Results 141 to 150 of about 845,975 (261)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

BLoss-DDNet: bending loss and dual-task decoding network for overlapping cell nucleus segmentation of cervical clinical LBC images. [PDF]

Front Artif Intell
Yang G, Chen Z, Guo P, Ma J, Huang J, Jin C, Yang X, Zhao K, Wang Y, Gao Q, Liu C, Wu T, Li Y, Guo Y, Zheng J, Cai X, Yang Y.   +16 more
europepmc   +1 more source

Mechanical Frustration of Phase Separation in the Cell Nucleus by Chromatin. [PDF]

Phys Rev Lett, 2021
Zhang Y, Lee DSW, Meir Y, Brangwynne CP, Wingreen NS.   +4 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Precision and Accuracy in Quantitative Measurement of Gene Expression from Single-cell/nucleus RNA Sequencing Data. [PDF]

Genomics Proteomics Bioinformatics
Dai R, Zhang M, Chu T, Kopp R, Zhang C, Liu K, Wang Y, Wang X, Chen C, Liu C.   +9 more
europepmc   +1 more source

Modeling stem cell nucleus mechanics using confocal microscopy. [PDF]

Biomech Model Mechanobiol, 2021
Kennedy Z, Newberg J, Goelzer M, Judex S, Fitzpatrick CK, Uzer G.   +5 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Microtopography-induced changes in cell nucleus morphology enhance bone regeneration by modulating the cellular secretome. [PDF]

Nat Commun
Wang X, Li Y, Lin Z, Pla I, Gajjela R, Mattamana BB, Joshi M, Liu Y, Wang H, Zun AB, Wang H, Wai CM, Agrawal V, Dunton CL, Duan C, Jiang B, Backman V, He TC, Reid RR, Luo Y, Ameer GA.   +20 more
europepmc   +1 more source

The effects of measurement parameters on the cancerous cell nucleus characterisation by atomic force microscopy in vitro. [PDF]

J Microsc, 2022
Zhu J, Tian Y, Yan J, Hu J, Wang Z, Liu X.   +5 more
europepmc   +1 more source

Aging Is a Key Driver for Adult Acute Myeloid Leukemia

Aging and Cancer, EarlyView.
Acute myeloid leukemia (AML) is a classical age‐related hematologic malignancy, and a key driver of AML is aging, which profoundly regulates intrinsic factors such as genomic instability, epigenetic reprogramming, and metabolic dysregulation, and alters bone marrow microenvironment.
Rong Yin, Haojian Zhang
wiley   +1 more source