isolation of DCs from mouse spleen, resulting in a cell population that is highly enriched in accessory cell and APC function.
Kayo, Inaba +4 more
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AbstractThis unit presents two methods for preparing dendritic cells (DCs), a highly specialized type of antigen‐presenting cell (APC). The first method involves the isolation of DCs from mouse spleen, resulting in a cell population that is highly enriched in accessory cell and APC function.
Kayo, Inaba +4 more
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Current Protocols in Immunology, 2009
AbstractThis unit presents two methods for preparing dendritic cells (DCs), a highly specialized type of antigen‐presenting cell (APC). The first method involves the isolation of DCs from mouse spleen, resulting in a cell population that is highly enriched in accessory cell and APC function.
Kayo, Inaba +5 more
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AbstractThis unit presents two methods for preparing dendritic cells (DCs), a highly specialized type of antigen‐presenting cell (APC). The first method involves the isolation of DCs from mouse spleen, resulting in a cell population that is highly enriched in accessory cell and APC function.
Kayo, Inaba +5 more
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2016
This chapter describes a method allowing the purification of the cell wall for studying both polysaccharides and proteins. The plant primary cell wall is mainly composed of polysaccharides (90-95 % in mass) and of proteins (5-10 %). At the end of growth, specialized cells may synthesize a lignified secondary wall composed of polysaccharides (about 65 %)
Hervé, Canut +2 more
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This chapter describes a method allowing the purification of the cell wall for studying both polysaccharides and proteins. The plant primary cell wall is mainly composed of polysaccharides (90-95 % in mass) and of proteins (5-10 %). At the end of growth, specialized cells may synthesize a lignified secondary wall composed of polysaccharides (about 65 %)
Hervé, Canut +2 more
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Isolation of Intact Liver Cells
Nature, 1957LIVER cells have been isolated by various methods, such as by forcing the tissue through stainless steel sieves1, by tryptic digestion2 and by incubation in acid solutions3. Anderson4 obtained a high yield of cells by perfusion of liver with calcium-free solutions containing chelating agents (such as ethylenediamine tetraacetate (versene) or citrate ...
M V, BRANSTER, R K, MORTON
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Perifusion system for isolated cells
Analytical Biochemistry, 1981Abstract A system is described for perifusion of isolated hepatic cells which has a small interior space, rapidly responds to substrate changes, and requires no mechanical agitation of the cells. A 22 × 2-mm circular chamber is used with membranes (25 mm diameter, 8 μm pore size) at top and bottom.
R H, McMenamy +3 more
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Metabolism of Isolated Liver Cells
Nature, 1956Longmuir and ap Rees1 have recently described a new method for preparing suspensions of isolated rat liver cells. We have been interested for some time in the metabolic properties of such isolated cells, and wish to direct attention to some of their peculiarities not mentioned by these authors.
J O, LAWS, L H, STRICKLAND
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Isolation of Mast Cell Granules
Current Protocols in Cell Biology, 2005AbstractThe mast cell is a multipotent inflammatory cell that has been shown to participate in the pathogenesis of a variety of diseases, such as immediate hypersensitivity reactions, arthritis, atherosclerosis, and heart failure. Upon stimulation, mast cells exocytose cytoplasmic secretory granules into their extracellular microenvironment.
Ken A, Lindstedt, Petri T, Kovanen
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