Deconstruction of Lignocellulose into Soluble Sugars by Native and Designer Cellulosomes
mBio, 2012 Lignocellulosic biomass, the most abundant polymer on Earth, is typically composed of three major constituents: cellulose, hemicellulose, and lignin. The crystallinity of cellulose, hydrophobicity of lignin, and encapsulation of cellulose by the lignin ...
Sarah Moraïs +8 more
doaj +1 more source
Ruminococcus flavefaciens 007C cellulosomes and cellulase consortium
Acta Agriculturae Slovenica, 2014 Ruminococcus flavefaciens is among the most important cellulolytic bacterial species in rumen and gastrointestinal tract of monogastric herbivorous animals. Its efficiency in degradation of (hemi)cellulosic substrates is associated with the production of
Maša VODOVNIK
doaj +1 more source
Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme
Microorganisms, 2017 The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides.
Bareket Dassa +6 more
doaj +1 more source
Synergistic interaction of the cellulosome integrating protein (CipA) from Clostridium thermocellum with a cellulosomal endoglucanase [PDF]
FEBS Letters, 1998 Activity of a cellulosomal endoglucanase (endoglucanase E; EGE) from Clostridium thermocellum against two crystalline forms of cellulose was enhanced by combination with the cellulosome integrating protein (CipA), but CipA did not enhance EGE activity against amorphous cellulose, even though it was able to bind to it.
Ciruela, Antonio +3 more
openaire +2 more sources
mBio, 2018 Cellulosomes are multienzyme complexes produced by anaerobic, cellulolytic bacteria for highly efficient breakdown of plant cell wall polysaccharides.
Lior Artzi +6 more
doaj +1 more source
Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin–dockerin interactions [PDF]
PeerJ, 2014 Cellulosic waste represents a significant and underutilized carbon source for the biofuel industry. Owing to the recalcitrance of crystalline cellulose to enzymatic degradation, it is necessary to design economical methods of liberating the fermentable ...
Yuval Hamberg +8 more
doaj +2 more sources
Towards Designer Cellulosomes in Clostridia: Mannanase Enrichment of the Cellulosomes Produced by Clostridium cellulolyticum [PDF]
Journal of Bacteriology, 2004 ABSTRACT The man5K gene of Clostridium cellulolyticum was cloned and overexpressed in Escherichia coli . This gene encodes a 424-amino-acid preprotein composed of an N-terminal leader peptide, followed by a dockerin module and a C-terminal catalytic module ...
Stéphanie, Perret +4 more
openaire +2 more sources
Cellulosomal expansin: functionality and incorporation into the complex [PDF]
Biotechnology for Biofuels, 2016 Expansins are relatively small proteins that lack enzymatic activity and are found in plants and microorganisms. The function of these proteins is to disrupt the plant cell walls by interfering with the non-covalent interchain bonding of the polysaccharides.
Lior Artzi +3 more
openaire +2 more sources
Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature
mBio, 2016 Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes. The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
Johanna Stern +3 more
doaj +1 more source
Current challenges in designer cellulosome engineering
Applied Microbiology and Biotechnology, 2023 Designer cellulosomes (DCs) are engineered multi-enzyme complexes, comprising carbohydrate-active enzymes attached to a common backbone, the scaffoldin, via high-affinity cohesin-dockerin interactions. The use of DCs in the degradation of renewable biomass polymers is a promising approach for biorefineries. Indeed, DCs have shown significant hydrolytic
Babette Lamote +5 more
openaire +3 more sources