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Fluorescence Activated Cell Sorting
Review of Scientific Instruments, 1972An instrument has been developed for sorting biological cells. The cells are rendered differentially fluorescent and incorporated into a small liquid stream illuminated by a laser beam. The cells pass sequentially through the beam, and fluorescent light from the cells gives rise to electrical signals.
W A, Bonner +3 more
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Ultrathroughput immunomagnetic cell sorting platform
Lab on a Chip, 2022High-throughput phenotypic cell sorting is critical to the development of cell-based therapies and cell screening discovery platforms.
David N. Philpott +6 more
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2014
Single-cell Raman spectroscopy is a noninvasive and label-free technology for biochemical analysis of bacterial cells. A single-cell Raman spectrum functions as a metabolic "fingerprint" of an individual cell, which enables differentiation of cell types, physiological states, nutrient condition, and variable phenotypes.
Mengqiu, Li +3 more
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Single-cell Raman spectroscopy is a noninvasive and label-free technology for biochemical analysis of bacterial cells. A single-cell Raman spectrum functions as a metabolic "fingerprint" of an individual cell, which enables differentiation of cell types, physiological states, nutrient condition, and variable phenotypes.
Mengqiu, Li +3 more
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Current Protocols in Cytometry, 1997
AbstractA flow cytometer has the ability to analyze several thousand cells per second. This is particularly valuable for the detection of rare events. For many biological and clinical applications, cells must be isolated on the basis of multiple quantitative properties.
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AbstractA flow cytometer has the ability to analyze several thousand cells per second. This is particularly valuable for the detection of rare events. For many biological and clinical applications, cells must be isolated on the basis of multiple quantitative properties.
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2011
During the development of multicellular organisms, cell fate specification is followed by the sorting of different cell types into distinct domains from where the different tissues and organs are formed. Cell sorting involves both the segregation of a mixed population of cells with different fates and properties into distinct domains, and the active ...
S F Gabby, Krens +1 more
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During the development of multicellular organisms, cell fate specification is followed by the sorting of different cell types into distinct domains from where the different tissues and organs are formed. Cell sorting involves both the segregation of a mixed population of cells with different fates and properties into distinct domains, and the active ...
S F Gabby, Krens +1 more
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Sophistications of cell sorting
Nature Cell Biology, 2008The self-sorting of early embryonic cells is mediated not only by pure differential adhesion but also involves other processes. Direct force measurements reveal the role of cell-cortical tension, whereas epithelial-wrapping dominates the sorting of enclosed mesenchymal cells.
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Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting
2012The sex of mammalian offspring can be predetermined by flow sorting relatively pure living populations of X- and Y-chromosome-bearing sperm. This method is based on precise staining of the DNA of sperm with the nucleic acid-specific fluorophore, Hoechst 33342, to differentiate between the subpopulations of X- and Y-sperm.
Duane L, Garner +2 more
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Microfluidic chips for cell sorting
Frontiers in Bioscience, 2008Micro total analysis systems (microTAS) also referred to as "lab-on-a-chip" is one of the fastest progressing fields in biological and chemical analyses. In recent years, microTAS for single cell analysis has drawn the attention of researchers due to its significant advantages over traditional methods for single cell manipulation, fast cell sorting and
Pu, Chen +3 more
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Practicalities of Cell Sorting
Cell sorting is a technique commonly used in academic and biotechnology laboratories in order to separate out cells or particles of interest from heterogeneous populations. Cell sorters use the same principles as flow cytometry analyzers, but instead of cell populations passing to the waste of the instrument, they can be collected for further studies ...Cheetham, Mark +5 more
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Dielectrophoretic Cell Sorting
1986Living cells can be sorted with the aid of dielectrophoresis (DEP). The method depends upon the natural differences in the effective dielectric constants of the several types (species, physiological states, etc.) being handled. The use of additive stains or chemical modifiers or other tagging agents is not usually required.
J. Kent Pollock, Herbert A. Pohl
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