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Carbohydrate Depolymerization by Intricate Cellulosomal Systems
2017Cellulosomes are multi-enzymatic nanomachines that have been fine-tuned through evolution to efficiently deconstruct plant biomass. Integration of cellulosomal components occurs via highly ordered protein-protein interactions between the various enzyme-borne dockerin modules and the multiple copies of the cohesin modules located on the scaffoldin ...
Johanna, Stern +4 more
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The Cellulosome of Clostridium thermocellum
1988Publisher Summary The accelerated interest in microbial cellulases stems from their potential industrial application. This area is an excellent example in which basic and applied science are closely interlinked: the better understood the mechanism of cellulase action; the better can be the applicative value as a biotechnological process. This chapter
Raphael Lamed, Edward A. Bayer
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Insights into enhanced thermostability of a cellulosomal enzyme
Carbohydrate Research, 2014Improved stability of cellulosomal enzymes is of great significance in order to provide efficient degradation of cellulosic derivatives for production of biofuels. In previous reports, we created a quadruple mutant of the endoglucanase Cel8A from Clostridium thermocellum resulting from a combination of both random error-prone PCR and a bioinformatics ...
Johanna Stern +4 more
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The cellulosome and cellulose degradation by anaerobic bacteria
Applied Microbiology and Biotechnology, 2001Despite its simple chemical composition, cellulose exists in a number of crystalline and amorphous topologies. Its insolubility and heterogeneity makes native cellulose a recalcitrant substrate for enzymatic hydrolysis. Microorganisms meet this challenge with the aid of a multi-enzyme system. Aerobic bacteria produce numerous individual, extra-cellular
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Cellulosomes from Clostridium thermocellum
1988Publisher Summary Biomass in the form of cellulose is the major constituent of plant matter, thereby comprising the earth's most abundant natural organic resource. Although the repeating unit of this biopolymer is a simple disaccharide (cellobiose), cellulose fibrils are organized into a complicated paracrystalline state.
Raphael Lamed, Edward A. Bayer
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Cellulosomes of Anaerobic Fungi
2006Item does not contain ...
Ljungedahl, L.G. +5 more
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The cellulosome of Clostridium thermocellum
Biochemical Society Transactions, 1998P, Béguin, P M, Alzari
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Force and affinity in cellulosomal complexes
2019In dieser Arbeit werden die molekularen Mechanismen der Organisation des Cellulosoms - ein komplexes extrazelluläres Proteinnetzwerk - als Modellsystem für Protein-Protein Interaktionen mittels biophysikalischer Methoden untersucht. Dieses extrazelluläre Organell ermöglicht bestimmten Bakterien die Zersetzung von Cellulose, indem es Enzyme und ...
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