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DNA Transformation of Mammalian Cells
2003The manipulation of gene sequences between cells is a fundamental technique in genetics. Mammalian cells will take up and express genes when they are exposed to either metaphase chromosomes or naked genomic or recombinant DNA. In each case the uptake and expression is enhanced by the formation of a DNA-calcium phosphate precipitate (1,2). Alternatively,
J W, Pollard +3 more
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La Revue du praticien, 1996
The use of in vitro cell transformation, as a complement to the direct study of tumors and established tumor cell lines, has enabled the analysis of the contribution of diverse genes to the tumor phenotype. The most recent results have underlined the importance of fundamental mechanisms regulating cell proliferation.
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The use of in vitro cell transformation, as a complement to the direct study of tumors and established tumor cell lines, has enabled the analysis of the contribution of diverse genes to the tumor phenotype. The most recent results have underlined the importance of fundamental mechanisms regulating cell proliferation.
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Formation of larger cell aggregates by transformed cells: an in vitro index of cell transformation.
Journal of the National Cancer Institute, 1977Virus-transformed rat and hamster embryonic cells formed larger cell aggregates than those formed by normal counterpart cells within 1-3 days. This aggregate property correlated with growth in soft agar and tumorigenicity.
A F, Steuer, R C, Ting
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Small cell lung cancer transformation: From pathogenesis to treatment
Seminars in Cancer Biology, 2022Yueyi Li, Hang Wang, Yuling Luo
exaly
Malignant Transformation of Cells in Vitro
1965Publisher Summary This chapter reviews the occurrence in vitro of malignant transformation, both spontaneous and induced, and associated morphological and physiological changes. Under the various culture conditions used spontaneous malignant transformation is a reproducible phenomenon occurring in cells from many tissues and species.
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