Strict coupling between CFTR's catalytic cycle and gating of its Cl- ion pore revealed by distributions of open channel burst durations [PDF]
, 2010 CFTR, the ABC protein defective in cystic fibrosis, functions as an anion channel. Once phosphorylated by protein kinase A, a CFTR channel is opened and closed by events at its two cytosolic nucleotide binding domains (NBDs).
Gadsby, David C. +5 more
core +1 more source
Hydrophobic gating in BK channels [PDF]
Nature Communications, 2018 AbstractThe gating mechanism of transmembrane ion channels is crucial for understanding how these proteins control ion flow across membranes in various physiological processes. Big potassium (BK) channels are particularly interesting with large single-channel conductance and dual regulation by membrane voltage and intracellular Ca2+.
Zhiguang Jia +4 more
openaire +4 more sources
Flexibility of the N-Terminal mVDAC1 Segment Controls the Channel’s Gating Behavior [PDF]
, 2012 Since the solution of the molecular structures of members of the voltage dependent anion channels (VDACs), the N-terminal α-helix has been the main focus of attention, since its strategic location, in combination with its putative conformational ...
Grosse, Wolfgang +23 more
core +2 more sources
Molecular mechanisms of the coupling of gating to voltage sensing in transmembrane proteins [PDF]
, 2014 Voltage gated potassium ion (Kv) channels regulate action potentials of the nervous system by responding to changes in transmembrane voltage, enabling K+ transport across the membrane to restore cells to their resting potential.
Starek, Greg
core +1 more source
M-Channel Gating and Simulation [PDF]
Biophysical Journal, 1999 Single potassium M-channels in rat sympathetic neurons have multiple voltage-dependent kinetic components in their activity: short, medium, and long closed times (tau(CS), tau(CM), and tau(CL)) and short and long open times (tau(OS) and tau(OL)). All five components can be detected in cell-attached patches, but only four of them (tau(CS), tau(CM), tau ...
Selyanko, A.A., Brown, D.A.
openaire +2 more sources
Glutathione accelerates sodium channel inactivation in excised rat axonal membrane patches [PDF]
, 1992 The effects of glutathione were studied on the gating behaviour of sodium channels in membrane patches of rat axons. Depolarizing pulses from –120 to –40 mV elicited sodium currents of up to 500 pA, indicating the simultaneous activation of up to 250 ...
Quasthoff, Stefan +3 more
core +1 more source
Artificial modulation of the gating behavior of a K+ channel in a KvAP-DNA chimera. [PDF]
PLoS ONE, 2011 We present experiments where the gating behavior of a voltage-gated ion channel is modulated by artificial ligand binding. We construct a channel-DNA chimera with the KvAP potassium channel reconstituted in an artificial membrane.
Andrew Wang, Giovanni Zocchi
doaj +1 more source
The gates of ion channels and enzymes [PDF]
Trends in Biochemical Sciences, 2010 Protein dynamics are essential for virtually all protein functions, certainly for gating mechanisms of ion channels and regulation of enzyme catalysis. Ion channels usually feature a gate in the channel pore that prevents ion permeation in the closed state.
unknown ( host institution ) +1 more
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Conformational changes in the catalytically inactive nucleotide-binding site of CFTR. [PDF]
, 2013 A central step in the gating of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel is the association of its two cytosolic nucleotide-binding domains (NBDs) into a head-to-tail dimer, with two nucleotides bound at the ...
Mihályi Csaba +19 more
core +1 more source
Sodium channel inactivation kinetics of rat sensory and motor nerve fibres and their modulation by glutathione [PDF]
, 1993 Na+ channel currents of rat motor and sensory nerve fibres were studied with the patch-clamp technique on enzymatically demyelinated axons. Differences between motor and sensory fibres in multi-channel inactivation kinetics and the gating of late single ...
Quasthoff, Stefan +2 more
core +1 more source