Results 31 to 40 of about 380,843 (313)

Biogenesis of Glyoxysomes [PDF]

, 1980
Biosynthesis of isocitrate lyase, a tetrameric enzyme of the glyoxysomal matrix, was studied in Neurospora crassa, in which the formation of glyoxysomes was induced by a substitution of sucrose medium by acetate medium. * 1.
Neupert, Walter, Zimmermann, Richard
core   +1 more source

Elimination of bacteria from semen using a combination of density gradient centrifugation and swim-up

Journal of Men's Health, 2022
Background: To evaluate the efficiency of bacterial clearance of different sperm preparation procedure. Methods: A total of 46 semen samples were collected in this study, each semen sample was divided into four equal parts (0.5 mL each).
Zhiheng Chen, Lingxiao Jiang, Hui Wang, Wumin Quan, Li Yang, Chunquan Ou, Ling Sun   +6 more
doaj   +1 more source

Motility fractionation of bacteria by centrifugation

, 2013
Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having different motility. To
Di Leonardo, Roberto, Lepore, Alessia, Maggi, Claudio, Rizzo, Alessandro, Solari, Jacopo   +4 more
core   +1 more source

Timing of semen cryopreservation: before or after processing? [PDF]

Revista Brasileira de Ginecologia e Obstetrícia
Objective: Seminal cryopreservation causes significant damage to the sperm; therefore, different methods of cryopreservation have been studied. The aim of the study was to compare the effects of density gradient processing and washing/centrifugation with
Ana Paula de Souza Kussler, Ivan Cunha Bustamante Filho, Elisa Negri, Edison Capp, Helena von Eye Corleta   +4 more
doaj   +1 more source

Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation

BMC Research Notes, 2023
Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry.
Rea Victoria P. Anunciado-Koza, Anyonya R. Guntur, Calvin P. Vary, Carlos A. Gartner, Madeleine Nowak, Robert A. Koza   +5 more
doaj   +1 more source

Circular DNA's from HeLa cell nuclei and mitochondria [PDF]

, 1970
An electron microscopic observation was made on the DNA's extracted from purified HeLa cell nuclei, mitochondria, and the whole cell, and fractionated by ethidium bromide-cesium chloride density gradient method or sucrose density gradient method. Nuclear
Hirata, Seiichi, Nishida, Shigeru, Oda, Takuzo, Omura, Sachiko, Yamamoto, Shinichiro   +4 more
core   +1 more source

Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing

Reproductive Medicine and Biology
Purpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study.
Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao   +15 more
doaj   +1 more source

IMPACT OF PREPARATION USING CONVENTIONAL AND MODIFIED DENSITY GRADIENT CENTRIFUGATION METHODS ON SPERM CONCENTRATION, MOTILITY AND NUMBER OF NORMAL MOTILE SPERM RECOVERY (NMSR)

Folia Medica Indonesiana, 2017
Some preparation techniques, either conventional or advanced, have been provided. Advanced technique may overcome the limitations of conventional techniques.
Rina Yudiwati, MPBD Pramesti, Agustinus Agustinus, E Pradana, Bambang Purwanto   +4 more
doaj   +1 more source

Stable isotope probing: Technical considerations when resolving ¹⁵N-labeled RNA in gradients [PDF]

, 2010
RNA based stable isotope probing (SIP) facilitates the detection and identification of active members of microbial populations that are involved in the assimilation of an isotopically labeled compound.
Addison, Sarah Louise, Lloyd-Jones, Gareth, McDonald, Ian R.   +2 more
core   +2 more sources

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source