Results 91 to 100 of about 121,160 (299)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Towards the structural characterization of proteins involved in peptidoglycan biosynthesis [PDF]

, 2015
The cell wall is an essential structure for bacterial survival and unique to bacteria. It is responsible for maintenance of cellular shape and allows the bacterium to withstand high differences in osmotic pressure between the inner and outer leaflet of ...
Sub Membrane Biochemistry & Biophysics, Nikolaidis, I., Membrane Biochemistry and Biophysics   +2 more
core  

Actin-microtubule interplay coordinates spindle assembly in human oocytes

Nature Communications, 2019
Actin and microtubules contribute to successful oocyte maturation, but if and how these two networks communicate in human oocytes is unclear. Here the authors show that actin-microtubule interactions are essential for correct segregation of human nuclear
Johannes Roeles, Georgios Tsiavaliaris
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Leveraging current steering and the biophysics of spike generation for cellular-resolution electrical stimulation of neurons [PDF]

Summary: Electrical stimulation at cellular resolution to restore the function of neural circuits is limited by the density of available electrode arrays.
Jeff B. Brown, Nishal P. Shah, Pawel Hottowy, Alexander Sher, Alex R. Gogliettino, A.J. Phillips, Madeline R. Hays, Subhasish Mitra, Praful K. Vasireddy, Claire Baum, Ramandeep S. Vilkhu, Alan M. Litke, Amrith Lotlikar, E.J. Chichilnisky, Aviv Sharon, Ethan J. Kato   +15 more
core   +1 more source

Light‐Gated Assembly of Membrane‐Bound DNA Filaments as Synthetic Cell Modules

Small Structures
Precise spatiotemporal control over biomolecular assembly is a central requirement for engineering synthetic cells and programmable biomaterials. Of particular interest are inducible filaments that can confer stability or mechanical transformation to ...
Svetozar Gavrilović, Marco Halbeisen, Thuy An Nguyen, Yusuf Qutbuddin, Petra Schwille   +4 more
doaj   +1 more source

The Presynaptic v-ATPase Reversibly Disassembles and Thereby Modulates Exocytosis but Is Not Part of the Fusion Machinery

Cell Reports, 2017
Vacuolar H+-ATPase (v-ATPase) is a multi-subunit complex comprising two domains: the cytosolic V1 domain catalyzing ATP hydrolysis and the membranous V0 sector translocating protons across membranes.
Anna Bodzęta, Martin Kahms, Jürgen Klingauf   +2 more
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Energetics of the microsporidian polar tube invasion machinery

eLife
Microsporidia are eukaryotic, obligate intracellular parasites that infect a wide range of hosts, leading to health and economic burdens worldwide.
Ray Chang, Ari Davydov, Pattana Jaroenlak, Breane Budaitis, Damian C Ekiert, Gira Bhabha, Manu Prakash   +6 more
doaj   +1 more source