Results 301 to 310 of about 311,648 (345)

Linkers: A synergistic way for the synthesis of chimeric proteins

Protein Expression and Purification, 2022
In the cell, the protein domains are attached with the short oligopeptide, commonly known as linker peptide. Besides bridging, the linker assists in the domain-domain interaction and protein folding into the peculiar conformations. Linkers allow or control the movement of protein domains in the dynamic cellular environment.
Dharti Keyur Patel, Dhanya Vettiatil, Darshan H. Patel, Gayatri Dave   +3 more
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Striking gold with chimeric proteins

Nature Catalysis, 2021
The ability to control the subtle differences in reaction mechanisms and outcomes is an aspiration of many synthetic chemists. Now protein evolution has enabled the control of selectivity for hydroamination reactions catalysed by gold-based artificial metalloenzymes by favouring dual-gold catalysis over monomeric catalysis.
openaire   +1 more source

Engineering Bacterial Microcompartment Shells: Chimeric Shell Proteins and Chimeric Carboxysome Shells

ACS Synthetic Biology, 2014
Bacterial microcompartments (BMCs) are self-assembling organelles composed entirely of protein. Depending on the enzymes they encapsulate, BMCs function in either inorganic carbon fixation (carboxysomes) or organic carbon utilization (metabolosomes).
Fei, Cai, Markus, Sutter, Susan L, Bernstein, James N, Kinney, Cheryl A, Kerfeld   +4 more
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Fungal Protein Production: Design and Production of Chimeric Proteins

Annual Review of Microbiology, 2011
For more than a century, filamentous fungi have been used for the production of a wide variety of endogenous enzymes of industrial interest. More recently, with the use of genetic engineering tools developed for these organisms, this use has expanded for the production of nonnative heterologous proteins.
Punt, Peter J., Levasseur, Anthony, Visser, Hans, Wery, Jan, Record, Eric   +4 more
openaire   +5 more sources

Expression vectors for streptavidin-containing chimeric proteins

Biochemical and Biophysical Research Communications, 1991
We have constructed expression vectors for streptavidin-containing chimeric proteins. These vectors carry the DNA sequence corresponding to the core region of the streptavidin molecule, and have several unique cloning sites which facilitate construction of gene fusions of streptavidin with a target protein.
T, Sano, C R, Cantor
openaire   +2 more sources

Functionality of chimeric Rev proteins of HIV/SIV

Virus Genes, 1995
Studies on functional compatibility of various Rev proteins derived from all known human and simian immunodeficiency virus subgroups have shown that this essential gene product is not always exchangeable among the viruses. In an attempt to map the region of Rev proteins responsible for the observed nonreciprocal complementation, hybrid genomic Rev ...
R A, Furuta, H, Sakai, M, Kawamura, K, Tokunaga, M, Hatanaka, A, Adachi   +5 more
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A fused chimeric protein made in human cells

Cell, 1989
The road between gene and final protein product is long and not without unexpected turns. In extreme cases, the computer translation of the gene sequence bears as little resemblance to the mature protein as tadpoles to frogs. In this issue of Cell, the groups of Yoshida and Kan report a new turn in this road, synthesis of a fused protein.
P, Borst, R, Benne, H F, Tabak
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Engineering of Functional Chimeric Protein G–VargulaLuciferase

Analytical Biochemistry, 1997
Luciferase of Vargula hilgendorfli is infinitely stable at room temperature in dried state, and its light-emitting reaction is very simple. These unique characteristics of Vargula luciferase have prompted us to engineer chimeric protein, the other moiety chosen for conjugation being streptococcal protein G.
Yumi Maeda, Hiroshi Ueda, Jun Kazami, Genji Kawano, Eiji Suzuki, Teruyuki Nagamune   +5 more
openaire   +2 more sources

Chimeric proteins as candidates for cancer treatment

Drugs of the Future, 2002
The rising rate of cancer-related diseases and mortality underscores the need for new approaches to directly target and light cancer. This review summarizes one of these new promising treatments, the use of targeted chimeric proteins. Chimeric proteins are two proteins fused at the DNA level in such a way that, once expressed, they result in a single ...
null Ben-Yehudah, A., null Belostotsky, R., null Lichtenstein, M., null Aqeilan, R., null Abady, R., null Lorberboum-Galski, H.   +5 more
openaire   +1 more source

Identification of Functional Protein Regions Through Chimeric Protein Construction

Journal of Visualized Experiments, 2019
The goal of this protocol encompasses the design of chimeric proteins in which distinct regions of a protein are replaced by their corresponding sequences in a structurally similar protein, in order to determine the functional importance of these regions.
Juan M, Adrian-Segarra, Holger, Lörchner, Thomas, Braun, Jochen, Pöling   +3 more
openaire   +2 more sources