A novel chimeric avidin with increased thermal stability using DNA shuffling. [PDF]
PLoS ONE, 2014 Avidins are a family of proteins widely employed in biotechnology. We have previously shown that functional chimeric mutant proteins can be created from avidin and avidin-related protein 2 using a methodology combining random mutagenesis by recombination
Barbara Taskinen +6 more
doaj +1 more source
Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells [PDF]
, 2019 In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line.
Baiocco, P. +8 more
core +2 more sources
Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease. [PDF]
PLoS ONE, 2016 The performance of serologic tests in chronic Chagas disease diagnosis largely depends on the type and quality of the antigen preparations that are used for detection of anti-Trypanosoma cruzi antibodies. Whole-cell T.
Fred Luciano Neves Santos +8 more
doaj +1 more source
Incorporation of chimeric gag protein into retroviral particles [PDF]
Journal of Virology, 1990 The product of the Rous sarcoma virus (RSV) gag gene, Pr76gag, is a polyprotein precursor which is cleaved by the viral protease to yield the major structural proteins of the virion during particle assembly in avian host cells. We have recently shown that myristylated forms of the RSV Gag protein can induce particle formation with very high efficiency ...
R A, Weldon +3 more
openaire +2 more sources
Bio-Protocol, 2018 In this protocol, we describe a method to design chimeric proteins for specific targeting to the inner envelope membrane (IEM) of Arabidopsis chloroplasts and the confirmation of their localization by biochemical analysis.
Susumu Uehara +2 more
doaj +1 more source
Circumventing antivector immunity: potential use of nonhuman adenoviral vectors [PDF]
, 2014 Adenoviruses are efficient gene delivery vectors based on their ability to transduce a wide variety of cell types and drive high-level transient transgene expression. While there have been advances in modifying human adenoviral (HAdV) vectors to increase
Beard C.W. +30 more
core +3 more sources
Understanding the process of envelope glycoprotein incorporation into virions in simian and feline immunodeficiency viruses [PDF]
, 2014 The lentiviral envelope glycoproteins (Env) mediate virus entry by interacting with specific receptors present at the cell surface, thereby determining viral tropism and pathogenesis.
Affranchino, Jose Luis +1 more
core +4 more sources
Construction and characterization of pyocin-colicin chimeric proteins [PDF]
Journal of Bacteriology, 1996 Chimeric proteins were constructed from pyocin S1 or S2 and colicin E3 or E2, and their characteristics were investigated with special reference to the domain structure. The nuclease domains were interchangeable between two bacteriocins so that a new kind of pyocin, with RNase activity, was created.
M, Kageyama +3 more
openaire +2 more sources
BMC Biotechnology, 2017 Background Development of chimeric Cry toxins by protein engineering of known and validated proteins is imperative for enhancing the efficacy and broadening the insecticidal spectrum of these genes.
Maniraj Rathinam +3 more
doaj +1 more source
Tailoring the Host Range of Ackermannviridae Bacteriophages through Chimeric Tailspike Proteins
Viruses, 2023 Host range is a major determinant in the industrial utility of a bacteriophage. A model host range permits broad recognition across serovars of a target bacterium while avoiding cross-reactivity with commensal microbiota.
Jose Gil +6 more
doaj +1 more source