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Deconstruction by C. thermocellum —from microbe mediated to dynamic redistribution of cellulosomes [PDF]

open access: yesLife Science Alliance
By combining super-resolution imaging and machine learning to study the dynamics of biomass deconstruction by C. thermocellum , we show that this deconstruction goes from a microbe-mediated strategy to a dynamic redistribution of cellulosomes during ...
John M Yarbrough   +9 more
doaj   +2 more sources

Lignocellulose degradation in bacteria and fungi: cellulosomes and industrial relevance [PDF]

open access: yesFrontiers in Microbiology
Lignocellulose biomass is one of the most abundant resources for sustainable biofuels. However, scaling up the biomass-to-biofuels conversion process for widespread usage is still pending.
Kuan-Ting Hsin   +12 more
doaj   +2 more sources

A genomic analysis reveals the diversity of cellulosome displaying bacteria [PDF]

open access: yesFrontiers in Microbiology
IntroductionSeveral species of cellulolytic bacteria display cellulosomes, massive multi-cellulase containing complexes that degrade lignocellulosic plant biomass (LCB). A greater understanding of cellulosome structure and enzyme content could facilitate
Christine M. Minor   +10 more
doaj   +2 more sources

Designing chimeric enzymes inspired by fungal cellulosomes

open access: yesSynthetic and Systems Biotechnology, 2020
Cellulosomes are synthesized by anaerobic bacteria and fungi to degrade lignocellulose via synergistic action of multiple enzymes fused to a protein scaffold.
Sean P Gilmore   +2 more
exaly   +3 more sources

Functional characterization of a dockerin-containing expansin-like protein from the anaerobic fungus Neocallimastix californiae [PDF]

open access: yesBiotechnology for Biofuels and Bioproducts
Background Anaerobic microbes produce multienzyme complexes known as cellulosomes to enhance the degradation of cellulosic substrates. These complexes localize diverse enzymes onto a protein scaffold, where proteins are anchored by dockerin domains ...
Taru Koitto   +5 more
doaj   +2 more sources

Structural and functional insights into cellulosomes: masters of plant cell wall degradation [PDF]

open access: yesFrontiers in Microbiology
Cellulosomes are complex multi-enzyme systems that enable efficient cellulose breakdown in some anaerobic bacteria and fungi. Understanding cellulosome functionality plays a crucial role in expanding their potential for industrial plant biomass ...
Nataša Lindič, Maša Vodovnik
doaj   +2 more sources

Cellulolytic and hemicellulolytic capacity of Acetivibrio clariflavus [PDF]

open access: yesApplied Microbiology and Biotechnology
Lignocellulosic biomass represents the largest available renewable source of carbon. It is a recalcitrant mixture of different polysaccharides and lignin.
Katarína Šuchová, Vladimír Puchart
doaj   +2 more sources

Single-cell analysis of yeast surface display for designer cellulosome applications using a fluorescent protein complex [PDF]

open access: yesMicrobiology Spectrum
Designer cellulosomes (DCs) are precisely engineered multi-enzyme complexes aimed at lignocellulose saccharification. Achieving spontaneous designer cellulosome display on yeast cell surfaces has been a long-term objective to enhance consolidated ...
Babette Lamote   +8 more
doaj   +2 more sources

Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow [PDF]

open access: yesBiotechnology for Biofuels and Bioproducts, 2022
Background Primary degraders of polysaccharides play a key role in anaerobic biotopes, where plant cell wall accumulates, providing extracellular enzymes to release fermentable carbohydrates to fuel themselves and other non-degrader species ...
Nian Liu   +6 more
doaj   +2 more sources

Editorial: Recent advances in cellulosomes and their application in bioenergy production [PDF]

open access: yesFrontiers in Microbiology
Marimuthu Anandharaj   +3 more
doaj   +2 more sources

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