Results 261 to 270 of about 254,758 (298)

Expression vectors for streptavidin-containing chimeric proteins

Biochemical and Biophysical Research Communications, 1991
We have constructed expression vectors for streptavidin-containing chimeric proteins. These vectors carry the DNA sequence corresponding to the core region of the streptavidin molecule, and have several unique cloning sites which facilitate construction of gene fusions of streptavidin with a target protein.
T, Sano, C R, Cantor
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Functionality of chimeric Rev proteins of HIV/SIV

Virus Genes, 1995
Studies on functional compatibility of various Rev proteins derived from all known human and simian immunodeficiency virus subgroups have shown that this essential gene product is not always exchangeable among the viruses. In an attempt to map the region of Rev proteins responsible for the observed nonreciprocal complementation, hybrid genomic Rev ...
R A, Furuta, H, Sakai, M, Kawamura, K, Tokunaga, M, Hatanaka, A, Adachi   +5 more
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Ocular distribution of the chimeric protein IL2-PE40

Current Eye Research, 1995
A construct of IL-2 and pseudomonas exotoxin (PE40) has been genetically engineered. An aliquot of 100 microliter of the chimeric protein, radiolabelled with I125, was administered to healthy rats by various routes. At different intervals, ocular and non ocular tissues were removed and the levels of the radiolabelled chimeric protein IL-2-PE40 measured.
D, BenEzra, G, Maftzir, E, Hochberg, I, Anteby, H, Lorberboum-Galski   +4 more
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A fused chimeric protein made in human cells

Cell, 1989
The road between gene and final protein product is long and not without unexpected turns. In extreme cases, the computer translation of the gene sequence bears as little resemblance to the mature protein as tadpoles to frogs. In this issue of Cell, the groups of Yoshida and Kan report a new turn in this road, synthesis of a fused protein.
P, Borst, R, Benne, H F, Tabak
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Engineering of Functional Chimeric Protein G–VargulaLuciferase

Analytical Biochemistry, 1997
Luciferase of Vargula hilgendorfli is infinitely stable at room temperature in dried state, and its light-emitting reaction is very simple. These unique characteristics of Vargula luciferase have prompted us to engineer chimeric protein, the other moiety chosen for conjugation being streptococcal protein G.
Yumi Maeda, Hiroshi Ueda, Jun Kazami, Genji Kawano, Eiji Suzuki, Teruyuki Nagamune   +5 more
openaire   +2 more sources

Chimeric proteins as candidates for cancer treatment

Drugs of the Future, 2002
The rising rate of cancer-related diseases and mortality underscores the need for new approaches to directly target and light cancer. This review summarizes one of these new promising treatments, the use of targeted chimeric proteins. Chimeric proteins are two proteins fused at the DNA level in such a way that, once expressed, they result in a single ...
null Ben-Yehudah, A., null Belostotsky, R., null Lichtenstein, M., null Aqeilan, R., null Abady, R., null Lorberboum-Galski, H.   +5 more
openaire   +1 more source

Identification of Functional Protein Regions Through Chimeric Protein Construction

Journal of Visualized Experiments, 2019
The goal of this protocol encompasses the design of chimeric proteins in which distinct regions of a protein are replaced by their corresponding sequences in a structurally similar protein, in order to determine the functional importance of these regions.
Juan M, Adrian-Segarra, Holger, Lörchner, Thomas, Braun, Jochen, Pöling   +3 more
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Influence of protein transduction domains on target-specific chimeric proteins

Biochemical and Biophysical Research Communications, 2005
Direct targeting to the cytoplasm and nucleus using protein transduction domains (PTD) has been described to be efficient but non-cell-type-specific, and only has clinical relevance when the molecule is active exclusively in the diseased cell. The use of PTDs is an attractive mechanism to improve drug delivery.
Christopher, Bachran, Iring, Heisler, Hendrik, Fuchs, Mark, Sutherland   +3 more
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Chimeric Molecules to Target Proteins for Ubiquitination and Degradation

2005
Protein degradation is one of the tactics used by the cell for irreversibly inactivating proteins. In eukaryotes, ATP-dependent protein degradation in the cytoplasm and nucleus is carried out by the 26S proteasome. Most proteins are targeted to the 26S proteasome by covalent attachment of a multiubiquitin chain.
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Critical care management of chimeric antigen receptor T‐cell therapy recipients

Ca-A Cancer Journal for Clinicians, 2022
Alexander Shimabukuro-Vornhagen, Boris Böll, Peter Schellongowski   +2 more
exaly