Results 51 to 60 of about 1,723 (195)

Cellulase-Xylanase Synergy in Designer Cellulosomes for Enhanced Degradation of a Complex Cellulosic Substrate

mBio, 2010
Designer cellulosomes are precision-engineered multienzyme complexes in which the molecular architecture and enzyme content are exquisitely controlled.
Sarah Moraïs, Yoav Barak, Jonathan Caspi, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, Edward A. Bayer   +7 more
doaj   +1 more source

Ultrastable cellulosome-adhesion complex tightens under load [PDF]

Nature Communications, 2014
AbstractChallenging environments have guided nature in the development of ultrastable protein complexes. Specialized bacteria produce discrete multi-component protein networks called cellulosomes to effectively digest lignocellulosic biomass. While network assembly is enabled by protein interactions with commonplace affinities, we show that certain ...
Schoeler, Constantin, Malinowska, Klara H., Bernardi, Rafael C., Milles, Lukas F., Jobst, Markus A., Durner, Ellis, Ott, Wolfgang, Fried, Daniel B., Bayer, Edward A., Schulten, Klaus, Gaub, Hermann E., Nash, Michael A.   +11 more
openaire   +4 more sources

Impact of scaffoldin mechanostability on cellulosomal activity

Biomaterials Science, 2020
A low mechanostability cohesin between the two cellulosomal anchoring points lowers saccharification activity (top), in contrast to alternative configurations (bottom).
Albert Galera-Prat, Andrés M. Vera, Sarah Moraïs, Yael Vazana, Edward A. Bayer, Mariano Carrión-Vázquez   +5 more
openaire   +4 more sources

Grape Stalks Valorization towards Circular Economy: A Cascade Biorefinery Strategy

ChemSusChem, Volume 18, Issue 10, May 19, 2025.
A cascade protocol for grape stalks valorization has been proposed, characterized by a multidisciplinary strategy using unconventional techniques and subcritical water as a green solvent, where physical/chemical treatments synergistically interact with biological treatments to obtain added value products from all the biomass components.
Carlotta Valle, Giorgio Grillo, Emanuela Calcio Gaudino, Paola Ponsetto, Roberto Mazzoli, Giulia Bonavita, Pietro Vitale, Enrica Pessione, Emilia Garcia‐Moruno, Antonella Costantini, Giancarlo Cravotto, Silvia Tabasso   +11 more
wiley   +1 more source

From pomiculture waste to biotechnological raw material: efficient transformation using ligninosomes and cellulosomes from Pleurotus spp.

Bioresources and Bioprocessing, 2022
The goal of this study was to determine the capacity of Pleurotus spp. lignocellulosome to transform frequent pomiculture residues (grapevine-, plum-, and raspberry sawdust) into raw materials for biotechnological processes.
Jasmina Ćilerdžić, Milica Galić, Mirjana Stajić   +2 more
doaj   +1 more source

Ruminococcal cellulosome systems from rumen to human [PDF]

Environmental Microbiology, 2015
Summary A cellulolytic fiber‐degrading bacterium, R uminococcus champanellensis , was isolated from human faecal samples, and its genome was recently sequenced. Bioinformatic analysis of the
Ben David, Yonit, Dassa, Bareket, Borovok, Ilya, Lamed, Raphael, Koropatkin, Nicole, Martens, Eric, White, Bryan, Bernalier, Annick, Duncan, Sylvia, Flint, Harry, Bayer, Edward, Moraïs, Sarah   +11 more
openaire   +4 more sources

Magnetizing Biotech–Advances in (In Vivo) Magnetic Enzyme Immobilization

Engineering in Life Sciences, Volume 25, Issue 3, March 2025.
ABSTRACT Industrial biocatalysis, a multibillion dollar industry, relies on the selectivity and efficacy of enzymes for efficient chemical transformations. However, enzymes, evolutionary adapted to mild biological conditions, often struggle in industrial processes that require harsh reaction conditions, resulting in reduced stability and activity ...
Gizem Ölçücü, Karl‐Erich Jaeger, Ulrich Krauss   +2 more
wiley   +1 more source

Assembly of Xylanases into Designer Cellulosomes Promotes Efficient Hydrolysis of the Xylan Component of a Natural Recalcitrant Cellulosic Substrate

mBio, 2011
In nature, the complex composition and structure of the plant cell wall pose a barrier to enzymatic degradation. Nevertheless, some anaerobic bacteria have evolved for this purpose an intriguing, highly efficient multienzyme complex, the cellulosome ...
Sarah Moraïs, Yoav Barak, Yitzhak Hadar, David B. Wilson, Yuval Shoham, Raphael Lamed, Edward A. Bayer   +6 more
doaj   +1 more source

Extracytoplasmic polysaccharides control cellulosomal and non-cellulosomal systems in Herbivorax saccincola A7

Applied Microbiology and Biotechnology
Herbivorax saccincola A7 is an anaerobic alkali-thermophilic lignocellulolytic bacterium that possesses a cellulosome and high xylan degradation ability. To understand the expression profile of extracellular enzymes by carbon sources, quantitative real-time PCR was performed on all cellulosomal and non-cellulosomal enzyme genes of H.
Sirilak Baramee, Pakinee Thianheng, Ayaka Uke, Pattsarun Cheawchanlertfa, Chakrit Tachaapaikoon, Rattiya Waeonukul, Patthra Pason, Khanok Ratanakhanokchai, Ya-Jun Liu, Akihiko Kosugi   +9 more
openaire   +2 more sources

Diversity and Vertical Distribution of Planctomycetota in the Water Column of the Remote North Pacific

Environmental Microbiology Reports, Volume 17, Issue 1, February 2025.
In the remote regions of the Pacific Ocean, Planctomycetota diversity predominantly occupies deeper water layers ranging from 175 to 500 m. Phylogenetic analyses have identified an entire range of previously unknown genera within this group of bacteria.
Inês Rosado Vitorino, Nicola Gambardella, Miguel Semedo, Catarina Magalhães, Olga Maria Lage   +4 more
wiley   +1 more source